Maxillofacial haemorrhagic symptoms in emergency department patients: impact of antithrombotics.

Purpose: To investigate the effect of antithrombotics on the occurrence of maxillofacial haemorrhagic symptoms, and to determine if these haemorrhagic symptoms are predictors of maxillofacial fractures.

Method: A prospective cohort study was conducted of consecutive patients with maxillofacial trauma who had been admitted to the emergency department of four hospitals in the Netherlands. This study compared five haemorrhagic symptoms (peri-orbital haematoma, raccoon eyes, epistaxis, subconjunctival ecchymosis, and intra-oral haematoma) between patients not-using (NUA) and using (UA) of antithrombotics, and whether these maxillofacial haemorrhagic symptoms served as predictors for maxillofacial fractures.

Implementing microwell slides for detection and isolation of single circulating tumor cells from complex cell suspensions.

Cell loss during detection and isolation of circulating tumor cells (CTCs) is a challenge especially when label-free pre-enrichment technologies are used without the aid of magnetic particles. Although microfluidic systems can remove the majority of "contaminating" white blood cells (WBCs), their remaining numbers are still impeding single CTC isolation, thus making additional separation steps needed. This study aimed to develop a workflow from blood-to-single CTC for complex cell suspensions by testing two microwell formats.

Maxillofacial Fractures in Electric and Conventional Bicycle-Related Accidents.

Purpose: With the increased use of both e-bike and conventional bicycle, the number of bicycle-related accidents has increased accordingly. To determine whether there are differences in maxillofacial injuries between these 2 types of bicycle accidents, e-bike and conventional bicycle accidents were compared.

Material And Methods: A retrospective cohort study was conducted for all the consecutive patients with maxillofacial injury due to e-bike and conventional bicycle accidents attending the emergency department of 4 hospitals in the Netherlands between May 2018 and October 2019.

Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect.

The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D. One of the challenges is that clearing agents induce tissue shrinkage and the shrinkage rates reported in the literature are incoherent. Here, we report that for a classical clearing agent, benzyl-alcohol benzyl-benzoate (BABB), the shrinkage decreases significantly with increasing sample size, and present an analytical formula describing this.

The clam and the dam: A Bayesian belief network approach to environmental flow assessment in a data scarce region.

The Volta clam, Galatea paradoxa, is a freshwater macrobenthic bivalve which is endemic to the Lower Volta River in Ghana. The range of occurrence of the clam has been influenced by the flow regime in the Lower Volta which is in turn controlled by operation of two dams located upstream. Previous research has documented the changes to the Lower Volta due to the dams and attempts have been made to design environmental flows (e-flows), freshwater flows to sustain ecosystems, to inform the re-operation of the dams.

Intraoperative imaging in pathology-assisted surgery.

The pathological assessment of surgical specimens during surgery can reduce the incidence of positive resection margins, which otherwise can result in additional surgeries or aggressive therapeutic regimens. To improve patient outcomes, intraoperative spectroscopic, fluorescence-based, structural, optoacoustic and radiological imaging techniques are being tested on freshly excised tissue. The specific clinical setting and tumour type largely determine whether endogenous or exogenous contrast is to be detected and whether the tumour specificity of the detected biomarker, image resolution, image-acquisition times or penetration depth are to be prioritized.

Combined transmission, dark field and fluorescence microscopy for intact, 3D tissue analysis of biopsies.

Significance: Currently, tissue biopsies are sectioned into 3- to 5-μm-thick slices that are used for conventional pathology analysis. Previous work by confocal microscopy and light-sheet microscopy has shown that analyzing biopsies intact in three-dimensions (3D) is possible and may lead to a better understanding of cancer growth patterns. Although accurate, these methods require fluorescent staining of the tissue, in addition to tissue clearing.

A system dynamics model to quantify the impacts of restoration measures on the water-energy-food nexus in the Urmia lake Basin, Iran.

