Multicenter evaluation of Neo-Sensitabs, a standardized diffusion method for yeast susceptibility testing.

The agar diffusion method Neo-Sensitabs for sensitivity testing, was evaluated with 33 reference strains by fourteen laboratories. Tablets with 5-fluorocytosine, amphotericin B, nystatin, fluconazole, itraconazole, ketoconazole and tioconazole were used on Shadomy modified medium. These tests classify each strain as susceptible, intermediate or resistant to all tested antifungals by measuring the inhibition zone diameters.

Group 1 beta-lactamases of Aeromonas caviae and their resistance to beta-lactam antibiotics.

The contribution of beta-lactamase production to beta-lactam antibiotic resistance was examined in an Aeromonas caviae mutant strain, selected in vitro by cefotaxime and derived from a wild-type strain isolated in our laboratory from crude sewage. Both strains produced beta-lactamase. The mutant strain (AC7m) produced beta-lactamase constitutively, in contrast to the parental strain (AC7), which was inducible by cefoxitin.

[Carriers of beta hemolytic streptococci from groups A, B, and C among schoolchildren in Las Palmas].

Background: Betahemolytic streptococci, particularly group A, are the most frequently isolated pathogen in the cases of acute pharyngotonsilitis in school-aged children. A study was carry out in school children of Las Palmas de Gran Canaria with the aim of knowing of the state of pharyngeral carriers of betahemolytic streptococci in Lancefield groups A, B and C.

Methods: A total of 1,002 healthy school children (520 boys and 482 girls) with ages ranging from 4 to 15 of aged were included in the study in which a pharyngeal exudate was obtained to detect the presence of betahemolytic streptococci.

[Sensitivity of groups A, B and C beta hemolytic streptococci to antibiotics].

We studied the susceptibility of 330 strains of beta hemolytic streptococci (60 group A, 125 group B, 145 group C) isolated from healthy school children in Las Palmas de Gran Canaria to 17 antibiotics. Only 2% of group A streptococci showed resistance to erythromycin, and 18% to 31% of the beta hemolytic streptococci strains were resistant to trimethoprim-sulfamethoxazole. All of these strains showed susceptibility to betalactam antibiotics, chloramphenicol, clindamycin and vancomycin.

Antibacterial susceptibility patterns of Pseudomonas strains isolated from chronic canine otitis externa.

Development of antibiotic resistance in bacteria is a problem of great concern. It is important to establish the convenience of antimicrobial susceptibility tests in animal infections. The aim of this study was to test the susceptibility to antibiotics of Pseudomonas strains isolated from chronic canine otitis externa.