Publications by authors named "Yvonne Radon"
Article Synopsis
- In order to effectively study biological networks, it's crucial to measure multiple biological signals in single living cells due to variations between cells.
- Researchers developed three unique FRET biosensors that can separately track different protein interactions to overcome the challenges of measuring more than two signals simultaneously.
- By applying these biosensors to monitor caspase activity during apoptosis, the study demonstrates how their signals can be used to refine models of biological signaling networks and improve our understanding of how signals propagate within cells.
Cells communicate with their environment via proteins, located at the plasma membrane separating the interior of a cell from its surroundings. The spatial distribution of these proteins in the plasma membrane under different physiological conditions is of importance, since this may influence their signal transmission properties. In this study, the authors compare different methods such as hierarchical clustering, extensible Markov models and the gammics method for analysing such a spatial distribution.
View Article and Find Full Text PDFSignaling from the epidermal growth factor receptor (EGFR) via phosphorylation on its C-terminal tyrosine residues requires self-association, which depends on the diffusional properties of the receptor and its density in the plasma membrane. Dimerization is a key event for EGFR activation, but the role of higher order clustering is unknown. We employed single particle tracking to relate the mobility and aggregation of EGFR to its signaling activity.
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- - The study investigates the role of specific molecular determinants in prion protein (PrP) that influence its misfolding, aggregation, and neurotoxicity, particularly focusing on how these properties impact cellular functions.
- - Using Drosophila S2 and human MCF-7 cells, as well as zebrafish models, the researchers found that certain mutations in PrP affect its location within the cell and its biological activities, indicating a strong correlation between structural features and functionality.
- - Key findings suggest that the N-terminal leader peptide is critical for PrP targeting to cell contact sites, while other domains orchestrate its signaling and distribution, with conservation of these roles observed across both mice and zebrafish species.
The lipid raft proteins reggie-1 and -2 (flotillins) are implicated in membrane protein trafficking but exactly how has been elusive. We find that reggie-1 and -2 associate with the Rab11a, SNX4, and EHD1-decorated tubulovesicular recycling compartment in HeLa cells and that reggie-1 directly interacts with Rab11a and SNX4. Short hairpin RNA-mediated down-regulation of reggie-1 (and -2) in HeLa cells reduces association of Rab11a with tubular structures and impairs recycling of the transferrin-transferrin receptor (TfR) complex to the plasma membrane.
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