Structurally diverse sesquiterpenoids from Sarcandra glabra and their anti-inflammatory activities.

Fifteen sesquiterpenoids, including five previously undescribed monomers with oxidative rearranged skeletons (sarglabenoids A-E, 1-5) and three previously unreported lindenane [2 + 2] dimers (sarglabenoids F-H, 6-8), alongside seven related precursors (9-15), were isolated from the root of Sarcandra glabra. The structures of these compounds were elucidated using a combination of high-resolution electrospray ionization mass spectrometry, one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy, the circular dichroism exciton chirality method, electronic circular dichroism, and nuclear magnetic resonance calculations integrated with DP4+ analysis. Compounds 1 and 2 feature an unique 5/5 spiro ring system, which is likely derived from a pinacol rearrangement of precursor 14.

Influence of cell shape on sonoporation efficiency in microbubble-facilitated delivery using micropatterned cell arrays.

Microbubble-facilitated sonoporation is a rapid, versatile, and non-viral intracellular delivery technique with potential for clinical and ex vivo cell engineering applications. We developed a micropatterning-based approach to investigate the impact of cell shape on sonoporation efficacy. Cationic microbubbles were employed to enhance sonoporation by binding to the cell membrane electrostatically.

Effects of Culture Medium Enrichment with Zinc on Astaxanthin Accumulation in a New Strain of the Microalga .

High Zn concentrations in microalgal cells that produce astaxanthin as a feed additive can reduce the symptoms of malnutrition in aquatic animals. Therefore, in this study, we analysed the effect of Zn in the culture medium on the growth of a newly isolated microalgal strain ZY24. Zn and white light stress altered the pigment content in microalgal cells.

Microbial production of methyl-uranium via the Wood-Ljungdahl pathway.

The misuse of uranium is a major threat to human health and the environment. In microbial ecosystems, microbes deploy various strategies to cope with uranium-induced stress. However, the exact ecological strategies and mechanisms underlying uranium tolerance in microbes remain unclear.

The value of electroencephalography features in the prognostic evaluation of large hemispheric infarction patients at different time intervals.

Background: Large Hemispheric Infarction (LHI) is a devastating disease with high mortality. This study aimed to use electroencephalography (EEG) to evaluate the death risk of LHI patients and identify suitable evaluation time.

Methods: This study retrospectively collected clinical and EEG data from 73 LHI patients, dividing them into death and survival group at discharge.

The Study of SRSF1 Regulates Abnormal Alternative Splicing of BCL2L11 and the Role in Refractory Acute Myeloid Leukemia.

Introduction: Abnormalities in splicing factors, such as mutations or deregulated expression, can lead to aberrant splicing of target genes, potentially contributing to the pathogenesis of acute myeloid leukemia (AML). Despite this, the precise mechanism underlying the abnormal alternative splicing (AS) induced by SRSF1, a splicing factor associated with poor AML prognosis, remains elusive.

Methods: Using strict splicing criteria, we globally screened for AS events in NPMc-positive and NPMc-negative AML samples from TCGA.

Anti-inflammatory lindenane sesquiterpenoid dimers from the roots of Chloranthus holostegius var. trichoneurus.

Two new lindenane-type sesquiterpenoid dimers, chlotrichenes C and D (1 and 2) together with five known lindenane-type sesquiterpenoid dimers (3-7) were isolated from the roots of Chloranthus holostegius var. trichoneurus, a famous natural medicine named as "Sikuaiwa" for subduing swellings and relieving pain. The structures including absolute configuration were elucidated by their 1D and 2D NMR, HRESIMS, and ECD data.

Associations of Serum Isoleucine with Mild Cognitive Impairment and Alzheimer's Disease.

Background: Advances in blood biomarker discovery have enabled the improved diagnosis and prognosis of Alzheimer's disease (AD). Most branched-chain amino acids, except isoleucine (Ile), are correlated with both mild cognitive impairment (MCI) and AD. Therefore, this study investigated the association between serum Ile levels and MCI/AD.

Isolation and biomimetic synthesis of phenylpropionyl phenylethylamines from Chloranthus henryi.

In this study, ten phenylpropionyl phenylethylamines, including five previously undescribed ones (1a/b, 2a/b, and 3), five known analogues (4-8), and two established phenylpropanoids precursors (9, 10) were isolated from the aerial parts of Chloranthus henryi Hemsl. Their structures, including absolute configurations, were determined by high-resolution mass spectrometry, enantio-separation, electronic circular dichroism calculation, and single crystal diffraction. Compounds 1a and 1b were the first examples of natural hetero-[2 + 2] cycloaddition products between phenylpropionyl phenylethylamine and phenylpropene.

