CORRELATION OF THYROID AUTOIMMUNITY WITH ATHEROSCLEROSIS EVALUATION IN HASHIMOTO'S THYROIDITIS.

The relationship between subclinical hypothyroidism (SH) and Atherosclerotic (At) cardiovascular diseases (CVD) has been one of the most popular topics but causal connection between Hashimoto thyroiditis (HT), lipid profile and follicular epithelial molecular biology is controversial. We investigated 3 groups of patients (group I - HT, group II - HT+At, group III - At). All laboratory tests for thyroid function and lipid profile detection were used according to international guideline recommendations, coronary and femoral arteries intima-media thickness (IMT) were tested by high-resolution ultrasonography, thyroid gland histology and immunohistochemistry carried out by p63 and S100 protein expression control.

[HISTOLOGICAL AND IMMUNOHISTOCHEMICAL PROFILE OF THE MYOCARDIUM DURING ALCOHOL INTOXICATION IN THE EXPERIMENT].

The purpose of this study was to identify early structural and conjugated with them molecular changes in the myocardium, stroma and blood vessels under experimental alcohol intoxication, with histological and immunohistological methods. The experiments were carried out on white male rats with initial body weight 180-200gg. Accordingly, specific tasks were carried out 2 series of experiments: I series - acute alcohol intoxication, II series - chronic alcohol intoxication.

HURTLE CELLS IMMUNOHISTOCHEMICAL ACTIVITIES IN HASHIMOTO THYROIDITIS PARENCHYMA.

The present study was designed to evaluate the participation and utility of Hǘrtle cells morphological requirment and transformation under Hashimoto autoimmune thyroiditis versus Riedel´s struma. Several markers have been evaluated to detect induced activities of Hǘrtle cells. Study subject - specimens (tissue fragments) collected from TG surgery (thyroidectomy) for mollecular (receptor) diagnosis of Hǘrtle cells activities using routine histological and immunohistochemical samples.

HISTOLOGICAL AND IMMUNOHISTOCHEMICAL STUDIES OF ATHEROSCLEROTIC AND SENILE CALCIFIC AORTIC VALVE STENOSIS.

THE GOAL OF THIS STUDY: description of morphogenesis aortic valve stenosis in the senile and vice atherosclerotic aortic valve and definition inflammatory processes in them, improving pathology-anatomic diagnostic, macro- and microscopic examination of the stage of development of atherosclerotic plaques in the operating material fragments of aortic valve on the basis of morphological (histochemical and immunohistochemical studies by using markers CD31, VEGF) and idiopathic characteristics of atherosclerotic plaques, the differential diagnosis of the degree of calcification of the aortic valve in the atherogenic and idiopathic aortic stenosis. Calcification in senile aortic stenosis is characterized by three main parameters: infiltration of inflammatory cells, while senile origin stenosis observed the potential growth of angiogenesis. In endothelial walls of the aortic valve imunnhistochemicaly revealed high expression of endothelial and panendothelial growth factors.

[Adipocytes and macrophages functional morphology in obese humans: correlation with plasma leptins level].

White adipose tissue macrophages and both in peripheral blood simples obtained in biopsies from male (n=14) and female (n=11) gluteal depots, were observes by light and electron microscopic methods, in obese in patients with BMI (35,2±7,9 and 31,3±8,5) in female and men, consequently. The data indicates on the positive correlation between adipocytes death, macrophages local activization and leptins level in obese individuals. Recent evidence indicates the correlation between increased leptin prodaction level and adipocytes necrotic foci in WAT, which is trigger lipid tissue persistans necrotic foci and formation chronic inflammation response in obese individuals.