Enhanced glucose-1-phosphate production from corn stover using cellulases with reduced β-glucosidase activity via Trbgl1 gene knockout in Trichoderma reesei Rut C30.

The scarcity of cellulases with low β-glucosidase activity poses a significant technological challenge in precisely controlling the partial hydrolysis of lignocellulose to cellobiose, crucial for producing high-value chemicals such as starch, inositol, and NMN. Trichoderma reesei is a primary strain in cellulase production. Therefore, this study targeted the critical β-glucosidase gene, Trbgl1, resulting in over an 86 % reduction in β-glucosidase activity.