Quantitative Rapid Magnetic Immunoassay for Sensitive Toxin Detection in Food: Non-Covalent Functionalization of Nanolabels vs. Covalent Immobilization.

In this study, we present a novel and ultrasensitive magnetic lateral flow immunoassay (LFIA) tailored for the precise detection of zearalenone, a mycotoxin with significant implications for human and animal health. A versatile and straightforward method for creating non-covalent magnetic labels is proposed and comprehensively compared with a covalent immobilization strategy. We employ the magnetic particle quantification (MPQ) technique for precise detection of the labels and characterization of their functionality, including measuring the antibody sorption density on the particle surface.

[A new rapid automated photometric method in analysis of quantitative indicators of food product contamination].

Thirty-five specimens of pasteurized milk were tested for bacterial contamination by rapid autocalibration photometry for liquid, semiliquid, and homogenized hard foodstuffs, developed by the Ecotest Firm, Russia. Photometric (turbidimetric) analysis was carried out using the Labsystems (Finland) iEMS Reader plate photometer and Russian Analitika BACT software. The results of traditional analysis and the new rapid method developed by Ecotest were in good correlation, the differences did not surpass the magnitude of errors of measurements.

[Work capacity of the operators of chemical industry and its effect on motor activity].

Motor activity, energy metabolism, work productivity, functioning and regulation of cardiovascular system were examined in machine operators working at 2 chemical enterprises. The energy loss within the shift appeared to vary from 800 to 1200 kcal, that within the day--from 2,500 to 2,900 kcal. The examinees demonstrate lack of physical training that affects the functioning of cardiovascular system.

[The problems and outlook for delivery in pregnant women with uterine scars following cesarean section].

Twenty-seven pregnant women with cicatrices on the uterus after cesarean section had US-monitored spontaneous deliveries with calypsol analgesia (the drug was permanently i.v. infused).

[Dynamics and mental disorders in patients with portal hypertension after surgical treatment].

On the basis of the clinical, experimental-psychological and electroencephalographic examinations of 173 patients with portal hypertension (intrahepatic--110 and extrahepatic--63) the authors elucidated the regularities of the formation of mental disorders, their clinical characteristics and the course following surgical treatment. The asthenic syndrome with marked vegetative disturbances was the most prominent feature in the clinical picture. Patients with hepatic cirrhosis were characterized by a more severe asthenia and greater intensity of vegetative symptomatology as compared to patients with an extrahepatic form of portal hypertension.

[Effect of technical threonine sources on homoserine biosynthesis by mutant Brevibacteruim flavum 2T].

The effect of threonine technical sources on the homoserine biosynthesis by the threonine auxotroph Brevibacterium flavum 2T when cultivated on sucrose and acetic acid containing media was investigated. Various threonine sources (corn extract and fodder yeast, microbial biomass and soybean meal hydrolyzates) prepared by means of different hydrolyzing agents (acids, enzymes, autolysis) were used. The most effective substrate was protein--vitamin concentrate hydrolyzate, particularly combined with corn extract in the ratio 1: 0,25-0.

[Effect of the carbon source and aeration conditions on homoserine lysine biosynthesis in the threonine-dependent mutant Brevibacterium flavum 2T].

The effect of two carbon sources (sucrose and acetate), aeration conditions and threonine concentration on the homoserine and lysine biosynthesis by the threonine-dependent mutant Brevibacterium flavum 2T was examined. It was demonstrated that acetate provided the predominant synthesis of homoserine to a far greater extent than sucrose (with the weight/weight ratio of homoserine : lysine being 2.5-5.

[Regulation of lysine biosynthesis in the leucine-dependent mutant of Micrococcus glutamicus].

The role of leucine in metabolism of Micrococcus glutamicus was examined in relation to the lysis synthesis by the homoserine- and leucine-dependent strains of M. glutamicus 106 and the homoserine-dependent strain of M. glutamicus 95.

[Effect of aeration on lysine biosynthesis in nutrient media with different concentrations of components].

The lysine synthesis by the culture M. glutamicus T-3 on nutrient media with varying molasses concentrations was studied during cultivation under different aeration conditions. With an increase in the nutrient concentration the relationship between the lysine yield and aeration rate became very manifest.

[Some properties of beta-aspartate kinase from Micrococcus glutamicus-95 bacteria].

Kinetic and allosteric properties of beta-asparatatekinase from Micrococcus glutamicus-95 were studied. Coarse protein fraction, sedimented at 0.8 from saturated (NH4)2SO4, was used as an enzyme preparation.