Estradiol and Benzo[a]pyrene Co-Exposure Contributes to Lung Cancer Cell A549 Proliferation through AHR/AKT/ERK1/2 Pathway.

Objective: Estrogen may have a certain role in promoting lung cancer caused by tobacco. Our understanding of the carcinogenic effects and mechanisms of carcinogen mixture estrogen is limited and mostly relies on the findings from studying individual factors.

Methods: To test this hypothesis, an study was used to investigate the effects of 17 β-estradiol (E) on benzo[a]pyrene (Bap)-induced lung cancer cell A549 proliferation.

Predictive Value of Skeletal Muscle Function Test Combined with Climbing Test for Postoperative Cardiopulmonary Complications in Patients with COPD and Concurrent Lung Cancer.

Purpose: To explore the predictive value of skeletal muscle function measurement combined with stair climbing test for postoperative cardiopulmonary complications in patients with chronic obstructive pulmonary disease (COPD) and non-small cell lung cancer (NSCLC).

Patients And Methods: A prospective study was conducted from June 2022 to July 2023 at West China Hospital of Sichuan University, including 335 COPD patients with lung cancer who underwent surgery. The patients were divided into two groups based on the occurrence of postoperative cardiopulmonary complications: the complication group and the non-complication group.

[Effects of maize and peanut co-ridge intercropping on crop photosynthetic characteristics and intercropping advantages].

To clarify the photosynthetic mechanism contributing to the enhancement of intercropping advantages through co-ridge intercropping of maize and peanut, we conducted a field randomized block experiment under two phosphorus levels of 0(P) and 180 kg PO·hm(P) with flat intercropping of maize and peanut (FIC) as the control. We analyzed the effects of co-ridge intercropping of maize and peanut (RIC) and groove-ridge intercropping of maize and peanut (GIC) on crop leaf area index (LAI), SPAD values, CO carboxylation ability, photosystems coordination (), and intercropping advantage of yield. The results showed that RIC significantly increased SPAD value at the silking stage of intercropping maize, and significantly improved the apparent quantum yield of photosynthesis (AQY), maximum electron transfer rate (), maximum rate of Rubisco carboxylation (), net photosynthetic rate at the CO saturation () and of intercropping maize compared with those of FIC and GIC at silking stage and milking stage, but reduced the ratio of variable fluorescence to amplitude -() and the ratio of variable fluorescence to amplitude -() of the functional leaf photosystem Ⅱ (PSⅡ) at the milking stage of maize.

FA6 Affects Zebrafish Lipid Metabolism through Promoting Acetyl-CoA Synthesis and Inhibiting β-Oxidation.

The intestinal microbiota contributes to energy metabolism, but the molecular mechanisms involved remain less clear. Bacteria of the genus regulate lipid metabolism in the host and are thus commonly used as beneficial probiotic supplements. In the present study, FA6 was selected to assess its role in modulating lipid metabolism of zebrafish ().

Cell mechanical responses to subcellular perturbations generated by ultrasound and targeted microbubbles.

The inherently dynamic and anisotropic microenvironment of cells imposes not only global and slow physical stimulations on cells but also acute and local perturbations. However, cell mechanical responses to transient subcellular physical signals remain unclear. In this study, acoustically activated targeted microbubbles were used to exert mechanical perturbations to single cells.

Construction of automated high-throughput screening method for finding efficient 3-ketosteroid 1,2-dehydrogenating strains.

Dehydrogenation reaction at C1(2) positions is typical and representative of industrial production of steroid drugs. Anti-inflammatory activity can be doubled when the nucleus of the anti-inflammatory steroid hormone drug introduces double bonds at the C1(2) positions. Arthrobacter simplex is currently the most widely studied and used strain for C1(2) dehydrogenation.

Effect of urban traffic-restriction policy on improving air quality based on system dynamics and a non-homogeneous discrete grey model.

Unlabelled: With the acceleration of urbanization, traffic congestion and vehicle exhaust pollution are becoming increasingly serious problems. Focusing on the problem of urban pollution from vehicle exhaust, this study used system dynamics to establish an urban congestion mitigation and emission-reduction management model. Specifically, a nonlinear function that integrates system dynamics and a non-homogeneous discrete grey model (SD-NDGM) was used to construct an algorithm, which improved the accuracy of the model.

Pseudonocardia humida sp. nov., an Actinomycete Isolated from Mangrove Soil Showing Distinct Distribution Pattern of Biosynthetic Gene Clusters.

A novel actinomycete strain, designated S2-4, was isolated from a mangrove soil sample, and a polyphasic approach was employed to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene indicated that strain S2-4 formed a unique clade next to that harboring Pseudonocardia dioxanivorans CB1190, which shared the highest sequence similarity (98.20%) with the new isolate.

