Photocatalytic activity of layered MoS in the reductive degradation of bromophenol blue.

Molybdenum disulphide (MoS) is a layered material with interesting photocatalytic properties. In this study, a layered MoS was produced using a hydrothermal method. The obtained material was characterised by XRD (X-ray diffraction), XPS (X-ray photoelectron spectroscopy), SEM (scanning electron microscopy), UV-Vis spectroscopy, DLS (dynamic light scattering), and zeta potential analysis.

Determination of Magnetic Parameters of Maghemite (γ-FeO) Core-Shell Nanoparticles from Nonlinear Magnetic Susceptibility Measurements.

Method of determining of magnetic moment and size from measurements of dependence of the nonlinear magnetic susceptibility upon magnetic field is proposed, substantiated and tested for superparamagnetic nanoparticles (SPNP) of the "magnetic core-polymer shell" type which are widely used in biomedical technologies. The model of the induction response of the SPNP ensemble on the combined action of the magnetic harmonic excitation field and permanent bias field is built, and the analysis of possible ways to determine the magnetic moment and size of the nanoparticles as well as the parameters of the distribution of these variables is performed. Experimental verification of the proposed method was implemented on samples of SPNP with maghemite core in dry form as well as in colloidal systems.

BIOCHEMICAL INDICATORS OF HEPATOTOXICITY IN BLOOD SERUM OF RATS UNDER THE EFFECT OF NOVEL 4-THIAZOLIDINONE DERIVATIVES AND DOXORUBICIN AND THEIR COMPLEXES WITH POLYETHYLENEGLYCOL-CONTAINING NANOSCALE POLYMERIC CARRIER.

The aim of this study was to compare the effect of new synthetic 4-tiazolidinone derivatives (compounds 3882, 3288 and 3833) and doxorubicin (positive control) in free form and in their complexes with synthetic polyethyleneglycol-containing nanoscale polymeric carrier on the biochemical indicators of hepatotoxicity in blood serum of rats. The activity of enzymes considered as the markers of hepatotoxicity, as well as. the concentration of total protein, urea and creatinine were measured in blood serum of rats.

[Study of rat blood serum biochemical indicators of cardiotoxic action of novel antitumor 4-thiazolidinone derivatives and doxorubicin in complexes with polyethylene glycol-containing polymeric carrier in the rat blood serum].

The aim of this study was to measure the activity of enzymes which reflect cardiotoxic action in rats of novel synthetic 4-thiazolidone derivatives--3882, 3288 and 3833 that demonstrated antineoplastic effect in vitro towards 60 lines of human tumor cells tested in the framework of the program of screening new anticancer drugs at the National Cancer Institute (USA). Such action of these compounds was compared with the effect of well known anticancer agent doxorubicin and after conjugation of all above mentioned substances with new polyethylenglycol-containing polymeric comb-like carrier that was synthesized by the authors. Among the biochemical indicators of cardiotoxic action of anticancer agents, activity of the following enzymes in rat blood serum showed to be the most informative: creatine kinase, lactate dehydrogenase, aspartate aminotransferase, and alanine aminotransterase.

[Genetic transformation of the moss Ceratodon purpureus by novel polycationic carriers of DNA].

There is a big progress in application of genetic engineering for improving the biological properties of different organisms. Viral and non-viral carriers are used for delivery of genetic material into target cells. Nanoscale polymeric materials of natural and synthetic origin are the most promising gene delivery agents.

[Antitoxic and antioxidant effects of N-stearoylethanolamin in the content of nanocomposite complex with doxorubicin in organs of mice with Lewis carcinoma].

The aim of the study was to evaluate the possibility to reduce the doxorubicin toxic effects by its immobilization with N-stearoylethanolamine (NSE) on nanocarier polyethylene glycol. The studied parameters of the doxorubicin toxicity were: the level of creatinine in the mice blood plasma and activity of alanine aminotransferase and aspartate aminotransferase in the blood plasma of mice. The activity of catalase superoxide dismutase, glutathione peroxidase and intensity of lipid peroxidation was determined in the tissues of the heart, kidneys and liver.

[Antibiotic activity of some fungi].

Biological activity of pure extracts of cultural filtrates of Aspergillus niveus 2411, Myrothecium cinctum 910, Ulocladium consortiale 960, Penicillium sp. 10-51 concerning wide spectrum of test-organisms was investigated. It was shown that the extracts had high levels of antibacterial activity against Gram-positive microorganisms, especially against Bacillus genus.

[Adjuvant properties of polymer based on acrylic acid].

