Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples.

PCR and its variants (RT-PCR and qRT-PCR) are valuable and innovative molecular techniques for studying nucleic acids. qPCR has proven to be highly sensitive, efficient, and reproducible, generating reliable results that are easy to analyze. During the COVID-19 pandemic, qPCR became the gold standard technique for detecting the SARS-CoV-2 virus that allowed to confirm the infection event, and those asymptomatic ones, and thus save millions of lives.

Draft genome of the 24Kpn33 and complete sequence of its pCOL-1, a plasmid related to the acquisition in .

We report the draft genome of a clinical multi-resistant (24Kpn33) isolate, whose genome (5.7 Mbp) harbored 17 antibiotic resistance genes, including . Notably, this gene was mobilized within the IncP-6 pCOL-1 plasmid, the first genetic platform related to the acquisition and dissemination of the in .

Worldwide Dissemination of Gene by Novel Mobilization Platforms in : A Systematic Review.

The dissemination of -harboring (KPC-) is considered a serious public health problem. This study provides an overview of the epidemiology of these isolates to try to elucidate novel mobilization platforms that could contribute to their worldwide spread. A systematic review in PubMed and EMBASE was performed to find articles published up to June 2022.

Within patient genetic diversity of bla harboring Klebsiella pneumoniae in a Colombian hospital and identification of a new NTE platform.

Resistance to carbapenems in Klebsiella pneumoniae has been mostly related with the worldwide dissemination of KPC, largely due to the pandemic clones belonging to the complex clonal (CC) 258. To unravel bla post-endemic clinical impact, here we describe clinical characteristics of 68 patients from a high complexity hospital, and the molecular and genetic characteristics of their 139 bla-K. pneumoniae (KPC-Kp) isolates.