Efficient repeated batch production of androstenedione using untreated cane molasses by Mycobacterium neoaurum driven by ATP futile cycle.

The biotransformation of phytosterol to androstenedione (AD) by mycobacteria is a unique process accompanied by energy-producing. However, high intracellular ATP content can severely inhibit the efficient production of AD. In this study, a novel citrate-based ATP futile cycle (AFC) and pyruvate-based AFC were constructed for the first time.

Photovoltaic splitting of water microdroplets on a y-cut LiNbO:Fe crystal coated with oil-infused hydrophobic insulating layers.

We demonstrate the successful photovoltaic splitting of water microdroplets on a $y$y-cut ${{\rm LiNbO}_3}:{\rm Fe}$LiNbO:Fe substrate coated with an oil-infused hydrophobic layer. The temporal evolution of the microdroplet contact angle upon a central illumination and the distinct behaviors of two sub-droplets during a following boundary illumination reveal that both electrowetting and electroosmotic effects induced by the dipolar photovoltaic potential on the substrate contribute to the water microdroplet splitting. The reciprocal relationship between the splitting time and the illumination intensity verifies the inherent photovoltaic nature of the water microdroplet splitting.

Visible-light-assisted condensation of ultrasonically atomized water vapor on LiNbO:Fe crystals.

Optically massive trapping of the moisture in the air into an adjacent surface is a potential technique in the fields of bacterial adhesion and microfluidic generation, which is quite important to the development of LN-based biological lab-on-chips. Here we demonstrate on a LiNbO:Fe substrate the visible-light-assisted condensation of the water vapor in a flowing stream created by an ultrasonic atomizer. Through analyzing the dynamic processes of the visible-light-assisted water condensation at different illumination intensities, it is found that the extent of the water condensation, the bending angle of water vapor trails and the interaction range of the condensation effect are highly dependent on the illumination intensity.

All-optical splitting of dielectric microdroplets by using a y-cut-LN-based anti-symmetrical sandwich structure.

We demonstrate an all-optical active mode of dielectric microdroplet splitting in a sandwich structure consisting of two anti-symmetrical y-cut LN:Fe substrates. The dynamic process of the microdroplet splitting and the simulation of the electrostatic interaction inside the sandwich gap show that the combination of two anti-symmetrical substrates are capable to provide a sufficient dielectrophoretic force and to reduce the unbalance of the drag forces for a stable and efficient splitting of the microdroplet. The dependences of the splitting time on the illumination intensity and the initial microdroplet size are also studied, and the results show that the microdroplet splitting process is fully governed by the establishment of the superposed photovoltaic field inside the sandwich gap.

Laser phase noise effect and reduction in self-homodyne optical OFDM transmission system.

We present a laser phase noise (PN) induced effect of a phase-modulation-to-intensity-modulation conversion noise and noise pedestals underneath each of the orthogonal frequency division multiplexing (OFDM) subcarriers in a self-coherent optical OFDM transmission using a self-homodyne technique. We provide a statistical analysis on the received symbols using a histogram to demonstrate the effect of a phase rotation term and inter-subcarrier interference individually and collectively. The PN is then compensated using a simple time delay to realign the phase walk-off of the subcarriers relative to the carrier.

Effects of nitrogen addition and mowing on rodent damage in an Inner Mongolian steppe.

Rodent damage is a serious threat to sustainable management of grassland. The effects of nitrogen (N) deposition and grassland management on rodent damage have been scarcely studied. Here, we reported the effects of 2 years of N addition and mowing on burrow density and damage area of in a semiarid steppe in Inner Mongolia.

Direct laser writing combined with a phase-delay probe.

We develop on lithium niobate crystals a photorefractive direct-laser-writing approach, in which we combine in one beam both direct writing and phase-delay probing functionalities to extract the in situ information of the refractive index or the electrostatic field. The phase-delay signal, predicted well by the photorefractive theory, is used as feedback for tuning the exposure time or scanning speed of the focused laser in order to control the refractive index change (Δn) at single points and scanning lines. Different features found in creating Δn at the points and lines are explained by the different photorefractive responses in the two cases.

Impact of the crystal orientation of Fe-doped lithium niobate on photo-assisted proton exchange and chemical etching.

Photo-assisted proton-exchange (PAPE) is carried out on the +c- and y-surfaces of Fe-doped LiNbO crystals and the impact of the crystal orientation on the PAPE and the subsequent photo-assisted chemical etching (PACE) is investigated. The proton distributions and the morphologies of the proton-exchanged surfaces are studied by using Micro-FT-IR, Micro-Raman, optical and scanning electron microscopy. Through the PAPE process the proton-exchange can be confined in a specific region by an incident laser beam with fixed intensity profile.