1,3-xylan is present in the cell walls of some red and green algae and is an important organic carbon in the ocean. However, information on its bacterial degradation is quite limited. Here, after enrichment with 1,3-xylan, the diversity of bacteria recovered from marine algae collected in Hainan, China, was analyzed with both the 16S rRNA gene amplicon sequencing and the culture-dependent method.
View Article and Find Full Text PDFUniversal stress proteins (Usps) exist ubiquitously in bacteria and other organisms. Usps play an important role in adaptation of bacteria to a variety of environmental stresses. There is increasing evidence that Usps facilitate pathogens to adapt host environment and are involved in pathogenicity.
View Article and Find Full Text PDFWei Sheng Wu Xue Bao
August 2009
Unlabelled: Temperature is important factor affecting the bacterial diversity.
Objective: In order to disclose the effect of temperature on the diversity of microorganisms.
Methods: The diversity of microorganisms associated with the sponge Pachychilina sp.
With culture-independent approach, microbial total DNA was directly extracted from Pachychalina sp. Using the total microbial DNA as template, archaeal 16S rDNAs were amplified by PCR with universal primers. Amplified products were cloned into T-vector and secondarily amplified by PCR.
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