Water scarcity exacerbated by growing demand in different sectors has created environmental, social, and economic challenges in the Urmia Lake Basin, Iran. Tackling this problem requires an integrated approach considering the basin as an interconnected system where a change in one sector affects others. Here, a System Dynamics Model is developed to simulate the water-energy-food nexus in the Urmia Lake Basin as a holistic multi-sectoral system and to assess the impacts of proposed lake restoration measures, especially looking for trade-offs.

Attributing the hydrological impact of different land use types and their long-term dynamics through combining parsimonious hydrological modelling, alteration analysis and PLSR analysis.

Understanding the relationship between hydrological processes and environmental changes is important for improved water management. The Geba catchment in Ethiopia, forming the headwaters of Tekeze-Atbara basin, was known for its severe land degradation before the recent success in integrated watershed management. This study analyses the hydrological response attributed to land management change using an integrated approach composed of (i) simulating the hydrological response of Land Use/Cover (LULC) changes; (ii) assessing the alteration of streamflow using Alteration of Hydrological Indicators (IHA); and (iii) quantifying the contribution of individual LULC types to the hydrology using Partial Least Square Regression model (PLSR).

Sequencing of human genomes extracted from single cancer cells isolated in a valveless microfluidic device.

Sequencing the genomes of individual cells enables the direct determination of genetic heterogeneity amongst cells within a population. We have developed an injection-moulded valveless microfluidic device in which single cells from colorectal cancer derived cell lines (LS174T, LS180 and RKO) and fresh colorectal tumors have been individually trapped, their genomes extracted and prepared for sequencing using multiple displacement amplification (MDA). Ninety nine percent of the DNA sequences obtained mapped to a reference human genome, indicating that there was effectively no contamination of these samples from non-human sources.

Sensitive detection of mitochondrial DNA variants for analysis of mitochondrial DNA-enriched extracts from frozen tumor tissue.

Large variation exists in mitochondrial DNA (mtDNA) not only between but also within individuals. Also in human cancer, tumor-specific mtDNA variation exists. In this work, we describe the comparison of four methods to extract mtDNA as pure as possible from frozen tumor tissue.

Quantifying longitudinal land use change from land degradation to rehabilitation in the headwaters of Tekeze-Atbara Basin, Ethiopia.

The spatiotemporal variability of the Land Use/Cover (LULC) is a strong influence on the land management and hydrological processes of a river basin. In particular in semi-arid regions like the Tekeze-Atbara (T-A) basin, accurate information about LULC change is a prerequisite for improved land and water management. The human-induced landscape transformations in the T-A basin, one of the main tributaries of the Nile River, were investigated for the last four decades (1972-2014).

Complete sequence-based pathway analysis by differential on-chip DNA and RNA extraction from a single cell.

We demonstrate on-chip, differential DNA and RNA extraction from a single cell using a microfluidic chip and a two-stage lysis protocol. This method enables direct use of the whole extract, without additional washing steps, reducing sample loss. Using this method, the tumor driving pathway in individual cells from a colorectal cancer cell line was determined by applying a Bayesian computational pathway model to sequences obtained from the RNA fraction of a single cell and, the mutations driving the pathway were determined by analyzing sequences obtained from the DNA fraction of the same single cell.

Towards benchmarking citizen observatories: Features and functioning of online amateur weather networks.

Crowd-sourced environmental observations are increasingly being considered as having the potential to enhance the spatial and temporal resolution of current data streams from terrestrial and areal sensors. The rapid diffusion of ICTs during the past decades has facilitated the process of data collection and sharing by the general public and has resulted in the formation of various online environmental citizen observatory networks. Online amateur weather networks are a particular example of such ICT-mediated observatories that are rooted in one of the oldest and most widely practiced citizen science activities, namely amateur weather observation.

Inequalities in microbial contamination of drinking water supplies in urban areas: the case of Lilongwe, Malawi.

Over past decades strategies for improving access to drinking water in cities of the Global South have mainly focused on increasing coverage, while water quality has often been overlooked. This paper focuses on drinking water quality in the centralized water supply network of Lilongwe, the capital of Malawi. It shows how microbial contamination of drinking water is unequally distributed to consumers in low-income (unplanned areas) and higher-income neighbourhoods (planned areas).

