[The antimicrobial activity and acute toxicity of the polymer salts of gentamycin].

By neutralization of copolymers of N-(2-hydroxypropyl) methacrylamide with acrylic acid and copolymers of N-vinylpyrrolidone with crotonic and p-crotonoylaminophenoxyacetic acids in the presence of gentamycin, water-soluble polymer salts containing from 10 to 25 mass% of gentamycin were obtained. These salts regardless of gentamycin content completely retain high level of antimicrobial activity of gentamycin against Staphylococcus spp. and Escherichia coli and are characterized by less (by more than one order of magnitude) acute toxicity.

Cellulose materials modified by antiseptics and their antimicrobial properties.

Adsorption properties of dressing cellulose materials with respect to surfactant antiseptics were studied. These antiseptics are a complex of the copolymer of N-vinylpyrrolidone and crotonic acid with dimethylbenzylalkylammonium chloride (a synthetic polymer with a wide spectrum of antimicrobial effect) and its low molecular weight analogue (dimethylbenzylalkylammonium chloride). It was established that cellulose materials reversibly adsorb mentioned surfactant antiseptics depending on their concentration in the initial solutions.

[Antibacterial action of the polymeric antiseptic catapol].

Activity of a polymer derivative of catamine AB, the antiseptic catapol, against gram positive and gram negative bacteria was studied. The effect of catapol on structural components of bacterial cells and permeability of bacterial cell membranes was investigated. The possibility of varying the susceptibility of bacteria to catapol and the effect of catapol on bacteria susceptible to antibiotics were evaluated.

[Arthrobacter proteinase and its effect on fibrin thrombi].

Arthrobacteria are producers of proteolytic enzymes. Optimal cultivation conditions were determined for Arthrobacter luteus and the mineral composition of the culture medium providing active synthesis of proteinase was optimized. The proteinase was isolated from the culture liquid of Arthrobacter luteus using preparative ion-exchange chromatography on a carboxyl cation exchange resin KMT.

[The ultrasonic diagnosis of the changes in the kidneys in renal forms of acute kidney failure].

An ultrasonic investigation of the kidneys in patients with renal forms of acute renal failure (32 and 24 with ethylene glycol poisoning and leptospirosis, respectively) recorded in the oligo-anuric phase a specific ultrasonic picture of renal lesion demonstrable as long as full recovery of impaired functions. The picture was characterized by enlargement of the kidneys with good differentiation of the pyramids in enhanced parenchymal echogenicity. Ethylene glycol poisoning is characterized by limited renal mobility and an echo negative halo around them.

[Effect of normal and specific immune sera on neuraminidase activity].

We have got evidence that there is no antigenic relationship reflecting the structural similarity between neuraminidases synthesized by noncholera vibrios and Arthrobacter nicotianae. The cross-reactions between the enzymes and heterological antisera were not observed. Antibodies against the A.

[Effect of the antiseptic merthiolate and its derivatives on fungi causing deterioration].

The fungistatic activity of merthiolate and its two polymerous derivatives have been investigated using as test cultures fungi producing deterioration. The fungicides sorbtion dynamics by the fungus micelium have been studied, toxicity and inhibition rate of the catalase from different sources have been evaluated. The polymerous derivatives have been found to maintain high fungistatic activity of merthiolate, but possess less toxicity.

[Variability of beta-1,3/1,4-glucanglucanohydrolase-forming Streptomyces narbonensis induced by UV rays and mitomycin C].

The effect of mitomycin C and UV on spores before germination and two hours after it was investigated with Streptomyces narbonensis 2a producing beta-1,3/1,4-glucanglucan hydrolase (beta-glucanase). The lethal effect of mitomycin C on the germinating spores was considerably greater than that on the spores. The mutagenic activity of mitomycin C was closely related to the process of spore germination.

[Sorption properties of carboxyl cation exchangers with a bacteriostatic effect].

Sorption properties of new carboxyl cation exchangers containing components of salicylic acid (CST and CMTS) and benzoic acid (CBT and CMTB) were examined with respect to large organic ions. Such cation exchangers were shown to have greater permeability for high molecular weight proteins that sorbents of the Biocarb type. Bacteriostatic properties of the above cation exchangers were studied.

[Effect of hydroacridine derivatives on the sensitivity of polyresistant strains of Staphylococcus aureus and Escherichia coli to antibiotics].

Sensitivity of 4 clinical strains of Staph. aureus and E. coli to 13 hydroacridine derivatives and their combinations with antibiotics, such as benzylpenicillin, ampicillin, semi-synthetic penicillins, streptomycin, chloramphenicol, tetracycline, chlortetracycline, monomycin, oleandomycin and erythromycin was studied.

[Effect of synthetic poly-electrolytes of the cationic type on staphylococcal sensitivity to benzylpenicillin].

The study of the effect of synthetic polyelectrolites of the cationic type on benzylpenicillin resistant staphylococci showed that the cation polyelectrolites induced changes in the cell membrane permeability and increased penicillin absorption by the cells thus increasing sensitivity of the penicillin-resistant staphylococci to the antibiotic. Low inhibitory effect of the polyelectrolites with respect to penicillinase and hyaluronidase was shown. Changes in the membrane apparatus of staphylococcus cells due to the effect of polyelectrolites were found.

[Catalase inactivation during storage in solution and its stabilization by polysaccharides of microbial origin].

It has been experimentally shown that the rate of inactivation of pure catalase in solution does not obey the kinetics of the first order. The kinetics of denaturation taking into account partial stabilization of catalase due to the formation of intermolecular complexes of native molecules with denatured molecules has been derived. Using the equation, the rate of catalase inativation during storage in pure solutions has been calculated from the experimental data.

[Phospholipase C of fungi and staphylococci].

The activity of phospholipase was studied in the cultural broth and cell extract of 112 strains of fungi and yeasts. The endoenzyme was detected in 19 strains of mycelial fungi, the exoenzyme was found in Mucor hiemalis 50 and Aspergillus niger 117. Phospholipase C of M.

Some properties of the protease from Aspergillus terricola.

The protease from Aspergillus terricola (terrilytin) was isolated and purified by the ion exchange method and by gel chromatography. The data on its molecular weight, isoelectric point, N-terminal amino acids were described. The protease included polysaccharide which stabilized the enzyme activity under the storage and lyophylization.

[On the effect of immune and normal sera on catalase].

Effect of specific immune and normal sera on catalase was studied. The sera activated the enzyme, partially protected catalase against UV-irradiation and heating and also against the effect of inhibitors. Antibodies against catalase were observed in the fraction of 7 S gamma-globulins of immune serum.