Nanogram/mL detection of nortriptyline: Preparation of a molecularly imprinted polymer for spectrophotometric determination of nortriptyline based on multivariate optimization methods.

In this work, the molecularly imprinted polymer was used as a selective sorbent in solid-phase extraction method for the spectrophotometric determination of nortriptyline at 239 nm. Molecularly imprinted polymer was synthesized by pyrrole as a functional monomer in the presence of nortriptyline as a template. Several factors, consist of the concentration of the monomer to template ratio, amount of initiator, stirring rate, reaction time, the pH of the buffer solution, amount of sorbent, loading time, shaking rate of loading, extraction time, and shaking rate of extraction were evaluated due to their effectiveness in the preparation and extraction capability of molecularly imprinted polymer.