Double labeling of SIgG+ cells harboring a lymphotropic herpesvirus by avidin-biotin complex immunoperoxidase and immunogold-silver staining techniques.

By reversing the usual order of double-staining procedures, we obtained optimal conditions for simultaneous detection of guinea pig lymphotropic herpesvirus (GPHLV) and surface immunoglobulin G-positive (SIgG+) cells in paraffin-embedded tissue sections. Of the different fixatives tested, Bouin's solution gave the best preservation of morphology and cell surface IgG. Double immunolabeling was best achieved when avidin-biotin complex immunoperoxidase (ABC-IP) staining was performed first for detection of intracellular viral antigen, followed by immunogold-silver staining (IGSS) for localization of cell surface IgG.

Effect of cyclosporin A immunosuppression on primary lymphotropic herpesvirus infection in the guinea pig.

Female guinea pigs pretreated for 2 days with cyclosporin A (CsA), were then inoculated intranasally (IN) or intraperitoneally (IP) with a lymphotropic herpesvirus (GPHLV) and followed by 4 additional daily doses of CsA. Immunosuppressed animals did not show lymphocytosis and virus infectivity titers in their spleen, cervical lymph node and blood mononuclear cells were lower than those of oil-treated controls. While IN-inoculated CsA-treated animals expressed higher virus infectivity titer in their lungs compared to oil-treated controls, this difference was not seen in the IP-inoculated groups.