[Study of gene expression difference in lung carcinogenesis by cDNA microarray].

Background & Objective: Lung cancer is a kind of disease with high incidence and mortality, however its molecular mechanism is not clear yet. This study was designed to investigate gene expression differences among lung cancer tissues, lung paracancerous tissues,matched peripheral normal lung tissues and the metastases of lymph nodes, and to seek the relatively high expressed genes in lung carcinoma tissues, providing possible theoretical basis for early diagnosis and treatment of pulmonary carcinoma.

Methods: Fresh lung cancer tissue, lung paracancerous tissue, matched normal lung tissue and metastases of lymph nodes were deep-frozen in liquid nitrogen; their total RNA were extracted for reversed transcription cDNA probes, which were labeled and subsequently used to hybridize with cDNA microarray with 588 genes.

[Expression of MMP1 and TIMP1 proteins in lung cancer and its biological significance].

Objective: To investigate the expression of MMP1 and TIMP1 proteins in lung cancer tissues and its biological significance.

Methods: MMP1 and TIMP1 proteins were detected with the immunohistochemical method of avidin-biotin complex in formalin-fixed and routinely paraffin-embeded specimens of 46 patients.

Results: There was no correlation in MMP1 protein expression between normal lung tissues (20%) and lung cancer (52.

[Clinical value of hypertonic glucose injection in the treatment of recurrent pneumothorax].

Objective: To investigate the clinical value of the 50% hypertonic glucose injection in the treatment of recurrent pneumothorax.

Methods: Forty-five patients with recurrent pneumothorax were injected with 60-80 ml of 50% hypertonic glucose into the pleural cavity after air discharge and then were instructed to turn their body to the right and the left quickly.

Results: The curative rate was 100% and the curative effect was satisfactory.