Metabolic engineering of for high-level production of pneumocandin B.

Pneumocandin B (PB) is a lipohexapeptide synthesized by and serves as the precursor for the widely used antifungal drug caspofungin acetate (Cancidas®). However, the low titer of PB results in fermentation and purification costs during caspofungin production, limiting its widespread clinical application. Here, we engineered an efficient PB-producing strain of by systems metabolic engineering strategies, including multi-omics analysis and multilevel metabolic engineering.

Effectiveness of oregano essential oil vapor on shelf life extension of kai lan (Brassica oleracea var. alboglabra).

This study compared the antimicrobial activity of several essential oils (EOs) vapor against food spoilage microbiota and further investigated the potential of EO vapor in extending the shelf life of leafy green vegetables. Oregano EO vapor showed stronger antimicrobial activities than basil and clove EO vapors against common spoilage-causing microorganisms in fresh produce, including Pantoea agglomerans, Pseudomonas cichorii, Pectobacterium carotovorum, Pantoea ananatis, Pseudomonas marginalis, Alterneria bassicicola, and Botrytis cinerea. When oregano EO vapor was applied to leafy greens, phytotoxic effects were observed on butter lettuce and iceberg lettuce but not on kai lan and kale.

Public attitude and media governance of biometric information dissemination in the era of digital intelligence.

Absrtact: In the era of digital intelligence, biometrics plays a critical role in mediating sensitive information dissemination, human-computer interaction, and governance in both virtual and real-world settings, including the evolving metaverse. Based on an empirical analysis of 1,862 participants, the current study investigated factors influencing public perception, acceptance, and risk awareness of biometric technologies. The findings highlight the critical roles of perceived trust (PT) and technical prudence (TP) in driving behavioral intentions (BI), with their positive effects outweighing the significant deterrent impact of perceived risks (PR).

CDK14 regulates the development and repair of lung.

Cyclin-dependent kinases (CDK) 14 regulates cell cycle, tumor expansion by influencing the downstream targets of the canonical Wnt signaling pathway. However, the function of CDK14 during organ development and regeneration has not been investigated in genetically-modified animals. Here, we found that genetic ablation of Cdk14 influenced pulmonary vascular endothelial cells and alveolar epithelial cells during mice embryonic development as well as repair of lung after bleomycin or lipopolysaccharide induced injury.

Polyphenol-modified 3D Nanoassemblies: A novel antibacterial immunoglobulins loading platform for rapid detection of Salmonella typhimurium.

Within the realm of lateral flow assay (LFIA), the conjugation efficiency between signal tracers and antibody constitutes a pivotal determinant for the sensitivity of the detection system. In this study, three-dimensional (3D) complex flower-like MoS self-assembled from 2D MoS, and natural plant polyphenols "Tannic acid" were introduced for surface modification. This composite material exhibits distinct colorimetric signals, excellent monoclonal antibody coupling efficiency, and commendable photothermal properties.