New technologies for DNA analysis--a review of the READNA Project.

The REvolutionary Approaches and Devices for Nucleic Acid analysis (READNA) project received funding from the European Commission for 41/2 years. The objectives of the project revolved around technological developments in nucleic acid analysis. The project partners have discovered, created and developed a huge body of insights into nucleic acid analysis, ranging from improvements and implementation of current technologies to the most promising sequencing technologies that constitute a 3(rd) and 4(th) generation of sequencing methods with nanopores and in situ sequencing, respectively.

An ecological economic assessment of flow regimes in a hydropower dominated river basin: the case of the lower Zambezi River, Mozambique.

The flow regime of rivers, being an integral part of aquatic ecosystems, provides many important services benefiting humans in catchments. Past water resource developments characterized by river embankments and dams, however, were often dominated by one (or few) economic use(s) of water. This results in a dramatically changed flow regime negatively affecting the provision of other ecosystem services sustained by the river flow.

Improving DNA capture on microarrays by integrated repeated denaturing.

Hybridization of nucleic acids to microarrays is a crucial step for several biological and biomedical applications. However, the poor efficiency and resulting long incubation times are major drawbacks. In addition to diffusion limitation, back hybridization to complementary strands in solution is shown to be an important cause of the low efficiency.

Using a priori knowledge to align sequencing reads to their exact genomic position.

The use of a priori knowledge in the alignment of targeted sequencing data is investigated using computational experiments. Adapting a Needleman-Wunsch algorithm to incorporate the genomic position information from the targeted capture, we demonstrate that alignment can be done to just the target region of interest. When in addition use is made of direct string comparison, an improvement of up to a factor of 8 in alignment speed compared to the fastest conventional aligner (Bowtie) is obtained.

Targeted enrichment of genomic DNA regions for next-generation sequencing.

In this review, we discuss the latest targeted enrichment methods and aspects of their utilization along with second-generation sequencing for complex genome analysis. In doing so, we provide an overview of issues involved in detecting genetic variation, for which targeted enrichment has become a powerful tool. We explain how targeted enrichment for next-generation sequencing has made great progress in terms of methodology, ease of use and applicability, but emphasize the remaining challenges such as the lack of even coverage across targeted regions.

Accurate SNP and mutation detection by targeted custom microarray-based genomic enrichment of short-fragment sequencing libraries.

Microarray-based enrichment of selected genomic loci is a powerful method for genome complexity reduction for next-generation sequencing. Since the vast majority of exons in vertebrate genomes are smaller than 150 nt, we explored the use of short fragment libraries (85-110 bp) to achieve higher enrichment specificity by reducing carryover and adverse effects of flanking intronic sequences. High enrichment specificity (60-75%) was obtained with a relative even base coverage.

Integrating wastewater reuse in water resources management for hotels in arid coastal regions - Case Study of Sharm El Sheikh, Egypt.

Hotels in arid coastal areas use mainly desalinated water (using reverse osmosis) for their domestic water supply, and treated wastewater for irrigating green areas. Private water companies supply these hotels with their potable and non-potable water needs. There is normally a contractual agreement stating a minimum amount of water that has to be supplied by the water company and that the hotel management has to pay for regardless of its actual consumption ("contracted-for water supply").

The effect of temperature and dot size on the spectral properties of colloidal InP/ZnS core-shell quantum dots.

Visual color changes between 300 and 510 K were observed in the photoluminescence (PL) of colloidal InP/ZnS core-shell nanocrystals. A subsequent study of PL spectra in the range 2-510 K and fitting the temperature dependent line shift and line width to theoretical models show that the dominant (dephasing) interaction is due to scattering by acoustic phonons of about 23 meV. Low temperature photoluminescence excitation measurements show that the excitonic band gap depends approximately inversely linearly on the quantum dot size d, which is distinctly weaker than the dependence predicted by current theories.