Publications by authors named "Z Darula"
H2A.Z-nucleosomes are present in both euchromatin and heterochromatin and it has proven difficult to interpret their disparate roles in the context of their stability features. Using an in situ assay of nucleosome stability and DT40 cells expressing engineered forms of the histone variant we show that native H2A.
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- A murine model of colorectal cancer (CRC) was created by injecting CT26 colon carcinoma cells into the spleen of BALB/c mice to study cancer spread to the liver.
- The presence of CRC was confirmed using specific staining techniques, and changes in white blood cell populations were investigated through immunophenotyping and lectin binding assays.
- Proteomic analysis revealed unique protein expressions in CRC-affected tissues, identifying potential biomarkers like Galectin-1 and S100A4 that are linked to poor patient outcomes, which could inform future treatment strategies for human CRC.
Collagen cross-links created by the lysyl oxidase and lysyl hydroxylase families of enzymes are a significant contributing factor to the biomechanical strength and rigidity of tissues, which in turn influence cell signaling and ultimately cell phenotype. In the clinic, the proteolytically liberated N-terminal cross-linked peptide of collagen I (NTX) is used as a biomarker of bone and connective tissue turnover, which is altered in several disease processes. Despite the clinical utility of these collagen breakdown products, the majority of the cross-linked peptide species have not been identified in proteomic datasets.
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- The study investigates changes in the brain's extracellular fluid (ECF) peptidome during epileptic seizures in a rat model using a drug called 4-aminopyridine (4-AP), which induces seizures.
- Researchers utilized in vivo microdialysis combined with electrophysiology to collect and analyze brain fluid samples, identifying 2540 peptides, with 866 linked to proteins that were altered during seizures.
- Key findings reveal that certain neuropeptides and proteins involved in synaptic regulation and astrocyte function significantly changed following seizures, highlighting the potential of ECF analysis for monitoring brain activity and damage.
The rotifer-specific biopolymer, namely Rotimer, is a recently discovered group of the biomolecule family. Rotimer has an active role in the biofilm formation initiated by rotifers (e.g.
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