Publications by authors named "Zane Fink"
Article Synopsis
- - PepSeq is an advanced technique for conducting comprehensive proteomic assays using DNA-barcoded peptides, allowing researchers to study the interactions and specificities of proteins in a highly multiplexed manner.
- - The process involves synthesizing a library of peptide/DNA conjugates from a small serum or plasma sample, enabling epitope-level analysis of antibodies and providing insights into pathogen exposure history.
- - The protocol consists of two key parts: designing and creating the DNA-barcoded peptide libraries and employing these libraries for high-throughput serological testing, with analysis supported by user-friendly software tools for efficient data handling.
The SARS-CoV-2 proteome shares regions of conservation with endemic human coronaviruses (CoVs), but it remains unknown to what extent these may be cross-recognized by the antibody response. Here, we study cross-reactivity using a highly multiplexed peptide assay (PepSeq) to generate an epitope-resolved view of IgG reactivity across all human CoVs in both COVID-19 convalescent and negative donors. PepSeq resolves epitopes across the SARS-CoV-2 Spike and Nucleocapsid proteins that are commonly targeted in convalescent donors, including several sites also recognized in some uninfected controls.
View Article and Find Full Text PDFA high-resolution understanding of the antibody response to SARS-CoV-2 is important for the design of effective diagnostics, vaccines and therapeutics. However, SARS-CoV-2 antibody epitopes remain largely uncharacterized, and it is unknown whether and how the response may cross-react with related viruses. Here, we use a multiplexed peptide assay ('PepSeq') to generate an epitope-resolved view of reactivity across all human coronaviruses.
View Article and Find Full Text PDF