Phenotypic diversity of CTCs and tdEVs in liquid biopsies of tumour-draining veins is linked to poor prognosis in colorectal cancer.

Background: Circulating tumour cells (CTCs) and tumour-derived extracellular vesicles (tdEVs) have great potential for monitoring therapy response and early detection of tumour relapse, facilitating personalized adjuvant therapeutic strategies. However, their low abundance in peripheral blood limits their informative value. In this study, we explored the presence of CTCs and tdEVs collected intraoperatively from a tumour-draining vein (DV) and via a central venous catheter (CVC) prior to tumour resection.

Predictors of mortality in patients with COVID-19 by flow cytometry.

Despite the great impact of severe acute respiratory syndrome caused by coronavirus 2 (SARS-CoV-2), we still lack techniques that allow us to anticipate the natural history of the disease in order to avoid or shorten the clinical period of the disease. The values of nine cytokines were measured in COVID-19+ patients admitted to the Hospital Universitario Reina Sofía (HURS) using flow cytometry. The cytokines measured are IL-1ß, IL-6, MCP-1, IP-10, IL-10, IL-8, IL-12, IFN-γ and TNF-α.

It Is Not Just Stress: A Bayesian Approach to the Shape of the Negative Psychological Features Associated with Sport Injuries.

The main objective of this study is to extend the stress and injury model of Andersen and Williams to other "negative" psychological variables, such as anxiety and depression, encompassed in the conceptual model of Olmedilla and García-Mas. The relationship is studied of this psychological macro-variable with two other variables related to sports injuries: the search for social support and the search for connections between risk and the environment of athletes. A combination of classic methods and probabilistic approaches through Bayesian networks is used.

Organ Donation: With Great Power Comes Great Responsibility.

Spain is a world leader in donation and transplantation. This model has been exported to other countries with favorable results. The objective of this study was to compare the actual donation intention rate with the effective donation figures, and analyze the main reasons why families decline organ donation.

Nutritional profile and nutraceutical components of olive ( L.) seeds.

Olive seeds, a potential food by-product from both table olive and olive oil industries, were examined for their overall proximate composition, oil, protein, mineral and phenolic components. Proximate analysis indicates that olive seeds are an unusually rich source of total dietary fibre (≅ 47% dry weight basis, DWB), as well as lipids (≅ 30%) and proteins (≅ 17%). Oil composition shows high levels of oleic (≅ 62% of total fatty acids) and linoleic (≅ 24%) acids, moderate concentrations of tocopherols (≅ 460 mg/kg) and squalene (≅ 194 mg/kg), and relatively high amounts of several sterols and non-steroidal triterpenoids.

Histological Features of the Olive Seed and Presence of 7S-Type Seed Storage Proteins as Hallmarks of the Olive Fruit Development.

The production of olive oil is an important economic engine in the Mediterranean area. Nowadays, olive oil is obtained mainly by mechanical processes, by using the whole fruit as the primary raw material. Although the mesocarp is the main source of lipids contributing to olive oil formation, the seed also contributes to the olive oil composition and attributes.

Identification of novel superoxide dismutase isoenzymes in the olive (Olea europaea L.) pollen.

Background: Among antioxidant enzymes, the superoxide dismutase (SOD) family is a major actor in catalysing the disproportionation of superoxide. Apart from its role as antioxidant, these enzymes have a role in cell signalling, and Cu,Zn-SOD proteins are also major pollen allergens. In order to deepen our understanding of the SOD isoenzymes present in olive pollen and to analyse the molecular variability of the pollen Cu,Zn-SOD family, we carried out biochemical, transcriptomic and localization studies of pollen grains from different olive cultivars and other allergenic species.

Identification and Functional Annotation of Genes Differentially Expressed in the Reproductive Tissues of the Olive Tree ( L.) through the Generation of Subtractive Libraries.

The olive tree is a crop of high socio-economical importance in the Mediterranean area. Sexual reproduction in this plant is an essential process, which determines the yield. Successful fertilization is mainly favored and sometimes needed of the presence of pollen grains from a different cultivar as the olive seizes a self-incompatibility system allegedly determined of the sporophytic type.

Automated identification of reference genes based on RNA-seq data.

Background: Gene expression analyses demand appropriate reference genes (RGs) for normalization, in order to obtain reliable assessments. Ideally, RG expression levels should remain constant in all cells, tissues or experimental conditions under study. Housekeeping genes traditionally fulfilled this requirement, but they have been reported to be less invariant than expected; therefore, RGs should be tested and validated for every particular situation.

Patterns of ROS Accumulation in the Stigmas of Angiosperms and Visions into Their Multi-Functionality in Plant Reproduction.

Accumulation of reactive oxygen species (ROS) in the stigma of several plant species has been investigated. Four developmental stages (unopened flower buds, recently opened flowers, dehiscent anthers, and flowers after fertilization) were analyzed by confocal laser scanning microscopy using the ROS-specific probe DCFH2-DA. In all plants scrutinized, the presence of ROS in the stigmas was detected at higher levels during those developmental phases considered "receptive" to pollen interaction.

Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds.

Olive seeds, which are a raw material of interest, have been reported to contain 11S seed storage proteins (SSPs). However, the presence of SSPs such as 7S vicilins has not been studied. In this study, following a search in the olive seed transcriptome, 58 sequences corresponding to 7S vicilins were retrieved.

ReprOlive: a database with linked data for the olive tree (Olea europaea L.) reproductive transcriptome.

