Structurally diverse sesquiterpenoids from Sarcandra glabra and their anti-inflammatory activities.

Fifteen sesquiterpenoids, including five previously undescribed monomers with oxidative rearranged skeletons (sarglabenoids A-E, 1-5) and three previously unreported lindenane [2 + 2] dimers (sarglabenoids F-H, 6-8), alongside seven related precursors (9-15), were isolated from the root of Sarcandra glabra. The structures of these compounds were elucidated using a combination of high-resolution electrospray ionization mass spectrometry, one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy, the circular dichroism exciton chirality method, electronic circular dichroism, and nuclear magnetic resonance calculations integrated with DP4+ analysis. Compounds 1 and 2 feature an unique 5/5 spiro ring system, which is likely derived from a pinacol rearrangement of precursor 14.

Influence of cell shape on sonoporation efficiency in microbubble-facilitated delivery using micropatterned cell arrays.

Microbubble-facilitated sonoporation is a rapid, versatile, and non-viral intracellular delivery technique with potential for clinical and ex vivo cell engineering applications. We developed a micropatterning-based approach to investigate the impact of cell shape on sonoporation efficacy. Cationic microbubbles were employed to enhance sonoporation by binding to the cell membrane electrostatically.

Effects of Culture Medium Enrichment with Zinc on Astaxanthin Accumulation in a New Strain of the Microalga .

High Zn concentrations in microalgal cells that produce astaxanthin as a feed additive can reduce the symptoms of malnutrition in aquatic animals. Therefore, in this study, we analysed the effect of Zn in the culture medium on the growth of a newly isolated microalgal strain ZY24. Zn and white light stress altered the pigment content in microalgal cells.

Microbial production of methyl-uranium via the Wood-Ljungdahl pathway.

The misuse of uranium is a major threat to human health and the environment. In microbial ecosystems, microbes deploy various strategies to cope with uranium-induced stress. However, the exact ecological strategies and mechanisms underlying uranium tolerance in microbes remain unclear.

The value of electroencephalography features in the prognostic evaluation of large hemispheric infarction patients at different time intervals.

Background: Large Hemispheric Infarction (LHI) is a devastating disease with high mortality. This study aimed to use electroencephalography (EEG) to evaluate the death risk of LHI patients and identify suitable evaluation time.

Methods: This study retrospectively collected clinical and EEG data from 73 LHI patients, dividing them into death and survival group at discharge.