Polyoma antigen driven T lymphocytes with antitumor and DTH activity in CBA mice.

T lymphocytes isolated from a bulk culture of splenocytes exposed in vitro to polyoma TAA and IL-2, taken from CBA mice with transplanted polyoma tumors, exhibited both antitumor and DTH activity to polyoma TAA, associated with the Lyt 1+ phenotype, and also showed an increased affinity to TAA. The antitumor efficacy of adoptive immunotherapy, consisting in the i.p.

Delayed hypersensitivity to polyoma and "self" antigens in syngeneic mice.

Using the tail swelling test, delayed-type hypersensitivity (DTH) to tumor and self antigens accompanying the development of syngeneic polyoma tumor in CBA mice was observed. The soluble polyoma cell-surface antigen contained both polyoma and H-2k specificities and induced weak proliferation response of spleen cells in the presence of Il-2 in vitro. Appearance of a measurable tail swelling reaction at the side of the midtail, subcutaneous injection of the eliciting antigen not earlier than at 16th hour, maximum of swelling after 24 hours and the results of histological examination proved that the swelling was caused by a typical DTH to the antigens.