Massage therapy protocol for post-anterior cruciate ligament reconstruction patellofemoral pain syndrome: a case report.

Background: The intent of the present study was to determine the effectiveness of massage therapy in the rehabilitation of post-anterior cruciate ligament reconstruction patellofemoral pain syndrome. The primary complications following surgical repair of the anterior cruciate ligament-classified as patellofemoral pain syndrome-are hamstring flexion contracture and quadriceps weakness, leading to patellofemoral dysfunction and retropatellar pain.

Methods: Treatment included lymphatic drainage, myofascial release, neuromuscular techniques including trigger point release, muscle energy techniques and cross-fiber friction.

Maturation of a 100 kDa protein associated with preribosomes in Chinese hamster ovary cells.

A 100 kDa nucleolar protein which is transitorily associated with preribosomes in the nucleoli of Chinese hamster ovary cells has been found to be specifically cleaved by a thiol protease. During an 'in vitro' incubation of nucleoli, the 100 kDa protein is processed into eight different proteins which are detected by immunoreaction with a serum raised against the 100 kDa protein. Qualitative and quantitative variations in the maturation products of the 100 kDa protein are obtained by 'in vitro' incubation of the 60S and 80S preribosomes.

Unusual processing of nucleolar RNA synthesized during a heat shock in CHO cells.

Maturation of pre-rRNA has been investigated through heat shock experiments in which pre-rRNA synthesis is successively turned off and turned on. After one hour at 43 degrees C high molecular weight RNA is no longer synthesized and both the methylation and the maturation of pre-rRNA synthesized before heat shock are blocked. After two hours recovery at 37 degrees C, methylation and simultaneous maturation of pre-existing RNA occur while pre-rRNA synthesis is reinitiated only after 7 hours at 37 degrees C.

Synthesis and behaviour of small RNA species of CHO cells submitted to a heat chick.

Incubation of Chinese hamster ovary (CHO) cells for 1 hour at 43 degrees C results in an inhibition of high molecular weight RNA synthesis while most of the ow molecular weight RNAs are still synthesized. In cells returned to 37 degrees C, the transcription of high molecular weight RNA is reinitiated after 7 h recovery. The synthesis of snRNA A, C, D which are transcribed by RNA polymerase B (II) is inhibited in cells incubated at 43 degrees C while the synthesis of 4S, 5S, L and K components is not affected.

Effects of heat shock on nuclear and nucleolar protein phosphorylation in Chinese hamster ovary cells.

Distribution and 32P labeling of nuclear and nucleolar phosphoproteins were studied in Chinese hamster ovary cells incubated at supranormal temperature (1 h at 43 degrees C). The heat shock induced the phosphorylation of a nucleolar protein with a molecular weight of 95,000. Similarly, in the non-nucleolar fraction of the nucleus, phosphorylation of a 54,000-Mr protein was induced while a protein with a molecular weight of 35,000 was rapidly dephosphorylated.

Effects of heat shock on gene expression and subcellular protein distribution in Chinese hamster ovary cells.

Incubation of Chinese Hamster Ovary (CHO) cells for one hour at 43 degrees C results in several obvious changes in protein distribution and protein synthesis. One major protein of the cytoplasm (molecular weight 45,000 daltions), also present as a minor component in the nucleus, rapidly disappeared while several proteins, especially high molecular weight peptides, were induced by heat shock. Localization of the proteins in the cytoplasm, extra-nucleolar chromatin and nucleolar bodies has been carried out.

[Transcription complexes in subnuclear fractions isolated from mammalian cells: ultrastructural study].

Miller Beatty's technique was adapted to the study of definite chromatin fractions (nucleolar and nonnucleolar chromatin) isolated from Mammalian cells. The ultrastructural organization of the transcriptional complexes obtained depended on the nuclear compartment studied. In isolated nucleoli, there were "Christmas-tree"-like figures.

[Inhibitory effects of homospecific non-histone chromatin proteins on morphogenesis of urodele amphibian embryos].

Non-histone chromosomal proteins (NHCP) were injected into the blastocoel of advanced blastulae fromPleurodeles waltlii which were previously punctured. NHCP extracted from the liver of the same amphibian species bring very strongly inhibitory effects on morphogenesis: gastrulation is prevented in 75% of cases, but the embryos can survive for 8 to 10 days without showing any necrotic cells, and cell divisions still occur sporadically. Intercellular adhesivity is strongly impaired in such embryos, in connection with inhibition of morphogenetic movements.

Autoradiographic study of the penetration of non-histone chromatin proteins into differentiating cells.

Attention has previously been drawn to a specific effect of NHCP on embryonic Pleurodeles cell differentiation. With a modified NHCP labelling technique, autoradiography has revealed a cytoplasmic concentration of labelled NHCP and has not revealed any difference between homospecific and heterospecific NHCP penetration.

Ultrastructural organization and biochemical characterization of chromatin - RNA - protein complexes isolated from mammalian cell nuclei.

Extranucleolar elements were isolated from mammalian cell nuclei and characterized by correlative biochemical-ultrastructural studies. These complex structures were shown to retain a supra-particle arrangement closely resembling the organization in situ of definite nuclear areas. In particular the morphological RNA - protein species, namely perichromatin fibrils and granules, were detected in association with characteristic regions of chromatin.

Rat hepatoma cells nucleolar DNA. II. A possible model of nucleolar DNA organisation.

A model of nucleolar DNA organization has been established. Three clearly defined main components are found in ascites hepatoma cell nucleolar DNA by CsCl gradient analysis. A linear arrangement for nucleolar DNA and a model of DNA organization in the neighbourhood of a set of ribosomal genes, which may play a fundamental role in the elaboration of nucleolar chromatin tertiary structure, are presented.

Rat hepatoma cells nucleolar DNA. 2. Analysis of nucleolar DNA.

The analysis by CsCl density gradient of nucleolar DNA has revealed that the 1.700 g/cm3 main component can be subdivided in three subcomponents with buoyant densities of 1.707 g/cm3, 1.

[Biological effects of solutions of non-histone chromatin-associated proteins on cell differentiation in vitro (author's transl)].

Nuclear non-histone (NNH) proteins, both chromatin-associated and otherwise, have been prepared in defined conditions, either from nuclei or from washed chromatin. When these NNH proteins are homospecific, they produce inhibitory effects on morphological differentiation of embryonic Urodelan Amphibian cells cultivated in vitro. When they are heterospecific, on the contrary, they have no action on the differentiation of those cells.

Early nucleolar preribosomal RNA - protein in mammalian cells.

Early steps of ribosomal maturation have been studied by analysis of nucleolar extracts using different extraction procedures. Early 45-S nucleolar RNA is found to be associated with slowly sedimenting elements, distinct from previously described 80-S and 55-S nucleolar preribosomes. This early 45-S RNA has been shown to be of preribosomal type according to the following criteria.

[Tissue specificity of effects of non histone chromosomal proteins on differentiation of embryonic organs in culture].

A solution of non-hostone chromatin proteins, extracted from chicken livers, stimulates hepatic tissue differentiation in vitro, but is toxic or without effect (depending on the stage at which the explants are removed) on cardiac tube development. These results provide evidence for tissue-specific effects of these proteins.