[Physical rehabilitation for ankle joint injuries in the elderly and senile (stage 3).].

Ankle injuries are among the most common musculoskeletal injuries in the elderly. However, among the new scientific publications, both foreign and domestic, there are almost no examples and algorithms for conducting rehabilitation exercises for elderly patients, which prevents the introduction of these practices into rehabilitation measures. The purpose of our study is to evaluate the created technique of physical rehabilitation and, with a reliable result, to introduce it into the daily work of doctors of various profiles who provide assistance to patients with ankle joint injuries.

[Bile duct repair after complete intersection].

Objective: To determine the training period for imposing hepaticojejunostomy for bile duct repair.

Material And Methods: Reconstructive surgery on the bile ducts was performed in 53 patients. We analyzed the learning curves based on the incidence of anastomotic leakage after hepaticojejunostomy.

Excitation Intensity- and Size-Dependent Halide Photosegregation in CsPb(IBr) Perovskite Nanocrystals.

Although broad consensus exists that photoirradiation of mixed-halide lead perovskites leads to anion segregation, no model today fully rationalizes all aspects of this near ubiquitous phenomenon. Here, we quantitatively compare experimental, CsPb(IBr) nanocrystal (NC) terminal anion photosegregation stoichiometries and excitation intensity thresholds to a band gap-based, thermodynamic model of mixed-halide perovskite photosegregation. Mixed-halide NCs offer strict tests of theory given physical sizes, which dictate local photogenerated carrier densities.

THE DYNAMICS OF LIPID METABOLISM IN PATIENTS WITH MORBID OBESITY DEPENDING ON THE TYPE OF PERFORMED SURGERY.

The objective of the study - to determine changes in the lipidogram (total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol) as a component of the metabolic syndrome in patients suffering from morbid obesity after bariatric surgery (biliopancreatic bypass surgery, laparoscopic gastric banding, laparoscopic longitudinal gastric resection). The study was based on 166 clinical cases. To achieve this goal, patients were divided into three groups according to the type of surgery underground.

Fusion Stage of HIV-1 Entry Depends on Virus-Induced Cell Surface Exposure of Phosphatidylserine.

HIV-1 entry into host cells starts with interactions between the viral envelope glycoprotein (Env) and cellular CD4 receptors and coreceptors. Previous work has suggested that efficient HIV entry also depends on intracellular signaling, but this remains controversial. Here we report that formation of the pre-fusion Env-CD4-coreceptor complexes triggers non-apoptotic cell surface exposure of the membrane lipid phosphatidylserine (PS).

[PROFILES OF CYTOKINES IN MICE DURING IMMUNIZATION WITH ADTP- VACCINE WITH ACELLULAR PERTUSSIS COMPONENT].

Aim: Study cytokine status in mice immunized with vaccines containing acellular pertussis component.

Materials And Methods: Vaccines developed in Mechnikov RIVS - acellular pertussis vaccine (aPV) and adsorbed pertussis-diphtheria-tetanus vaccine (aDTaP), containing a complex of protective antigens of pertussis microbe - were used in the study. F₁ (CBAxC₅₇B1₆) line mice weighing 12 - 14 g were immunized intraperitoneally 3 times at an interval of 7 days with aPV and aDTaP at human immunization dose (0.

[Elimination on Power Line Interference from ECG Signal Using Combined Double-sided Narrow-band Notch Filter].

The aim of current research was to find negative effects caused by eliminating power line interference in ECG signal with narrow-band recursive notch filter and to search for a filtering method that will solve a problem of signal distortion. During the study the method of ECG filtering with narrow-band recursive filter was described. The main negative attribute of this filter is a transient noise, also called "ringing" effect, as the reaction to a single impulse visible as R spikes on the ECG signals.

Efficient entry of cell-penetrating peptide nona-arginine into adherent cells involves a transient increase in intracellular calcium.

Understanding the mechanism of entry of cationic peptides such as nona-arginine (R9) into cells remains an important challenge to their use as efficient drug-delivery vehicles. At nanomolar to low micromolar R9 concentrations and at physiological temperature, peptide entry involves endocytosis. In contrast, at a concentration ≥10 μM, R9 induces a very effective non-endocytic entry pathway specific for cationic peptides.

[Immunogenicity and safety of vaccine preparations based on circulating Bordetella pertussis strains].

Aim: Study specific activity and safety ofvaccine preparations based on circulating B. pertussis strains with currently predominating allele variants of pertussis toxin (ptxA1) and pertactin (prn2) genes.

Materials And Methods: B.

[Immunobiological properties of Bordetella pertussis lipopolysaccharide in the acellular pertussis vaccine].

Aim: Study of Bordetella pertussis lipopolysaccharide (LPS) immunobiological properties in the acellular pertussis vaccine.

Materials And Methods: Experimental series of acellular pertussis vaccines (APV), lyophilized LPS were used. Antibody titers against LPS in mice sera were evaluated by using EIA with peroxidase conjugate of anti-species antibodies against mice IgG.

[Preclinical studies of an adsorbed diphtheria-tetanus-pertussis vaccine (ADTP-vaccine) with acellular pertussis component].

Aim: Evaluate standardness of antigenic composition of pertussis component, completeness of sorption of pertussis, diphtheria and tetanus components, specific activity and safety of experimental series ofADTP-vaccine with acellular pertussis component (ADTaP-vaccine).

