Characterisation of aroma compounds, sensory characteristics, and bioactive components of a new type of huangjiu fermented with Chinese wild rice (Zizania latifolia).

Chinese wild rice (CWR) is a nutritious and healthy whole grain, worth developing. To develop and use its value, a new type of huangjiu was brewed with CWR, and the flavour characteristics, sensory quality, functional and bioactive components were evaluated. CWR (67 flavour substances) and glutinous rice (GR)-CWR huangjiu (62 flavour substances) had a better flavour than GR huangjiu (54 flavour substances), and the overall style of GR-CWR huangjiu was more skewed towards GR.

Enzymatic properties and inhibition tolerance analysis of key enzymes in β-phenylethanol anabolic pathway of HJ.

is known for its unique aroma, primarily attributed to its high concentration of β-phenylethanol (ranging from 40 to 130 mg/L). Phenylalanine aminotransferase Aro9p and phenylpyruvate decarboxylase Aro10p are key enzymes in the β-phenylethanol synthetic pathway of HJ. This study examined the enzymatic properties of these two enzymes derived from HJ and S288C.

Saccharomyces cerevisiae strains with low-yield higher alcohols and high-yield acetate esters improve the quality, drinking comfort and safety of huangjiu.

Higher alcohols (HAs) and acetate esters (AEs) produced by yeasts are two important volatile flavor substances in fermented alcoholic beverages (FABs). To improve the FABs overall quality, lab-scale huangjiu brewing and systematic evaluation were performed using 171 Saccharomyces cerevisiae strains. Finally, two S.

Combined effects of fermentation temperature and Saccharomyces cerevisiae strains on free amino acids, flavor substances, and undesirable secondary metabolites in huangjiu fermentation.

Fermentation temperature (FT) is one of the most critical factors, which can be used to control the growth conditions of S. cerevisiae to obtain excellent final products in winemaking. In this study, we analyzed the responses of six S.

Replicon-Based Typing of IncI-Complex Plasmids, and Comparative Genomics Analysis of IncIγ/K1 Plasmids.

IncI-complex plasmids can be divided into seven subgroups IncI1, IncI2, IncIγ, IncB/O, IncK1, IncK2, and IncZ. In this study, a replicon-based scheme was proposed for typing IncI-complex plasmids into four separately clustering subgroups IncI2, IncI1/B/O, IncIγ/K1 and IncK2/Z, the last three of which were combined from IncI1 and IncB/O, IncIγ and IncK1, and IncK2 and IncZ, respectively. Four IncIγ/K1 plasmids p205880-NR2, p14E509-CTXM, p11011-CTXM and p61806-CTXM were fully sequenced and compared with IncIγ/K1 reference pCT, IncI2 reference R721, IncI1/B/O reference R64 and IncK2/Z reference pO26-CRL-125.

Comparative analysis of - and -carrying IncFII-family pKPC-LK30/pHN7A8 hybrid plasmids from CG258 strains disseminated among multiple Chinese hospitals.

Background: We recently reported the complete sequence of a KPC-2- and -carrying IncFII-family plasmid p675920-1 with the pKPC-LK30/pHN7A8 hybrid structure. Comparative genomics of additional sequenced plasmids with similar hybrid structures and their prevalence in -carrying strains from China were investigated in this follow-up study.

Methods: A total of 51 -carrying strains were isolated from 2012 to 2016 from five Chinese hospitals and genotyped by multilocus sequence typing.

Comparative genomics of type 1 IncC plasmids from China.

Aim: This study dealt with genomic characterization of type 1 IncC resistance plasmids, capable of spreading across taxonomic borders, from China.

Materials & Methods: p112298-tetA was sequenced and compared with type 1 IncC reference plasmid pR148 and two available sequenced type 1 IncC plasmids pHS36-NDM and pVAS3-1 from China.

Results: These plasmids contained one or more exogenous resistance islands, which included the ARI-A islands, the ARI-B islands, the ISEcp1-bla units and the bla region and were inserted at various sites in the IncC backbone and thus represented three distinct lineages.

p1220-CTXM, a pKP048-related IncFII plasmid carrying bla and qnrB4.

Aim: This study aimed to characterize plasmid-mediated antimicrobial resistance in clinical Klebsiella pneumoniae 1220 carrying bla and qnrB4.

Materials & Methods: Plasmid p1220-CTXM was transformed from the 1220 isolate into Escherichia coli through conjugal transfer and then fully sequenced. Antimicrobial susceptibility was determined by VITEK.