Identification of core immune-related genes CTSK, C3, and IFITM1 for diagnosing Helicobacter pylori infection-associated gastric cancer through transcriptomic analysis.

Objectives: To identify diagnostic genes and mechanisms linking Helicobacter pylori (H. pylori) infection to gastric cancer.

Methods: Gene expression profiles from GEO were analyzed using differential expression gene (DEG) analysis, weighted gene co-expression network analysis (WGCNA), and functional enrichment.

Two Cases of -Induced Infective Endocarditis.

is a prevalent gram-positive intracellular zoonotic pathogen that is frequently associated with foodborne illnesses and opportunistic infections. This bacterium is responsible for causing various clinical manifestations, including bacteremia, meningitis, and encephalitis, and is primarily transmitted through contaminated food consumption. This study presents two cases of severe endocarditis in patients with heart valve disease caused by .

Different clinicopathological features between young and older patients with pulmonary adenocarcinoma and ground-glass opacity.

After the recommendation of computed tomography as a routine procedure for lung cancer screening, an increasing number of young adults have been diagnosed with pulmonary ground-glass opacity (GGO). Up to 63% of pulmonary nodules with a GGO component can be malignant. Since young cancer patients have limited exposure to environmental mutagens, they have special characteristics and needs.

Highly sensitive detection of gold and magnetic nanoparticle-mediated sandwich hybridization coupled with ICP-MS.

Foodborne pathogens including () are responsible for over 600 million global incidences of illness annually, posing a significant threat to public health. Inductively coupled plasma mass spectrometry (ICP-MS), coupled with element labeling strategies, has emerged as a promising platform for multivariate and accurate pathogen detection. However, achieving high specificity and sensitivity remains a critical challenge.

Pathogen detection via inductively coupled plasma mass spectrometry analysis with nanoparticles.

Infections caused by viruses and bacteria pose a significant threat to global public health, emphasizing the critical importance of timely and precise detection methods. Inductively coupled plasma mass spectrometry (ICP-MS), a contemporary approach for pathogen detection, offers distinct advantages such as high sensitivity, a wide linear range, and multi-index capabilities. This review elucidates the underexplored application of ICP-MS in conjunction with functional nanoparticles (NPs) for the identification of viruses and bacteria.

[Mechanisms of Intracellular Infection and Reflections Concerning Clinical Practice].

(), for a long time, has generally been considered an extracellular bacterium. However, recent findings have shown that can gain entry into host cells, evade attacks from the host immune system and the killing ability of medication, form stable intracellular ecological niche, and achieve re-release into the extracellular environment, thus causing recurrent infections. intracellular infection causes cellular signaling and metabolic alterations, which may be closely associated with the pathogenesis and progression of tumors, thereby presenting new challenges for clinical eradicative treatment of .

Inhibitory and Injury-Protection Effects of O-Glycan on Gastric Epithelial Cells Infected with Helicobacter pylori.

Helicobacter pylori (H. pylori) is an important pathogen that can cause gastric cancer. Multiple adhesion molecules mediated H.

[Sensitivity Comparison Experiment of Four Testing Methods for ].

Objective: To measure with standard microbiology methods the sensitivity of 4 commonly used testing methods for (Hp) and to conduct a comparative study of the correlations and differences across the 4 methods.

Methods: With the Hp standard strain (SS1) as the reference, colony forming units (CFU) as the units of quantitative analysis for detection performance, and gradient dilution of SS1 suspension as the simulation sample, we measured the sensitivity of 4 Hp testing methods, including bacterial culture, rapid urease test, antigen test, and quantitative fluorescent PCR. CFU values at different concentrations corresponding to the 4 commonly used Hp testing methods were documented and the correlations and differences were analyzed accordingly.

Role of lipid rafts in persistent infection: a narrative review.

Background And Objective: The distribution of components in the cell membrane is not uniform, but is organized into specific functional microdomains, known as "lipid rafts". These lipid rafts consist of cholesterol, sphingolipids, and various proteins. Studies have shown that lipid rafts contain multiple proteins that are closely related to signal transduction and immune response.

Study on the preparation and function of regulatory T cells from human peripheral blood.

Background: Regulatory T cells (Tregs) are an important cell subgroup of CD4 T cells. Treg cells are critically involved in inducing immune tolerance, maintaining immune environment homeostasis, and preventing the occurrence of autoimmune diseases. Under normal conditions, the number of Tregs in the body is very small.

[Preparation and Release Properties of Recombinant Protein PLGA Microspheres and PLGA-Chitosan Microspheres].

Objective: To preparethe poly lactic-co-glycolic acid (PLGA) microspheres and PLGA-chitosan microspheres containing recombinant protein, namely the BIB protein, and to explore their optimal preparation parameters and release performance in gastric and intestinal fluids.

Methods: Double emulsions (water-in-oil-in-water, or W1/O/W2) solvent evaporation method was used to prepare the BIB-PLGA microspheres and the BIB-PLGA-chitosan microspheres. Univariate analysis was done to study the impact of the water-to-oil ratio (W1/O), PLGA mass fraction and PVA concentration on the morphology, particle size, polydispersity index (PDI), encapsulation efficiency (EE), and drug loading (DL) so as to identify the optimal parameters.

Anti-bacterial and anti-viral effects of Fengreqing oral liquid () in vitro and in vivo.

Objective: To investigate the anti-bacterial and anti-viral effects of Fengreqing oral liquid (, FRQ) in vitro and in vivo.

Methods: The minimum inhibitory concentrations of Fengreqing Oral Liquid against six gram-positive bacteria (Staphylococcus aureus, Streptococcus mutans, Peptostreptococcus anaerobius, Hemolytic streptococcus, Streptococcus pneumoniae, Klebsiella pneumoniae), seven gram-negative bacteria (Escherichia coli, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Haemophilus influenzae, Helicobacter pylori, Pseudomonas aeruginosa, Gardnerella vaginalis) and Candida albicans were detected by the paper disc diffusion method. The inhibition rate of A/PuertoRico/8/34(H1N1) (PR8) influenza virus in different concentrations of Fengreqing oral solution was detected by chicken embryo method.