DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual automated methods, using four commercially kits.

Background: Nucleic acid isolation from formalin-fixed, paraffin-embedded tissue (FFPET) samples is a daily routine in molecular pathology laboratories, but extraction from FFPET is not always easily achieved. Choosing the right extraction technique is key for further examinations.

Aim: To compare the performance of four commercially available kits used for DNA extraction in routine practice.

[Influence of HPV infection on cell cycle regulation in epithelial cell alterations of cervix uteri: Immunohistochemical and in situ hybridization observations].

The HPV16 E6 DNA incorporation into the cervical epithelial cell genome was determined. In addition, using p16, p21 and p27 protein immunohistochemistry, we intended to present how the normal cell cycle machinery was disturbed in cervical epithelial cells. In CIN1 the epithelial cells are transformed into koilocytes, p16 is not expressed.

[Colorectal cancer susceptibility genetic variants in tumor free and colorectal carcinoma bearing Hungarian population. Individual predisposition to cancer].

Genome-wide association studies (GWAS) using population-based designs have identified many genetic loci, at which common variants can influence the risk of developing the sporadic colon cancers. These are single nucleotide polymorphisms (SNPs) located on different chromosomes, close to genes involved in cancer developing process, and the SNPs modify their functions, and as a consequence the cancer risk is increased. Our aim was to provide frequency distributions data of variable (risk) allele of six independent SNPs in patients with colorectal cancers and in control Hungarian population, predicting the increased risk effect of sequence variant of SNPs.