The role of iron in metal-metal interactions in hard tissues of roe deer (Capreolus capreolus L.).

Introduction: The toxicity of heavy metals in humans and animals has been attributed to their potential for accumulation in solid tissues and interference with other vitally important elements present in the body. We have devised this study to determine the content of some metals in bone and to determine with the Statistica Neural Networks program whether the content of one metal has any effect on the content of other metals.

Material And Methods: Our material consisted of mandibles obtained from deer aged 2 to 6 years.

Characterisation of rat and human tissue alkaline phosphatase isoforms by high-performance liquid chromatography and agarose gel electrophoresis.

Alkaline phosphatase (ALP) exists as several isoenzymes and many isoforms present in tissues and serum. The objective of this study was to separate tissue ALP forms in rats and humans and characterise their properties. The materials for the investigation were intestinal, bone, and liver tissue of rats and commercially available human preparations of tissue ALP.

Shell of snail Helix aspersa maxima (Helicidae) as a protection of bioaccumulation toxic sodium fluoride in soft tissue.

The aim of this work was to determine the extent of bioaccumulation of sodium fluorides in tissues of snails under strictly controlled conditions, and also to determine resistance and tolerance to sodium fluoride load in these organisms. The study was performed on snails removed from aestivation. Quantitation of fluoride levels was done in soft tissues (foot, hepatopancreas) and shells of mature snails.

Guanine and inosine nucleotides, nucleosides and oxypurines in snail muscles as potential biomarkers of fluoride toxicity.

The aim of the present study was to determine the toxicity of fluorides on energy metabolism in muscles of the Helix aspersa maxima snail. Qualitative and quantitative analysis of purine compounds was performed in slices of foot from mature snails with high-performance liquid chromatography. Fluoride concentrations were measured using an ion-selective electrode and gas chromatography.

[Interactions of aluminofluoride complexes (ALFx) with high-energy compounds (ADP, ATP) as revealed by molecular modeling].

Introduction: Aluminofluoride complexes (AlFx) are toxic substances at the cellular and subcellular level, apparently implicated in degeneration of the nervous system. The present study focused on direct interactions with A1Fx of two common adenine nucleotides: adenosine diphosphate and adenosine triphosphate, both of which play an important role in many biochemical processes. The aim was to search for interactions between ADP-AlIF and ATP-AlFx complexes depending on the environment of the reaction.