Use of Bulk Segregant Analysis for Determining the Genetic Basis of Azole Resistance in the Opportunistic Pathogen .

A sexual cycle was described in 2009 for the opportunistic fungal pathogen , opening up for the first time the possibility of using techniques reliant on sexual crossing for genetic analysis. The present study was undertaken to evaluate whether the technique 'bulk segregant analysis' (BSA), which involves detection of differences between pools of progeny varying in a particular trait, could be applied in conjunction with next-generation sequencing to investigate the underlying basis of monogenic traits in . Resistance to the azole antifungal itraconazole was chosen as a model, with a dedicated bioinformatic pipeline developed to allow identification of SNPs that differed between the resistant progeny pool and resistant parent compared to the sensitive progeny pool and parent.

Signatures of human European Palaeolithic expansion shown by resequencing of non-recombining X-chromosome segments.

Human genetic diversity in Europe has been extensively studied using uniparentally inherited sequences (mitochondrial DNA (mtDNA) and the Y chromosome), which reveal very different patterns indicating sex-specific demographic histories. The X chromosome, haploid in males and inherited twice as often from mothers as from fathers, could provide insights into past female behaviours, but has not been extensively investigated. Here, we use HapMap single-nucleotide polymorphism data to identify genome-wide segments of the X chromosome in which recombination is historically absent and mutations are likely to be the only source of genetic variation, referring to these as phylogeographically informative haplotypes on autosomes and X chromosome (PHAXs).

Great ape Y Chromosome and mitochondrial DNA phylogenies reflect subspecies structure and patterns of mating and dispersal.

The distribution of genetic diversity in great ape species is likely to have been affected by patterns of dispersal and mating. This has previously been investigated by sequencing autosomal and mitochondrial DNA (mtDNA), but large-scale sequence analysis of the male-specific region of the Y Chromosome (MSY) has not yet been undertaken. Here, we use the human MSY reference sequence as a basis for sequence capture and read mapping in 19 great ape males, combining the data with sequences extracted from the published whole genomes of 24 additional males to yield a total sample of 19 chimpanzees, four bonobos, 14 gorillas, and six orangutans, in which interpretable MSY sequence ranges from 2.

Large-scale recent expansion of European patrilineages shown by population resequencing.

The proportion of Europeans descending from Neolithic farmers ∼ 10 thousand years ago (KYA) or Palaeolithic hunter-gatherers has been much debated. The male-specific region of the Y chromosome (MSY) has been widely applied to this question, but unbiased estimates of diversity and time depth have been lacking. Here we show that European patrilineages underwent a recent continent-wide expansion.

The Y-chromosome tree bursts into leaf: 13,000 high-confidence SNPs covering the majority of known clades.

Many studies of human populations have used the male-specific region of the Y chromosome (MSY) as a marker, but MSY sequence variants have traditionally been subject to ascertainment bias. Also, dating of haplogroups has relied on Y-specific short tandem repeats (STRs), involving problems of mutation rate choice, and possible long-term mutation saturation. Next-generation sequencing can ascertain single nucleotide polymorphisms (SNPs) in an unbiased way, leading to phylogenies in which branch-lengths are proportional to time, and allowing the times-to-most-recent-common-ancestor (TMRCAs) of nodes to be estimated directly.

Structure-function analysis of the presumptive Arabidopsis auxin permease AUX1.

We have investigated the subcellular localization, the domain topology, and the amino acid residues that are critical for the function of the presumptive Arabidopsis thaliana auxin influx carrier AUX1. Biochemical fractionation experiments and confocal studies using an N-terminal yellow fluorescent protein (YFP) fusion observed that AUX1 colocalized with plasma membrane (PM) markers. Because of its PM localization, we were able to take advantage of the steep pH gradient that exists across the plant cell PM to investigate AUX1 topology using YFP as a pH-sensitive probe.

[The influence of socio-demographic and psychological variables on the level of satisfaction of geriatric patients from dental care].

Unlabelled: The increasing geriatric population poses unique treatment challenges for the dental practice. Satisfaction from dental treatment is considered to be an important issue that influences the attitude and cooperation of the geriatric patient. It is associated with the quality of treatment and with different variables, such as physical, emotional, social and financial.

Management of the crying child during dental treatment.

