Arsenic trioxide induced the apoptosis of laryngeal cancer via down-regulation of survivin mRNA.

Objective: Arsenic trioxide (As(2)O(3)) is used clinically to treat acute promyelocytic leukemia and has activity in vitro against several solid tumor cell lines, where induction of differentiation and apoptosis are the prime effects. As a novel anticancer agent for treatment of solid cancers, As(2)O(3) is promising and the mechanism has been not still fully understood. Laryngeal squamous cell carcinoma (LSCC) is one common tumor in head and neck cancers.

[The value of 99mTc-DX105 cervical lymphatic imaging in detecting cervical lymph node metastasis of laryngeal and hypopharyngeal carcinoma].

Objective: To study the value of single photon emission-computed tomography (SPECT) lymphatic imaging by percutaneous injection of 99mTc-DX105 in detecting cervical lymph node metastasis of laryngeal and hypopharyngeal carcinoma.

Method: One-three days before the operation, 185MBq 99mTc-DX105 was injected via bilateral mastoids portion percutaneously in each of 30 patients with laryngeal and hypopharyngeal carcinoma, and thus SPECT lymphatic imaging was conducted. Then, the diagnostic result was compared with those of pathological examination of neck dissection specimens.

[Long-term results analysis of mastoidectomy for chronic otitis media].

Objective: To study the operative indications of chronic otitis media and their long-term results.

Method: One hundred and forty-five cases of mastoidectomy were followed for 5 years between 1995-1998. We discussed the results of recurrence and hearing level of radical mastoidectomy, canal wall up mastoidectomy (CWUM) and canal wall down mastoidectomy with mastoid obliteration (CWDM).

[Expression and significance of TERT and c-Myc oncogene protein product on cholesteatoma epithelium].

Objective: To detect the expression and localization of human telomerase catalytic subunit (hTERT) and c-Myc oncogene protein product in human middle ear cholesteatoma, and to evaluate their roles in the proliferation of cholesteatoma epithelium.

Method: The specimens from the middle ear cholesteatoma tissue of 32 cases and external ear skin of 14 cases were examined by immunohistochemical SP method and computer image analysis system for TERT and c-Myc oncogene protein product.

Result: All 32 samples of cholesteatoma showed a stronger expression of TERT and c-Myc oncogene protein product than that of the external ear skin.

[Application of chlorophyll in the cervical lymph nodes dissection of laryngeal and hypopharyngeal carcinomas].

Objective: To investigate the patterns of cervical lymph node metastases from the carcinomas of larynx and hypopharynx.

Method: Chlorophyll solution was injected submucosally into some portions of the larynx and hypopharynx preoperately as a stain of lymphatic system to help conduct modified neck dissections in 50 patiants with laryngeal or hypopharyngeal carcinomas. Searial sections were performed.

[The role of high-resolution CT in the preoperative assessment of chronic otitis media].

Objective: To assess the high-resolution CT scan in depicting the middle ear structures.

Method: The surgical findings of 51 ears operated on were retrospectively compared with the CT findings. The followings were analysed: diagnostic features of chronic otitis media (COM) on CT status of the middle ear structures (ossicles, facial nerve canal, semicircular canals and tegmen tympani), and anatomical variations.