A Digital Health Platform to Deliver Tailored Early Stimulation Programs for Children with Developmental Delays.

Developmental delay is a deviation from the regular development of normative milestones during childhood. Early stimulation is a standardized and straightforward technique to support children with developmental delays (aged 0-3 years) in reaching basic motor skills, which are essential for the execution of everyday activities, such as playing, feeding and locomotion. In doing so, early stimulation reduces the chances of permanent motor impairment, thus allowing the child to live a more functional life.

Critical assessment of the spectroscopic activity assay for monitoring trypsin activity in organic-aqueous solvent.

Solvent-assisted protein digestion involves enzymatic hydrolysis in mixed aqueous-organic solvents. With trypsin, acetonitrile is the modifying solvent of choice, recommended at concentrations from 10 to 80% to improve protein sequence coverage in mass spectrometry. Spectroscopic activity assays employing substrate mimics such as N-benzoyl arginine ethyl ester (BAEE) appear to show a relative enhancement of trypsin activity in mixed solvent systems.