[Special influence of molecular imprinting technology on traditional Chinese medicine theories].

Objective: To investigate the influence of molecular imprinting phenomena of traditional Chinese medicines (TCMs) on basic theories of TCMs according to current situations of molecular imprinting technology and experimental studies.

Method: Fundamental principles of molecular imprinting theory were followed to analyze the material basis for TCM molecules to generate the imprinting phenomena, in order to find other material basis with more direct efficacy in line with the requirements of basic theories and modernization of TCMs.

Result: Compared with single-component structures, TCMs and their compound components could create super-molecular compounds through complexation, composition, chelation, inclusion, neutralization, self-assembly and chemical reaction.

[Synthesis and identification of rutin complete antigen and analysis its immunogenicity].

Objective: Synthesis and identification of complete antigen of rutin, the traditional Chinese medicine active ingredient, and develop rapid detection of rutin using enzyme-linked immunoassay method (ELISA). Immunogenicity of the complete antigen was also studied.

Method: Prepare the complete antigen by sodium periodate solution and identified by UV scanning and SDS-PAGE test.

[Current development of rapid high-throughout determination technology for total components of traditional Chinese medicines and formula and synthetic immunity chip method].

The qualitative and quantitative analysis on traditional Chinese medicine and formula components can be made by chemical and instrumental analysis methods. Of both, the instrumental analysis methods play a dominant role, including HPLC, HPLC-MS, HPLC-NMR, GC, GC-MS, biochemical and biological effect. But because traditional Chinese medicines and formula have complicated components, chemical methods are so unspecific that they shall be used less or with caution.

[Current state of studies on screening method for sensitinogens in injections for traditional Chinese medicine and synthetic immunity method].

Injections for traditional Chinese medicine have over 60 years of history of development and application. In recent years, however, their adverse reactions have been reported one after another. Consequently, studies on screening sensitinogens (sensibiligens) from injections for traditional Chinese medicine have drawn people's attention and become a tough problem all over the world.