Changes in Soil Bacterial and Nematode Communities during Long-term Continuous Cotton Cropping in an Arid Region.

larifying the effects of continuous cotton cropping (CC) on soil biological communities is essential for maintaining agricultural productivity. In this study, high-throughput sequencing was used to study the effects of different CC durations (0-yr, 5-yr, 10-yr, 15-yr, 20-yr, and 25-yr CC treatments) on soil microbial and nematode communities. The results showed that the dominant bacterial phyla were Actinobacteria and Proteobacteria, and the dominant nematode genus was in all CC treatments.

Dietary Immunoglobulin Y by Targeting Both GbpB and GtfB Enhances the Anticaries Effect in Rats.

Objective: The purpose of this work was to develop an anti-CAT-SYI immunoglobulin Y (IgY) antibody that targeted both GtfB (glucosyltransferase B) and GbpB (glucan-binding protein B) and test its anticaries properties in rats.

Methods: A new CAT-SYI fusion gene was created utilising functional DNA fragments from the GtfB and GbpB genes. The recombinant antigens, comprising the fused CAT-SYI antigen, GtfB, and GbpB, were expressed and purified using a prokaryotic expression and purification system.

Intelligent technologies powering clean incineration of municipal solid waste: A system review.

Cleanliness has been paramount for municipal solid waste incineration (MSWI) systems. In recent years, the rapid advancement of intelligent technologies has fostered unprecedented opportunities for enhancing the cleanliness of MSWI systems. This paper offers a review and analysis of cutting-edge intelligent technologies in MSWI, which include process monitoring, intelligent algorithms, combustion control, flue gas treatment, and particulate control.

annotation of the chemical composition of Tibetan tea and its mechanism on antioxidant and lipid-lowering in mice.

Background/objectives: Tibetan tea is a kind of dark tea, due to the inherent complexity of natural products, the chemical composition and beneficial effects of Tibetan tea are not fully understood. The objective of this study was to unravel the composition of Tibetan tea using knowledge-guided multilayer network (KGMN) techniques and explore its potential antioxidant and hypolipidemic mechanisms in mice.

Materials/methods: The C57BL/6J mice were continuously gavaged with Tibetan tea extract (T group), green tea extract (G group) and ddHO (H group) for 15 days.

Dietary multivalent anti-Helicobacter pylori immunoglobulin Y significantly increase the H. pylori eradication and improve the clinical symptoms in patients.

Background: The oral chicken immunoglobulin Y (IgY) as a novel model of immunotherapy to control Helicobacter pylori (H. pylori, Hp) infection has gained much interest in recent years. However, none of the current IgY therapies showed a total eradication of H.

Telmisartan Attenuates the Growth of Epithelium-like Cells and Glomerular Injury in Spontaneously Hypertensive Rats.

The abnormal growth of epithelium-like cells has been noticed in spontaneously hypertensive rats (SHRs) with hypertensive nephropathy. However, the characteristics of abnormal epithelium-like cells and their pathogenesis in hypertensive nephropathy are not fully understood. In the present study, we investigated the correlation of epithelium-like cells with glomerular injury, and the effects of early drug intervention with telmisartan, an anti-hypertensive drug, on the growth of epithelium-like cells.

Jianqu fermentation with the isolated fungi significantly improves the immune response in immunosuppressed mice.

Ethnopharmacological Relevance: Jianqu, a classical formula of traditional Chinese medicine, is used clinically to treat symptoms like chill and fever headache, diarrhea and loss of appetite and act on patients with low immunity. However, the quality control of Jianqu fermentation is not well established, and its function in regulating the body's immunity still remains unclear.

Aim Of The Study: The present study firstly assesses the structure and diversity of fungal community during Jianqu fermentation and then investigates the immune regulating function of Jianqu extract in mouse model.

Molecular cloning and characterization of the β-1,3-glucan recognition protein in Anatolica polita.

β-1,3-Glucan recognition protein (βGRP) is an important pattern recognition protein, which could trigger immune response to eliminate pathogens by identifying and combining the pathogenic bacteria. In the present study, a β-1,3-glucan recognition protein gene (ApβGRP) was cloned from a desert beetle Anatolica polita based on the EST sequence of ApβGRP in the suppression subtractive cDNA library. Quantitative real-time PCR (qRT-PCR) results showed that ApβGRP transcript in A.

Molecular and functional characterization of ApPGRP from Anatolica polita in the immune response to Escherichia coli.

Peptidoglycan recognition proteins (PGRPs) widely distributed in invertebrates and vertebrates are pattern-recognition molecules in innate immunity. In the present study, a novel short PGRP gene, designated as ApPGRP was identified from Anatolica polita. The deduced amino acid sequence of ApPGRP is composed of 196 residues and contains a conserved PGRP domain at the C-terminus.

Structural and functional profiles of the gut microbial community in polycystic ovary syndrome with insulin resistance (IR-PCOS): a pilot study.

Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder that affects 9-21% of reproductive-aged women. Affected women frequently display obesity, insulin resistance, and inflammation. Altered gut microbial community has been reported in PCOS and obese PCOS patients.

[Preparation of multivalent egg yolk IgY antibodies against Helicobacter pylori].

Objective To prepare a specific multivalent immunoglobulin of yolk (IgY) against multi-virulence factors of Helicobacter pylori (Hp). Methods The purified recombinant BabA2, UerB and FlaA of Hp were mixed equally with adjuvant, and then used to immunize egg-laying hens to produce specific multivalent IgY. Next, the biological characteristics of purified IgY were analyzed by SDS-PAGE, Western blot and ELISA.

Neisseria flavescens: A Urease-Expressing Potential Pathogen Isolated from Gastritis Patients.

Parasitic pathogens, such as H. pylori (Helicobacter pylori), are considered as primary elements for causing stomach infection and leading to chronic gastritis or ulcers. Here, an unreported urease- and oxidase-producing Neisseria flavescens-like bacteria was isolated from the gastroscopic biopsies of C-UBT-positive gastritis patients.

High-grade mutant OmpF induces decreased bacterial survival rate.

OmpF plays very important roles in the influx of antibiotics and bacterial survival in the presence of antibiotics. However, high-grade mutant OmpF and its function in decreasing bacterial survival rate have not been reported to date. In the present study, we cloned a high-grade mutant OmpF (mOmpF) and sequence analysis suggested that over 45 percent of the DNA sequence was significantly mutated, leading to dramatic changes in over 55 percent of the amino acid sequence.

Cordyceps militaris extract attenuates D-galactose-induced memory impairment in mice.

Memory impairment is one of main clinical symptoms of brain senescence. To address the effects of Cordyceps militaris Link extract (CE) on memory impairment, a D-galactose (D-Gal)-induced aging mouse model was employed. Mice injected with D-Gal showed a significant learning and memory impairment that was rescued by CE treatment.

Curcumin induces apoptosis and inhibits growth of human Burkitt's lymphoma in xenograft mouse model.

Curcumin, a natural compound extracted from rhizomes of curcuma Curcuma species, has been shown to possess potent anti-inflammatory, anti-tumor and anti-oxidative properties. However, the mechanism of action of the compound remains poorly understood. In this report, we have analyzed the effects of curcumin on the cell proliferation of Burkitt's lymphoma Raji cells.