Plant reproductive transcriptomes have been analyzed in different species due to the agronomical and biotechnological importance of plant reproduction. Here we presented an olive tree reproductive transcriptome database with samples from pollen and pistil at different developmental stages, and leaf and root as control vegetative tissues http://reprolive.eez.

Weighted data gravitation classification for standard and imbalanced data.

Gravitation is a fundamental interaction whose concept and effects applied to data classification become a novel data classification technique. The simple principle of data gravitation classification (DGC) is to classify data samples by comparing the gravitation between different classes. However, the calculation of gravitation is not a trivial problem due to the different relevance of data attributes for distance computation, the presence of noisy or irrelevant attributes, and the class imbalance problem.

Cellular localization of ROS and NO in olive reproductive tissues during flower development.

Background: Recent studies have shown that reactive oxygen species (ROS) and nitric oxide (NO) are involved in the signalling processes taking place during the interactions pollen-pistil in several plants. The olive tree (Olea europaea L.) is an important crop in Mediterranean countries.

Treatment of pathological humeral shaft fractures with intramedullary nailing. A retrospective study.

A consecutive series of 22 pathological fractures of the humeral shaft in 21 patients treated at one institution were included in this retrospective study. Patients were treated with anterograde locked intramedullary nailing. Mean follow-up was 22.

Determination of polyphenolic compounds in wastewater olive oil by gas chromatography-mass spectrometry.

A simple and sensitive method for the determination of 21 polyphenolic compounds in wastewater from olive oil production plants is proposed. The method involves a liquid-liquid microextraction (LLME) procedure with ethyl acetate followed by a silylation step. Identification and quantification have been performed by gas chromatography-mass spectrometry (GC-MS).

Determination of bisphenol A (BPA) in the presence of phenol by first-derivative fluorescence following micro liquid-liquid extraction (MLLE).

Bisphenol A (BPA) in the presence of phenol is determined using a method based on first-derivative spectrofluorimetry. The proposed method involves a micro liquid-liquid extraction of sodium chloride saturated water samples with diethyl ether followed by direct fluorimetric analysis of extracts. The excitation spectra of both compounds in diethyl ether are recorded between 200 and 290 nm, with the emission wavelength at 306 nm.

Total hip arthroplasty in heart transplant patients.

Avascular necrosis of the femoral head (AVNFH) is a known complication after heart transplantation. In order to assess the efficacy and complications of cementless total hip arthroplasty (THA) in this population, the authors analysed 24 cementless THAs in 18 patients with advanced AVNFH (stage II affecting more than 15% of the articular surface, stage III and IV according to the Ficat-Arlet classification) after a heart transplant procedure. Average duration of follow-up was 35.

Positive-ion ESI mass spectrometry of regioisomeric nonreducing oligosaccharide fatty acid monoesters: in-source fragmentation of sodium adducts.

Structural characterization and differentiation of a novel group of regioisomeric monolaurate esters of the nonreducing trisaccharides raffinose and melezitose, and the nonreducing tetrasaccharide stachyose has been obtained using positive electrospray ionization (ESI) mass spectrometry with in-source fragmentation. The surfactant nature and high polarity of these compounds make them appropriate analytes for being studied by conventional ESI-MS. The position of the acyl chain in each particular regioisomer has been used as a reporter group that allows unambiguous rationalization of the fragmentation routes of the corresponding natriated molecular ions [M + Na](+).

Differential characteristics of olive pollen from different cultivars: biological and clinical implications.

The olive tree is grown in many parts of the world. Its germplasm is very broad, with 250 varieties in Spain alone. Variations in the ability of pollen to germinate have been studied in detail and show conspicuous differences between varieties.

Multiresidue method for simultaneous determination of quinolone antibacterials in pig kidney samples by liquid chromatography with fluorescence detection.

A new analytical method for simultaneous determination of eight quinolones namely, ciprofloxacin, danofloxacin, difloxacin, enrofloxacin, flumequine, marbofloxacin, oxolinic acid and sarafloxacin, in pig kidney samples was developed. The procedure involves the extraction of the quinolones from the samples by traditional extraction, a step for clean-up and preconcentration of the analytes by solid-phase extraction (SPE) and subsequent liquid chromatography separation with fluorescence detection (LC-FD). The mobile phase was composed of acetonitrile and 10 mM citrate buffer solution of pH 4.

Phase I and pharmacokinetic study of gemcitabine administered at fixed-dose rate, combined with docetaxel/melphalan/carboplatin, with autologous hematopoietic progenitor-cell support, in patients with advanced refractory tumors.

The purpose of this trial was to define the maximum tolerated duration (MTD), dose-limiting toxicity (DLT), regimen-related toxicities (RRT), and pharmacokinetics of gemcitabine infused at a fixed dose rate (FDR) of 10 mg/m2/min, combined with docetaxel/melphalan/carboplatin, using autologous stem cell transplantation (ASCT). The duration of gemcitabine infusion was incrementally escalated as a single treatment on day -6 or as 4 daily infusions on days -5 to -2. Gemcitabine was followed by docetaxel (300 or 350 mg/m2) on day -5, and then melphalan (50 mg/m2/day) and carboplatin (333 mg/m2/day) on days -4 to -2.

Determination of regioisomeric distribution in carbohydrate fatty acid monoesters by LC-ESI-MS.

A new LC-ESI-MS method for characterizing the regioisomeric distribution in carbohydrate monoesters with long-chain fatty acids is described. Sucrose monolaurate mixtures were used for development of the method. The surfactant nature and high polarity of these compounds make them appropriate analytes for being detected by electrospray-ionization mass spectrometry (ESI-MS).