Publications by authors named "Zan-Lin Zhang"
Objective: To investigate whether microRNA (miRNA) miR-21 regulates dimethylarginine dimethylaminohydrolase 1 (DDAH1) expression through binding 3'-UTR region directly in human umbilical venous endothelial cells (HUVECs) and to explore whether DDAH1-V2/V3 transcripts can function as microRNA sponge, thereby modulating DDAH1-V1 expression.
Methods: The DDAH1 3'-UTR containing miR-21 recognizing sequence was cloned into PmirGLO dual-luciferase miRNA target expression plasmid to construct PmirGLO-miR-21. The plasmid and miR-21 (at concentrations of 25, 50, 100 nM, respectively) or negative control (100 nM) were co-transfected into HUVECs, luciferase activity was detected at 24 h.
Article Synopsis
- A meta-analysis was conducted to clarify the inconsistent findings regarding the GNB3 c.825C > T polymorphism and its association with overweight/obesity, compiling data from 11 articles with over 10,000 subjects.
- The analysis found a significant link between the GNB3 polymorphism and the risk of overweight/obesity, particularly under a recessive model, and noted specific associations in males and younger age groups.
- Results from the stratification showed that the GNB3 825T allele had a strong connection with overweight when considering various inheritance models, emphasizing a need for further exploration in subgroup variations.
Article Synopsis
- G-protein β-polypeptide 3 (GNB3) is linked to hypertension and drug response, particularly the C825T variant, but its impact on blood pressure response to the drugs telmisartan and amlodipine was unclear.
- A study with 93 essential hypertension patients treated with either telmisartan or amlodipine showed no initial blood pressure differences among GNB3 genotypes, but those with the TT genotype had higher diastolic blood pressure after telmisartan treatment compared to those with at least one C allele.
- The findings suggest that the GNB3 C825T variant influences the effectiveness of telmisartan in lowering blood pressure, but not amlodipine, highlighting the need