Single-cell transcriptome atlas of peripheral immune features to Omicron breakthrough infection under booster vaccination strategies.

Introduction: The high percentage of Omicron breakthrough infection in vaccinees is an emerging problem, of which we have a limited understanding of the phenomenon.

Methods: We performed single-cell transcriptome coupled with T-cell/B-cell receptor (TCR/BCR) sequencing in 15 peripheral blood mononuclear cell (PBMC) samples from Omicron infection and naïve with booster vaccination.

Results: We found that after breakthrough infection, multiple cell clusters showed activation of the type I IFN pathway and widespread expression of Interferon-stimulated genes (ISGs); T and B lymphocytes exhibited antiviral and proinflammatory-related differentiation features with pseudo-time trajectories; and large TCR clonal expansions were concentrated in effector CD8 T cells, and clonal expansions of BCRs showed a preference for IGHV3.

TLR7/8/9 agonists and low-dose cisplatin synergistically promotes tertiary lymphatic structure formation and antitumor immunity.

In situ vaccination (ISV) triggers antitumor immune responses using the patient's own cancer antigens, yet limited neoantigen release hampers its efficacy. Our novel combination therapy involves low-dose local cisplatin followed by ISV with a TLR7/8/9 agonist formulation (CR108), in which CR108 boosts and sustains the antitumor responses induced by the cisplatin-released neoantigens. In mouse models, the cisplatin+CR108 combination significantly outperformed cisplatin or CR108 alone in abrogating established 4T1 and B16 tumors.

Association of Organophosphorus Pesticide and Glyphosate Exposure with Nasal Flora and Total IgE in Solar Greenhouse Workers: A Unique Farmer Group.

Studies have shown that the presence of allergens, including insecticides, significantly increases the risk of occupational allergic diseases among solar greenhouse workers. However, no studies have yet investigated the relationship between organophosphorus pesticide use by greenhouse workers and allergic diseases, and the role of the flora in this context remains unclear. Therefore, this study aimed to investigate the relationship between combined exposure to organophosphorus pesticides (OPs) and Glyphosate (GLY) and changes in total immunoglobulin E (IgE) levels, as well as to analyze the role of nasal flora in allergic status.

In Situ Infared Optical Fiber Sensor Monitoring Reactants and Products Changes during Photocatalytic Reaction.

An in situ monitoring reaction can better obtain the variations during the progression of the photocatalytic reaction. However, the complexity of the apparatus and the limited applicability of substances are the common challenges faced by most in situ monitoring methods. Here, we invented an in situ infrared optical fiber sensor to monitor the reactants and products during photocatalytic reaction.

Serum Cystatin S (CST4): A Novel Prognostic Marker for Gastric Cancer.

Background: Serum Cystatin S (CST4), a secretory protein that inhibits cellular matrix degradation, significantly influences the tumor microenvironment and tumor progression. However, the prognostic value of serum CST4 in gastric cancer (GC) remains unclear. This study aims to explore serum CST4's utility in GC prognostic assessment.