[Properties of the replicator region of the natural plasmid pLG13 containing genes of the EcoRV restriction-modification system].

The previously constructed plasmid pILRV8 that induces endonuclease EcoRV gene overexpression kills cells of some E. coli strains under the induction of this enzyme synthesis. Cell transformation by natural plasmid pLG13 carrying genes of the EcoRV restriction--modification system was found to appreciably enhance cell viability ("survival") under endonuclease overproduction.

[Criteria of selection of patients for organ-sparing operations in tubal pregnancy].

Rabbit experiments indicated that resection of a half of the oviduct anastomosed failed to impair its function and reproduction of viable progeny. With greatly varying diameters of sutured pieces, the valuable anastomosis was made by additional cuts of the narrow end of the oviduct. Analysis of 332 case histories and morphological studies of 10 control and 70 pregnant tubes suggested criteria for selection of patients, for organ-preserving surgeries.

[Cloning and regulation of gene expression of EcoRV restriction- modification system].

A number of recombinant plasmids, containing EcoRV restriction-modification genes have been constructed. Individual genes of this system were introduced into plasmids of various incompatibility groups. Promoter regions of genes encoding methylase and restrictase have been cloned and studied.

[A new highly active proteolytic myelin enzyme and its possible role in the mechanism of the demyelinating process].

The study of the myelin in the rabbit brain and in biological fluids of patients with multiple sclerosis has revealed the presence of a new highly active proteolytic enzyme. The article describes the physicochemical properties of this enzyme. Its role in the pathogenesis of the demyelinizing process is emphasized: normally being coupled with an inhibitor the enzyme is released as a result of the demyelinizing process and becomes active.

[Frequency of structural chromosome aberrations in the hepatocytes of rats exposed to polymeric 239Pu].

Intravenous injection of polymeric 239Pu(IV) nitrate (166.5, 55.5 and 18.

[Frequency of chromosome aberration in liver cells of rats of various ages].

Frequency of chromosome aberrations in the liver cells of hepatectomized Wistar rats aged 3, 9, 12 and 18 months was studied. Changes in the number of chromosomes and damage of their structure at the metaphase stage of the first division hepatocytes are analyzed. The content of aneuploid cells was 1-3% of the total number of metaphases studied and did not change with the age of animals.

[Study of the synovial fluid in rheumatoid arthritis].

A comparative study of the synovial fluid (SF) composition was conducted in 72 patients with rheumatoid arthritis (RA) with relation to the presence of the rheumatoid factor (RF) and clinical manifestations of this disease. A statistically significant difference in the SF composition (the number of leukocytes, neutrophils and ragocytes) was shown in seropositive and seronegative RA. The content of total protein and glucose in SF did not practically differ in both cases.

[Study of articular cartilage and the synovial membrane using IR spectroscopy].

The infrared spectra of normal knee joint cartilage, normal and rheumatoid arthritis-affected human synovial membrane and the same normal bovine tissues were obtained over the region of 400--4000 cm-1. A comparative analysis of the spectra of these tissues and those containing hyaluronate, protein-chondroitin-keratan sulfate aggregates of cartilage proteoglycans and heparin made it possible to identify greater absorption bands of these biopolymers in the tissue spectra. The interpretation of the results obtained is presented.

[Etiological and immunopathological markers in hepatitis B patients in the early convalescence stage during the development of chronic liver involvement].

Etio- and immunopathological markers in hepatitis B are analysed. Combinations of specific HB markers in a highly aggressive process are characterized in comparison with the lack of this aggressiveness. Among immunopathological markers, most informative were the levels of C3 component of complement and of immunocomplexes from the point of view of acute hepatitis B prognosis.

[Attitude of hysterical patients to an experimental situation].

The article analyses the way in which hysterical patients' attitude to an experimental situation affects the results of an experiment. It has been assumed that depending on the nature of an experiment and the motivational content of the stimulating material the patients' attitude toward the experiment will also change. Comparison of the above attitudes in different variants of technique denoted as "the level of claims" has made it possible to characterize the specificity of hysterical patients' motivation expressed in excessively high claims and maintenance of inadequate self-assessment.

Examination of IgA1-protease activity in Bordetella.

A total of 43 strains of bacterial genus Bordetella was studied as to the possible extracellular enzyme production responsible for Al-immunoglobulin cleavage. This was observed to be cleaved by 9 of 21 controlled strains of B. pertussis, one of 15 strains of B.

[Rapid method of determining the dry mass of whooping cough microbes].

A colorimetric method for the rapid determination of the quantitative content of microbial mass in B. pertussis suspensions has been developed. The method is based on the indirect determination of carbon in microbial suspensions by its oxidation with the mixture consisting of potassium bichromate in concentrated sulfuric acid and the subsequent colorimetric analysis of the products of this reaction.

Adaptation to laboratory and wild animals of the haemorrhagic fever with renal syndrome virus present in the foci of European U.S.S.R. Brief report.

Four strains of haemorrhagic fever with renal syndrome virus (HFRSV) from rodents or patients in European U.S.S.

[Demonstration of the virus of hemorrhagic fever with renal syndrome in the organs of rodents from natural foci of the infection in the USSR and the serodiagnosis of the diseases].

An indirect fluorescent antibody test (IFAT) and an enzyme-immunological test (ELISA) were used for the detection of HFRS virus in organs of rodents from HFRS foci in the USSR. The virus was found in 115 out of 1120 bank voles, 9 out of 92 redbacked voles, and 2 field voles examined. Spontaneous infection-rate of bank voles in population varied from 1.

The results of study of pertussis component of DPT vaccine produced in USSR and some other countries.

The paper presents the results of testing of 16 vaccines under the letter code from 8 countries received from WHO. These vaccines differed both in toxicity and potency. Six lots contained from 1.

Detection of hemorrhagic fever with renal syndrome (HFRS) virus in the lungs of bank voles Clethrionomys glareolus and redbacked voles Clethrionomys rutilus trapped in HFRS foci in the European part of U.S.S.R., and serodiagnosis of this infection in man.

The antigen of HFRS virus was demonstrated by means of the indirect fluorescent antibody procedure in the lung tissue of bank and redbacked voles (Clethrionomys glareolus, Cl. rutilus) trapped in HFRS foci in the European part of USSR. This antigen has been used satisfactorily for serodiagnosis of HFRS in several European and Asian regions of the USSR where HFRS had been found to be endemic.

[New data on glycosaminoglycans and proteoglycans].

The most important investigations on glycose aminoglycans and proteoglycans, carried out within the recent 5-7 years, are reviewed. Data on primary structures repeating in glycose aminoglycans and on relationship between these biopolymers and protein components in proteoglycans are considered. Newer information on conformational characteristics of glycose aminoglycans, various complexes aggregation of proteoglycans and distribution of the biopolymers in animal tissues and cells is discussed.