Publications by authors named "Zakharchenko V"

Traumatic brain injury (TBI) is the leading cause of global mortality and morbidity. Because TBI is accident-related, the role of genetics in predisposing to TBI has been largely unexplored. However, the likelihood of injury may not be entirely random and may be associated with certain physical and mental characteristics.

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Post-COVID-19 syndrome is a complex of different symptoms, which results in a multisystemic impairment after the suffering from COVID-19 infection. The aim of the study was to reveal the clinical, laboratory, and gut disorders in patients with post-COVID-19 syndrome ( = 39) before and after taking part in the 14-day complex program of rehabilitation. A complete blood count, coagulation test, blood chemistry, biomarkers, and metabolites in serum samples, and gut dysbiosis were revealed in patients on the day of admission and after 14-day rehabilitation, in comparison with the variables of healthy volunteers ( = 48) or with reference ranges.

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The ability to rapidly adapt to new environmental conditions is a crucial prerequisite for the wide-scale invasion of pests or intentional introduction of beneficial insects. A photoperiodically induced facultative winter diapause is an important adaptation ensuring synchronization of insect development and reproduction with the local seasonal dynamics of environmental factors. We conducted a laboratory study aimed to compare photoperiodic responses of two invasive Caucasian populations of the brown marmorated stink bug (Hemiptera: Heteroptera: Pentatomidae), which recently invaded neighboring regions with subtropical (Sukhum, Abkhazia) and temperate (Abinsk, Russia) climates.

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Increasing evidence suggests that gut dysbiosis is associated with coronavirus disease 2019 (COVID-19) infection and may persist long after disease resolution. The excessive use of antimicrobials in patients with COVID-19 can lead to additional destruction of the microbiota, as well as to the growth and spread of antimicrobial resistance. The problem of bacterial resistance to antibiotics encourages the search for alternative methods of limiting bacterial growth and restoring the normal balance of the microbiota in the human body.

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Article Synopsis
  • Vascular endothelial growth factors (VEGFs) are crucial for processes like forming new blood vessels (angiogenesis) and protecting the nervous system, and their imbalance is linked to brain diseases.
  • This study measured serum levels of VEGF-A, VEGFR-1, and VEGFR-2 at different stages of both ischemic (blocked blood flow) and hemorrhagic (bleeding) strokes to see how they change over time.
  • Results showed distinct patterns: ischemic stroke patients had increased VEGFR-2 early on, while hemorrhagic stroke patients had rising VEGF-A levels in later phases, suggesting different biological responses based on the type and timing of the stroke.
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Studies on the phenology of local populations of invasive insects are necessary for monitoring and predicting their dispersion. We investigated the phenology of the brown marmorated stink bug, Halyomorpha halys, in the Sochi region (Krasnodar Territory, Russia) from 2018 to 2021 by regular field sampling and dissecting. The results of the sampling suggest that H.

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Facultative winter adult diapause in is regulated by a long-day photoperiodic response. Day length also influences nymphal development, which slows down at the critical (near-threshold) day lengths. We compared the photoperiodic responses of one native (Andong, South Korea) and three invasive (Torino, Italy; Basel, Switzerland; and Sochi, Russia) populations in a laboratory common-garden experiment.

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Usher syndrome (USH) is the most common form of monogenic deaf-blindness. Loss of vision is untreatable and there are no suitable animal models for testing therapeutic strategies of the ocular constituent of USH, so far. By introducing a human mutation into the harmonin-encoding USH1C gene in pigs, we generated the first translational animal model for USH type 1 with characteristic hearing defect, vestibular dysfunction, and visual impairment.

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Point cloud based immersive media representation format has provided many opportunities for extended reality applications and has become widely used in volumetric content capturing scenarios. The high data rate of the point cloud is one of the key problems preventing the adoption of this media format. MPEG Immersive media working group (MPEG-I) aims to create a point cloud compression methodology relying on the existing video coding hardware implementations to solve this problem.

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Rationale: Recommendations of relevant international organizations controlling the quality of grape wines and beverages specify that only tartaric acids of grape origin can be introduced to achieve the required parameters. The development of methods for determining the origin of tartaric acid in grape wine is of great technological significance.

Methods: Organic dicarboxylic oxyacids were extracted from wines as barium salts.

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We compared data on the extent of bioremediation in soils polluted with oil. The data were obtained using conventional methods of hydrocarbon determination: extraction gas chromatography-mass spectrometry, extraction IR spectroscopy, and extraction gravimetry. Due to differences in the relative abundances of the stable carbon isotopes (13C/12C) in oil and in soil organic matter, these ratios could be used as natural isotopic labels of either substance.

