Near-Native Imaging of Metal Ion-Initiated Cell State Transition.

Metal ions are indispensable to life, as they can serve as essential enzyme cofactors to drive fundamental biochemical reactions, yet paradoxically, excess is highly toxic. Higher-order cells have evolved functionally distinct organelles that separate and coordinate sophisticated biochemical processes to maintain cellular homeostasis upon metal ion stimuli. Here, we uncover the remodeling of subcellular architecture and organellar interactome in yeast initiated by several metal ion stimulations, relying on near-native three-dimensional imaging, cryo-soft X-ray tomography.

Insights into the role of dissolved organic matter derived from paddy soils with different parent materials in the environmental behavior of heavy metal adsorbed by ferrihydrite.

The interaction between dissolved organic matter (DOM) and ferrihydrite (Fh) is a crucial process to control the environmental behavior of heavy metals (HMs) in soil environments, with DOM playing a particularly strong role in HMs fate. Since chemical properties of DOM vary based on different soil parent materials, the underlying impact of DOM-Fh associations on HMs binding remains unclear. This study systematically investigated the interactions between DOM from three soil parent materials (fluvial alluvium: FDOM, sand-shale: SDOM and granite: GDOM) and Fh, and meanwhile understand their effects on the environmental behavior of Cd and Pb under various environmental conditions.

Identification of GBN5 as a molecular biomarker of pan-cancer species by integrated multi-omics analysis.

Introduction: We conducted a panoramic analysis of GBN5 expression and prognosis in 33 cancers, aiming to deepen the systematic understanding of GBN5 in cancer.

Materials And Methods: We employed a multi-omics approach, including transcriptomic, genomic, proteomic, single-cell cytomic, spatial transcriptomic, and genomic data, to explore the prognostic value and potential oncogenic mechanisms of GBN5 across pan-cancers from multiple perspectives.

Results: We found that GBN5 was differentially expressed in multiple tumors and showed early diagnostic value.

Degenerative Disease Diagnosis and Analysis Based on Tissue Specificity of DNA Methylation.

The tissue specificity of DNA methylation refers to the significant differences in DNA methylation patterns in different tissues. This specificity regulates gene expression, thereby supporting the specific functions of each tissue and the maintenance of normal physiological activities. Abnormal tissue-specific patterns of DNA methylation are closely related to age-related diseases.

Metagenomic and enzymatic mechanisms underpinning efficient water treatment of 2-ethylhexyl diphenyl phosphate (EHDPP) by the microbial consortium 8-ZY.

The ubiquitous presence, potential toxicity, and persistence of 2-ethylhexyl diphenyl phosphate (EHDPP) in the environment have raised significant concerns. In this study, we successfully isolate a novel microbial consortium, named 8-ZY, and we demonstrate its remarkable ability to degrade EHDPP using an extremely low concentration of the inoculate. A total of 11 degradation metabolites were identified, including hydrolysis, hydroxylated, methylated, glucuronide-conjugated, and previously unreported byproducts, enabling us to propose new transformation pathways.