The purposes of this study were: (1) To present parents' attitudes toward their child's crying in the dental environment. (2) To suggest a classification of crying children, and discuss its management implications. One hundred and four parents accompanying their children to dental treatment completed a questionnaire assessing the following: the tendency of the child to cry, the preferred approach of the operator to the crying child, and how the parents perceive their own role in such a case.

Parents' attitudes toward behavior management techniques during dental treatment.

Purpose: This study investigated the attitudes of parents toward behavior management techniques used during dental treatment of children.

Methods: One hundred and four parents who accompanied their children to the Department of Pediatric Dentistry at the Hebrew University, Hadassah Faculty of Dental Medicine in Jerusalem, Israel, participated in the study. The techniques for managing the children's behavior were explained to the parents prior to treatment and parents were present in the operatory during dental treatment.

Attitudes of parents towards their presence in the operatory during dental treatments to their children.

The purpose of the present study was to assess attitudes of parents toward their presence in the operatory, while their children undergo dental treatment. One hundred and four adults, who accompanied their children to dental treatments were asked to complete a questionnaire before the dental visits. The survey consisted of three sections sociodemographic information of the children and family, sociodemographic information of the parents, and preference of parents regarding staying with their children in the operatory, and willingness to assist the dentist when the behavior of their child could not be completely handled by the dentist.

Stress levels and related variables among dental students in Jerusalem, Israel.

The aim of the study was to assess levels of stress and associated socio-demographic variables among dental students in their clinical years in the School of Dental Medicine at the Hebrew University and Hadassah in Jerusalem, and compare the findings with those of a similar study undertaken 10 years previously. The study was carried out among the 4th, 5th and 6th year students because these years involve maximum clinical activity. 112 students out of a population of 120 participated.

One month evaluation of the manually rotating "Bio-Bright" toothbrush for clinical safety and efficiency.

A clinical trial, based on the guidelines of the American Dental Association, was conducted to evaluate the efficacy and safety of Bio-Bright, a new manually rotating toothbrush. The brush was used by 28 adults and compared to a group of 26 adults who used an ADA-accepted toothbrush (Oral-B 35). Examinations were recorded for safety, plaque level, gingival inflammation and gingival bleeding at baseline, 15 days and one month.

Dental anxiety of parents in an Israeli kibbutz population.

The purpose of the present study was to compare the levels of dental anxiety in both parents of children in a kibbutz in Israel. Sixty pairs of parents between 22 and 56 years of age with children aged 1-14 years comprised the study population. The parents were divided into three age groups: 22-34 years, 35-44 years, and 45-56 years.

The influence of social and ethnic factors on dental care habits and dental anxiety: a study in Israel.

The purpose of the present study was to assess the influence of social and ethnic factors on dental care habits and dental anxiety in a group of children resident in Jerusalem, Israel. Four hundred and fifty-six children completed a questionnaire that included 21 questions: nine referred to socio-demographic variables, three concerned the children's dental care habits, and the remaining nine concerned dental anxiety. Social level was determined by the father's occupation and their area of residence.

Trends in the prevalence of dental caries in Israel.

This study's objective was to assess the prevalence of dental caries in five-year-olds and twelve-year-olds in Jerusalem, Israel. A total of 166 children comprised the younger group; and 147 children represented the twelve- to thirteen-year-olds. Examinations took place in the classrooms under natural lighting, using a mouth mirror and a probe.

The prognosis of traumatized permanent anterior teeth with fracture of the enamel and dentin.

The purpose of this study was to examine, in the light of a 2-year follow-up, the diagnostic and prognostic value of vitality tests in teeth which had suffered fracture of the enamel and dentin without pulp exposure, to assess the period in which late pathologic changes may occur, and to determine the recommended time intervals for follow-up examinations. Eighty-four childred, 6 to 14 years of age, with 123 traumatized teeth were included in the study. Of these teeth, 87 percent were "vital" at the initial examination, and most of these remained vital throughout the 2 years.

Epidemiology of dental caries in 5-year-old children in Israel.

The objective of this study was to assess the prevalence of dental caries in the earliest age at which children were organized as a group in the national education system and to find possible associations with variables that may help to identify "groups at risk" in this population. A total of 965 children, 5 years old, were examined. They were selected by a method to form a representative sample of all West Jerusalem compulsory kindergarten classes in 1971.