Nuclear peptides from calf liver: large scale isolation and fractionation; control of gene expression in cell-free systems, and inhibition of growth of cells in culture.

DNA and nuclear RNA fractions contain small peptides (mol. wt. 600-1500) attached noncovalently.

Nuclear peptides from calf liver: large scale isolation and fractionation; control of gene expression in cell-free systems, and inhibition of growth of cells in culture.

DNA and nuclear RNA fractions contain small peptides (mol. wt. 600 - 1500) attached noncovalently.

Nuclear deprimerones (low molecular weight peptides controlling gene expression) are associated with DNA and nuclear RNA.

Isolated, deproteinized nucleic acids from calf thymus and rat liver nuclei were fractionated on Bio-Gel A-5m into DNA and RNA fractions. Extraction of deproteinized DNA and nuclear RNA with 80% ethanol at pH 9.5 yielded low molecular weight peptides (deprimerones) capable of inhibiting DNA transcription in a cell-free system.

Poly(A)-mRNA deprimerones in rat liver and Novikoff hepatoma cells.

The poly (A)-mRNA fraction isolated by chloroform deproteinization of liver polysomes and poly(U)-Sepharose chromatography contains a low molecular weights (congruent to 1000) peptidic fraction. The peptides which we suggested to call deprimerones (1) were extracted with 80% ethanol at pH 9.5; after ethanol evaporation, they were purified on Sephadex G-25 column as a fraction of mol.

Antineoplaston A in cancer therapy. (I).

Twenty-one patients with advanced cancer or leukemia were treated with antineoplaston A and followed for up to nine months. Dosage by intravenous, intramuscular, subcutaneous, rectal, intrapleural, intravesical and/or topical administration ranged from 0.6 to 33 U/m2/24 h.