Publications by authors named "Zahra Shad"
Response Surface Methodology Modelling of an Aqueous Two-Phase System for Purification of Protease from Penicillium candidum (PCA 1/TT031) under Solid State Fermentation and Its Biochemical Characterization.
Amaal M Alhelli Mohd Yazid Abdul Manap Abdulkarim Sabo Mohammed Hamed Mirhosseini Eilaf Suliman Zahra Shad
Int J Mol Sci
November 2016
Article Synopsis
- PCA 1/TT031 produces various extracellular proteases, and the study aimed to enhance the purification of these enzymes using an aqueous two-phase system (ATPS) combined with Response Surface Methodology (RSM).
- Optimal conditions for purification were found to be using 9.0% PEG 8000, 5.2% NaCl, and 15.9% sodium citrate, achieving a significant 93% yield and a purification factor of 6.8.
- Further characterization revealed the enzyme has a molecular weight between 100 and 140 kDa, functions optimally at pH 6.0 and 50°C, and could be beneficial for applications in the dairy industry, particularly in cheese ripening.
Use of response surface methodology for partitioning, one-step purification of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031).
Amaal M Alhelli Mohd Yazid Abdul Manap Abdulkarim Sabo Mohammed Hamed Mirhosseini Eilaf Suliman Zahra Shad
J Chromatogr B Analyt Technol Biomed Life Sci
December 2016
This report shows the partitioning and purification of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031) by solid-state fermentation (SSF). In the present analysis, some of the important parameters such as PEG concentration, PEG molecular mass, salt concentration and buffer concentration were optimised through the response surface methodology (RSM). The optimum aqueous two-phase systems (ATPS) environment consisted of 13.
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