In-Cell Mass Spectrometry and Ultraviolet Photodissociation Navigates the Intracellular Protein Heterogeneity.

Directly probing the heterogeneous conformations of intracellular proteins within their native cellular environment remains a significant challenge in mass spectrometry (MS). Here, we establish an in-cell MS and ultraviolet photodissociation (UVPD) strategy that directly ejects proteins from living cells into a mass spectrometer, followed by 193 nm UVPD for structural analysis. Applying this approach to calmodulin (CaM), we reveal that it adopts more extended conformations within living cells compared with purified samples , highlighting the unique influence of intracellular environments on protein folding.

The neutrophil-lymphocyte ratio as a risk factor for all-cause mortality among individuals with resolved HBV infection: evidence from the NHANES 1999-2018.

Background: Inflammation is a critical component in the process of resolved hepatitis B virus (HBV) infection. The neutrophil-to-lymphocyte ratio (NLR) serves as a sensitive indicator of systemic inflammation and immune activation. Our study aimed to investigate the correlation between elevated NLR levels and the risk of all-cause mortality in patients with resolved HBV infection.

The relationship between moral sensitivity and prosocial behavior in college students: the mediating roles of moral disengagement and reciprocity norms.

Introduction: This study examined the relationship between moral sensitivity and prosocial behavior, as well as the mediating role of moral disengagement and reciprocity norms.

Methods: Totally 567 college students completed Dispositional Moral Sensitivity Questionnaire, Moral Disengagement Scale for Chinese Students, Reciprocity Norm Scale, Prosocial Tendencies Measure for Adolescent.

Results: The results showed that, moral disengagement and reciprocity norms played multiple mediating roles between moral sensitivity and prosocial behavior.

From micro to macro, nanotechnology demystifies acute pancreatitis: a new generation of treatment options emerges.

Acute pancreatitis (AP) is a disease characterized by an acute inflammatory response in the pancreas. This is caused by the abnormal activation of pancreatic enzymes by a variety of etiologic factors, which results in a localized inflammatory response. The symptoms of this disease include abdominal pain, nausea and vomiting and fever.

Screening for Type 2 Diabetes Mellitus: A Systematic Review of Recent Economic Evaluations.

Objectives: To examine recent economic evaluations, whether any type 2 diabetes mellitus (T2DM) screening designs may represent better value for money, and to rate their methodological qualities.

Methods: We systematically searched three concepts (economic evaluations (EEs), T2DM, screening) in three databases (Medline, Embase, and EconLit) for EEs published between 2010 and 2023. Two independent reviewers screened for and rated their methodological quality (using CHEC-Extended).