Intra and inter: Alterations in functional brain resting-state networks in patients with functional constipation.

Background: Functional constipation (FCon), is a symptom-based functional gastrointestinal disorder without an organic etiology and altering brain structure and function. However, previous studies mainly focused on isolated brain regions involved in brain plasticity. Therefore, little is known about the altered large-scale interaction of brain networks in FCon.

LncRNA LINC01315 silencing modulates cancer stem cell properties and epithelial-to-mesenchymal transition in colorectal cancer via miR-484/DLK1 axis.

Long non-coding RNA long intergenic non-protein coding RNA 01315 (LncRNA LINC01315) has been found to be implicated in various cancers, but its role and functions in colorectal cancer (CRC) remain to be addressed. Data on LINC01315 expression in CRC were gathered using bioinformatics analysis, and cancer stem cells (CSCs) were sorted by aldehyde dehydrogenase (ALDH) assay and flow cytometry. Migration, invasion, and stemness of CSCs isolated from CRC cells after transfection were determined by scratch, Transwell, and sphere-formation assays, respectively.

Expression and Clinical Significance of Long-chain Noncoding RNA LINC00261 in Colon Cancer.

Background: To investigate the expression of long non-coding RNA (lncRNA) LINC00261 in colon cancer and its clinical significance.

Methods: The Significance Analysis of Microarrays (SAM) software was used to analyze the data of gene microarray GSE33113 which was downloaded from NCBI GEO Data Sets, and differential expression of lncRNA in colon cancer was screened. The expression of LINC00261 in 138 cases of colon cancer and its adjacent normal tissues were detected by in situ hybridization (ISH).

Gambogic acid suppresses colon cancer cell activity .

The aim of the present study was to elucidate the underlying mechanism of antitumor activity of gambogic acid (GA) in colon cancer. Human colon cancer SW620 cells were divided into five treatment groups, including no-treatment control (NC), low dose GA (10 µg/ml), medium dose GA (50 µg/ml), high dose GA (100 µg/ml) and 5-fluorouracil (10 µg/ml). Differences in cell proliferation, apoptosis and cell cycle, invasion, and migration were measured between groups using MTT, flow cytometry, transwell and wound-healing assays, respectively.

Effective and long-term outcome following ligation of the intersphincteric fistula tract (LIFT) for transsphincteric fistula.

Objective: The purpose of this study was to evaluate the efficacy and long-term outcome of the ligation of the intersphincteric fistula tract (LIFT) procedure for transsphincteric fistula-in-ano.

Methods: A total of 43 patients that were treated with LIFT procedure and had a follow-up time of more than 1 year were included.

Results: The median age was 37.

[Ligation of intersphincteric fistula tract in the treatment of complicated fistula-in-ano].

Objective: To evaluate the efficacy of ligation of intersphincteric fistula tract (LIFT) in the treatment of complex fistula-in-ano.

Methods: Clinical data of 24 patients with complex fistula-in-ano who treated with LIFT in the Affiliated Hospital of Nanjing University of Chinese Medicine from September 2009 to February 2012 were analyzed retrospectively. The operative efficacy and postoperative continence were evaluated.