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The hydrocarbon-oxidizing potential of soil microbiota and hydrocarbon-oxidizing microorganisms introduced into soil was studied based on the quantitative and isotopic characteristics of carbon in products formed in microbial degradation of oil hydrocarbons. Comparison of CO2 production rates in native soil and that polluted with crude oil showed the intensity of microbial mineralization of soil organic matter (SOM) in the presence of oil hydrocarbons to be higher as compared with non-polluted soil, that is, revealed a priming effect ofoil. The amount of carbon of newly synthesized organic products (cell biomass and exometabolites) due to consumed petroleum was shown to significantly exceed that of SOM consumed for production of CO2.

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The total values of white blood cells (WBC), the absolute and relative neutrophil counts, neutrophil VCS parameters: MVNe (volume), MCNe (conductivity), and MSNe (light scattering) were studied in 70 patients with recurrent and metastatic cancer of the cervix uteri, by using a LH-500 hematology analyzer (Beckman Coulter). The blood from 35 apparently healthy women served as a control. The magnitude of changes in MVNe and in the intensity of MSNe was found to increase in patients when the level of WBC and the content of neutrophils were elevated.

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Using a theoretical model and mass isotopic balance, biogas (methane and CO(2)) released from buried products at their microbial degradation was analysed in the landfill of municipal and non-toxic industrial solid organic waste near Kaluga city, Russia. The landfill contains about 1.34 x 10(6) tons of waste buried using a 'sandwich technique' (successive application of sand-clay and waste layers).

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Ecological-taxonomic analysis of mycobiota from heavy metal polluted soils in Kryvyi Rig town and its residential suburbs are presented in this study The mycobiota consists of45 filamentous species (127 strains) belonging to 28 genera from 3 divisions. Among them Myrothecium leucotrichum was described as a new species for Ukrainian mycobiota while Absidia cylindrospora and Gongronella butleri as rare species. Paecilomyces lilacinus and P.

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In the article the questions of eating attitudes of people in advanced age are considered. Inspection of 54 people of St. Petersburg in the age from 65 to 84 included questioning (biological, social and questions of eating contents), estimation of eating attitude (the eating attitude test EAT-26, the Dutch eating behaviour questionnaire) and psychological testing (techniques of an estimation of complaints, attitudes, levels of anxiousness, depression, astenia, aggression and quality of life).

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This article describes the role of the intestinal microbiocenosis in the development of metabolic, immunologic, morphologic disorders of the gastrointestinal tract as well as mechanisms of the association of the intestinal endoecology and bacterial metabolites with the mental status, intellectual characteristics and social functions of people.

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Tamm-Horsfall uroprotein accounts for more than 50% of the urinary proteins in healthy individuals. In abnormalities, it creates a favorable background for detecting smaller-sized uroproteins and for diagnosing pathological processes from the results of native urine tests. In this connection, there is a need for precipitating Tamm-Horsfall glycoprotein while applying laser correlation spectroscopy to analyze the size of urine particles in patients with type 2 diabetes mellitus.

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Commonly used clinical and biochemical parameters, such as the content of glucose, insulin, somatotropic hormone, triglycerides, lactate, pyruvate, and free fatty acids (FFA) in blood of practically healthy subjects and in patients with insulin-independent diabetes mellitus (IIDM), were compared with the parameters obtained by mass-spectrometric analysis of 13CO2 in expired air after 13C-glucose loading. It was shown that, as opposed to healthy subjects, the content of blood glucose and free fatty acids in patients with IIDM increased, the level of glucose dropped in progression upon short-term fasting, and the concentration of lactate changed both upon fasting and after the administration of small test doses of glucose. The use of the 13C-glucose breathing test (13C-GBT), which presupposes the loading of safe small doses of glucose enriched in 13C-isotope permitted one to reveal a number of novel quantitative diagnostic criteria for the evaluation of glucose metabolism in patients with IIDM: a decrease in the rate of 13C withdrawal as a constituent of expired carbon dioxide after the administration of 13C-glucose; a reduction in the amount of exogenous glucose metabolized to carbon dioxide; and increased oxidation of endogenous substrates participating in carbon dioxide formation.

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The study deals with a comparative analysis of the relative abundances of the carbon isotopes 12C and 13C in the metabolites and biomass of the Burkholderia sp. BS3702 and Pseudomonas putida BS202-p strains capable of utilizing aliphatic (n-hexadecane) and aromatic (naphthalene) hydrocarbons as sources of carbon and energy. The isotope composition of the carbon dioxide, biomass, and exometabolites produced during the growth of Burkholderia sp.

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