Adjuvant properties of the polymer containing acrylic acid, glycidyl methacrylate, triethylene glycol methacrylate and butyl acrylate have been established. Antibodies to ovalbumin and bovine serum albumin in the blood of mice were revealed using dot blot analysis and immunoenzyme analysis when applying the investigated polymer as a carrier of these protein antigens. Adjuvant properties of the polymer were compared to aluminum hydroxide, which is a component of many traditional vaccines.

[Functions of metallothioneins and a system of antioxidant defense under the effect of Co- and Zn-containing nanocomposites on crucian carp (Carassius auratus gibelio)].

The effect of metal-nanocomposites (Me-NC) of cobalt and zinc (Co- and Zn-NC, correspondingly) synthecized on the basis of vinylpyrrolidone (PS) on the metal-accumulative proteins with antioxidant potential metallothioneins (MT) in crucian carp (Carassius auratus gibelio) was studied. Fish was subjected to the effect of Co-NC, Zn-NC, Co2+, Zn2+ or polymer carrier (PC) in the concentrations correspondent to 50 microg x Co/l or 100 microg x Zn/l during 14 days. It was shown that the MTs response is highly specific for the nature of metal, both in ion and Me-NC form: the effect of Co and Co-NC provoked the elevation of total MT concentration (MT-SH) and activation of antioxidant defence, whereas Zn and Zn-NC induced the decrease of the concentration of MT-SH and the inhibition of antioxidant defense.

[Effect of free and polymer carrier encapsulated doxorubicin towards HCT116 cells of human colorectal carcinoma].

Development of novel nanoscale functionalized carriers is nowadays one of the most urgent problems in cancer treatment. The aim of our study was to compare the antineoplastic effect of free doxorubicin and its complex with a nanoscale polymeric carrier towards HTC116 colorectal carcinoma cells. It was established that application of the complex of poly(5-tret-butylperoxy)-5-methyl-1-hexene-3-in-co-glycydyl metacrylat)-graft-polyethyleneglycol (poly(VEP-GMA-PEG)-graft-PEG), where VEP--5-tret-butylperoxy)-5-methyl-1-hexene-3-in; GMA--glycydyl metacrylat; graft-PEG--graft-polyethyleneglycol accordingly, functionalized with phosphatidylcholine for doxorubicin delivery increased 10 times the efficiency of cytotoxic action of this drug, as compared wich such efficiency in case of the action of free doxorubicin.

[Protective effect of N-stearoylethanolamine in suspension and in nanocomposite complex in the organs of mice with the Lewis carcinoma under doxorubicin intoxication administration].

The antioxidant effects of N-stearoylethanolamine (NSE) in the nanocomplex composition and in suspension are shown on the model of intoxication by doxorubicin in conditions of development of the Lewis carcinoma in the heart, kidneys and liver tissue and in the blood plasma of female mice. The NSE suspension reduces the level of urea in the blood plasma of mice with the Lewis carcinoma, which growth was revealed as a result of introduction of doxorubicin. Under introduction of nanocomplex the amount of urea remains at the level of that in the intact mice.

[Evaluation of biological effects of cobalt-nanocomposites with the use of biochemical markers of bivalve mollusk Anodonta cygnea].

Intensive implementation of nanomaterials requires development of novel methods for evaluation of their potential ecotoxicity. The aim of our study was to identify specific characteristics of the effect of cobalt-nanocomposite (Co-NC) on the molecular stress-responsive system in the digestive gland of bivalve mollusk Anodonta cygnea. Nanocomposite was synthesized by mixing alcohol solution of copolymer N-vinylpirrolidone, 5-(tret-butylperoxy)-5-methyl-1-hexene-3-yne and dimethylaminoethylmetacrylate and cobalt (II) chloride.

[Evaluation of micromycetes potential concerning synthesis of biologically active substances].

Screening of 41 cultures of some researched strains of micromycetes by their antibiotic and phytotoxic activities in relation to different test-organisms--Gram-positive, Gram-negative, phytopathogenic bacteria, yeasts and green algae was conducted. The wide spectrum of antibiotic action was characterized for the cultures of Gliocladium catenulatum 2709, 3942 Penicillium vulpinum 3957, Ulocladium atrum 1889, U.consortiale 960, Trithirachium album 2673.

[Biosynthesis of macrocyclic trichothecenes: some aspects of physiology].

