Production and purification of ovine anti-tetanus antibody.

We used the ovine as bioreactor for the production and optimization of anti-tetanus toxin antibody. Four female sheep were immunized with human tetanus vaccine (TT-alum) every two weeks for 16 weeks, after which serum was collected and its titer was estimated by ELISA. The highest titer obtained was 39,000 IU ml-1.

Soybean and ascorbate feeding in experimental carcinogenesis: immunological studies.

Soybean and/or sodium ascorbate have been demonstrated to have protective effects against carcinogenicity of the nitrosamine precursors dibutylamine and sodium nitrite in rats. Some immunological aspects of the cellular and humoral compartments of the immune system were investigated in this experimental model. The parameters chosen for the study were the lymphocyte transformation test, migration inhibition test, the demonstration of Fc and Fc plus complement receptors on the lymphocyte surface (EA and EAC rosette-forming test), and the determination of total serum proteins and their fractions.

Inhibition of lactate dehydrogenase isoenzyme associated with anaerobic respiration in schistosomiasis intermediate host snails.

Lactate dehydrogenase isoenzyme (LD5) which is associated with anaerobic respiration was inhibited to a certain degree in Biomphalaria alexandrina snails, the intermediate host for Schistosoma mansoni. Urea and thiourea were used as inhibitors. The effect of LD5 inhibition on the mortality rate of infected Biomphalaria alexandrina snails and on the susceptibility of the snails to the trematode infection was also studied.

Kinetic properties of two transaminases and lactate dehydrogenase of Biomphalaria alexandrina snails, intermediate hosts of Schistosoma mansoni.

Aspartate (AST) and alanine (ALT) aminotransferase together with lactate dehydrogenase (LD) from the tissue homogenate of the Biomphalaria alexandrina snails, were partially characterized by measuring the Michaelis constant (km) and the maximum velocity (Vmax). The isoenzymatic pattern of lactate dehydrogenase was investigated through polyacrylamide gel electrophoresis.

Measurement of some selected enzymatic activities in infected Biomphalaria alexandrina snails.

The activities of aspartate (AST) and alanine (ALT) aminotransferases and that of lactate dehydrogenase (LD) were measured in the homogenate of infected Biomphalaria alexandrina snails, the specific intermediate hosts for the parasite Schistosoma mansoni which is the cause of the disease schistosomiasis. The isoenzymatic pattern of LD was also studied in the infected snails tissue.