Materials And Methods: The content of separate antigens (pertussis toxin, filamentous hemagglutinin and agglutinogens 1, 2, 3) in samples of acellular pertussis component of ADTaP-vaccine and completeness of sorption of pertussis component of ADTaP-vaccine were evaluated by using enzyme immunoassay. Completeness of sorption of diphtheria and tetanus components were determined in flocculation reaction and antitoxin-binding reactions, respectively.

[Epidemic process and vaccine prophylaxis of pertussis].

Analysis of data on epidemic process of pertussis infection in conditions of wide coverage of population by prophylactic vaccination is presented. Post-vaccination immunity against pertussis was shown to reduce with age; therefore a large part of vaccinated children becomes susceptible to this infection already in primary school age. Epidemic process of pertussis continues both as manifest form and as subclinical and atypical forms.

[Evaluation of different methods of hemostasis when performing laparoscopic partial nephrectomy].

The experience of performing 44 partial nephrectomies using laparoscopic and lumboscopic approaches is presented. A comparative analysis of the effectiveness of different methods of hemostasis when performing nephron sparing interventions was conducted. It is shown that physical methods of hemostasis do not meet the necessary requirements, increasing warm ischemia time and the risk of intraoperative blood loss, and makes it difficult to visualize the boundaries of resection.

[First experience of monoport laparoscopic surgery in the treatment of kidney diseases].

The results of research on the effectiveness of monoport laparoscopic surgery for kidney disease are presented. Operations using LESS method (Laparo-Endoscopic Single-Site Surgery) were performed in 21 patients, including 14 nephrectomies and 7 cyst resections. In five cases, nephrectomy was performed due to terminal hydronephrosis, in two cases--due to presence of arteriolosclerotic kidney, in seven cases--due to renal cell carcinoma (T1a-bN0M0).

Ferroptosis: an iron-dependent form of nonapoptotic cell death.

Nonapoptotic forms of cell death may facilitate the selective elimination of some tumor cells or be activated in specific pathological states. The oncogenic RAS-selective lethal small molecule erastin triggers a unique iron-dependent form of nonapoptotic cell death that we term ferroptosis. Ferroptosis is dependent upon intracellular iron, but not other metals, and is morphologically, biochemically, and genetically distinct from apoptosis, necrosis, and autophagy.

Lassa hemorrhagic fever in a late term pregnancy from northern Sierra Leone with a positive maternal outcome: case report.

Lassa fever (LF) is a devastating viral disease prevalent in West Africa. Efforts to take on this public health crisis have been hindered by lack of infrastructure and rapid field deployable diagnosis in areas where the disease is prevalent. Recent capacity building at the Kenema Government Hospital Lassa Fever Ward (KGH LFW) in Sierra Leone has lead to a major turning point in the diagnosis, treatment and study of LF.

[Isotypes of anti-pertussis antibodies in pertussis patients at different stages of the disease].

Aim: Evaluation of anti-pertussis antibodies in pertussis patients at different stages after the onset of the disease.

Materials And Methods: Levels of IgG, IgG1, IgG2, IgG3, IgG4, IgA and IgM antibodies against the antigen complex of pertussis were evaluated by enzyme immunoassay (EIA). Sera samples were analyzed from 208 pertussis patients examined from week 1 to 10 after the onset of the disease.

[Serological activity of vaccine and circulating Bordetella pertussis strains].

Aim: To study activity of vaccine and circulating strains of Bordetella pertussis in serological reactions with serum samples from healthy vaccinated children and children with pertussis infection.

Materials And Methods: One hundred forty-six serum samples from children with pertussis infection as well as 158 samples from healthy vaccinated children aged 3 - 5 years old were studied. Serologic activity of 3 vaccine strains and 7 strains of B.

[Production of pertussis toxin by Bordetella pertussis strains isolated from patients with whooping cough].

Aim: To assess level of pertussin toxin (PT) production by vaccine strains of Bordetella pertussis and strains isolated from patients with whooping cough.

Materials And Methods: Concentration of PT in supernatants of microbial cultures of 3 vaccine strains and 25 strains of B. pertussis isolated from patients with pertussis in 2001 - 2005 was measured with enzyme immunoassay using gamma-globulin fractions of rabbit antiserum to PT as immunosorbent or included in peroxidase conjugates.

[Antigenic composition and serologic characteristics of domestic acellular pertussis vaccine].

Aim: To assess antigenic composition consistency and serological characteristics of domestic acellular pertussis vaccine.

Materials And Methods: Amount of pertussis toxin, filamentous hemagglutinin, agglutinogens types 1, 2, and 3 in experimental batches of vaccine was measured by enzyme immunoassay. Levels of antibodies to aforementioned antigens as well as to lipopolysaccbaride in serum samples obtained from patients with pertussis and healthy vaccinated children were measured by the same method.

Multiple functions of precursor BDNF to CNS neurons: negative regulation of neurite growth, spine formation and cell survival.

Background: Proneurotrophins and mature neurotrophins elicit opposite effects via the p75 neurotrophin receptor (p75(NTR)) and Trk tyrosine kinase receptors, respectively; however the molecular roles of proneurotrophins in the CNS are not fully understood.

Results: Based on two rare single nucleotide polymorphisms (SNPs) of the human brain-derived neurotrophic factor (BDNF) gene, we generated R125M-, R127L- and R125M/R127L-BDNF, which have amino acid substitution(s) near the cleavage site between the pro- and mature-domain of BDNF. Western blot analyses demonstrated that these BDNF variants are poorly cleaved and result in the predominant secretion of proBDNF.