The data of long-term investigations of the control of macrocyclic trichothecenes biosynthesis by means ofthe elements of mineral nutrition, microelements in particular in Dendrodochium toxicum Pidopl. et Bilai are presented. The media for providing the dominant synthesis (50-70 %) of certain component from the composite complex of dendrodochins and so for obtaining the fractions enriched with this component, have been worked out.

[Effect of heavy metal mixture on the main functions of the central nervous system].

The influence of heavy metals salts mix, the most widespread pollutants of Prydnieprovsky region natural reservoirs on animal behavioral reactions, memory processes and total bioelectric activity of various brain departments has been investigated. It was revealed that heavy metals consumption with drinking water results in the development of the neurotization processes, at that due to animals hyperactivity there is an exhaustion of nervous centers that is reflected by prolongation of immobility periods in animals. Increase of the latent period of conditioned reflex formation in the 8th radial labyrinth together with decrease of task fulfillment efficiency indicate deterioration of the training ability of these animals and impairment of their short-term memory.

[Effect of ecopathogenic environmental factors on the system of antioxidant defense in different parts of the rat brain].

Influence ofecopathogenic environmental (radiation-chemical) factors on activities of superoxiddismutase, catalase and total antioxidative activity (TAA) has been investigated in morphologically and functionally different parts of rat brain. It has been found that reaction of cells in brain cortex, subcortical structures and cerebellum to these factors in all cases is revealed through the antioxidant enzyme activities decrease in all studied brain structures but in various degrees. In brain cortex, which is characterized by higher metabolic level, functional complexity and specialization, the higher level of antioxidation defense is marked both in normal state and under the action of negative factors, that allows to maintain the prooxidation-antioxidation balance.

[Antibiotic and phytotoxic properties of some Aspergillus parvulus Smith strains].

Antibiotic activity screening of 15 species of Aspergillus parvulus were made relative to different test-organisms (gram positive, gram negative, phytopathogenic bacteria and yeasts) and phytotoxic activity screening relative to different algae. The researched strains 3387, 340, 3142, 1813 of A. parvulus had a wide spectrum of antibiotic activity and high phytotoxic activity.

[Antibiotic properties of some species of genus Aspergillus MICH].

Antibiotic activity screening of some species of genus Aspergillus (64 strains) to different test-organisms (gram positive, gram negative, phytopathogenic bacteria and yeasts) has been carried out. The researched strains A. parvulus, A.

[Use of cyanobacteria for indication of toxic action of stachybotrys toxins].

Screening of cyanobacteria cultures for their sensitivity to the action of complex preparations of toxins from 18 strains of Stachybotrys chartarum has been carried out with the purpose to search for new test-organisms to create a reliable system of biotesting of stachibotryotoxines. In contrast to other cultures of unicell (Anacystis nidulans) and filamentous (Anabaena sp., Anabaena variabilis, Nostoc linckia, Plectonema boryanum) cyanobacteria used in the experiment, only one culture of unicell cyanobacterium Synechococcus cedrorum proved to be highly sensitive to the action of preparations of the whole group of studied mycotoxins.

Certain peculiarities of biological action of the stachybotryotoxin preparations.

Some biological and toxigenic properties of 47 Stachybotrys alternans strains, isolated from different ecological niches have been studied. On the basis of antifungal, phytotoxic and dermacidic properties the strains studied can be divided into several characteristic groups: strains with clearly expressed dermacidic reaction and without other properties studied (19.1%); strains displayed only antifungal activity to a number of yeast test cultures (4.

Comparative characteristics of some cultural and toxigenic properties of the Dendrodochium Bonorden and Myrothecium Tode ex Fr. representatives.

The comparative study of sporulation intensity, change of medium pH, yield of biomass and ethanol soluble fraction (ESF) of extracellular metabolites as well as antibiotic and toxigenic properties of 106 fungi strains which were the potential producers of macrocyclic trichothecenes has been carried out under the conditions of stationary cultivation. It was shown that all the studied Dendrodochium strains were characterized by moderate growth, variations of biomass were within 3.5-4.

[Blood coagulating properties of the copperhead Agkisrodon halys halys venom and its thrombin-like fractions].

Venom and its fraction with hemocoagulation properties from Agkistrodon halys halys exhibited dissimilar effects on blood plasma and fibrinogen preparation. The major component of this fraction proved to be a thrombin-like enzyme, properties of which were studied by means of several coagulation tests. Dissimilarity in coagulating effects of the venom and its thrombin-like fraction as well as different physical properties of blood clots formed after their action suggest that the venom from Agkistrodon halys halys contained other protein factors affecting blood coagulation besides the thrombin-like proteases.