Splicing Factor PTBP1 Silencing Induces Apoptosis of Human Cervical Cancer Cells via PI3K/AKT Pathway and Autophagy.

Background: Cervical cancer is the most common gynecological malignancy in the world and seriously threatens to women's lives and health. Polypyrimidine tract binding protein 1 (PTBP1), as an important splicing factor, has been identified as a proto-oncogene in several cancers, but its role and mechanism in cervical cancer remain poorly understood. Thus, our aim is to explore the impact of PTBP1 on proliferation, migration, apoptosis of cervical cancer cells, and its underlying mechanisms.

Reducing postoperative hypothermia in infants: Quality improvement in China.

Background: Unintended postoperative hypothermia in infants is associated with increased mortality and morbidity. We noted consistent hypothermia postoperatively in more than 60% of our neonatal intensive care (NICU) babies. Therefore, we set out to determine whether a targeted quality improvement (QI) project could decrease postoperative hypothermia rates in infants.

[Effect of strontium in drinking water on blood pressure and inflammatory function in hypertension mice].

Objective: To explore the effect of strontium in drinking water on blood pressure in hypertensive mice and its possible mechanism.

Methods: Establishment of mouse model of high blood pressure by drinking 2 mg/mL N'nitro-L-arginine methyl eater hydrochloride(L-NAME) for 4 weeks. One hundred ICR mice were randomly divided into normal control group(n=20) and model group(n=80) according to systolic blood pressure.

From electronic structure to model application for alkyl cyclohexane combustion chemistry: H-atom abstraction reactions by HȮ radical.

Chemical kinetic studies of hydrogen atom abstraction reactions by hydroperoxyl (HȮ) radical from six alkyl cyclohexanes of methyl cyclohexane (MCH), ethyl cyclohexane (ECH), -propyl cyclohexane (PCH), iso-propyl cyclohexane (iPCH), -butyl cyclohexane (BCH), and iso-butyl cyclohexane (iBCH) are carried out systematically through high-level calculations. Geometry optimizations and frequency calculations for all species involved in the reactions are performed at the M06-2X/6-311++G(d,p) level of theory. Electronic single-point energy calculations are calculated at the UCCSD(T)-F12a/cc-pVDZ-F12 level of theory, with zero-point energy corrections.

Liver Immune Profiling Reveals Pathogenesis and Therapeutics for Biliary Atresia.

Biliary atresia (BA) is a severe cholangiopathy that leads to liver failure in infants, but its pathogenesis remains to be fully characterized. By single-cell RNA profiling, we observed macrophage hypo-inflammation, Kupffer cell scavenger function defects, cytotoxic T cell expansion, and deficiency of CX3CR1effector T and natural killer (NK) cells in infants with BA. More importantly, we discovered that hepatic B cell lymphopoiesis did not cease after birth and that tolerance defects contributed to immunoglobulin G (IgG)-autoantibody accumulation in BA.

Metabolite signatures of diverse Camellia sinensis tea populations.

The tea plant (Camellia sinensis) presents an excellent system to study evolution and diversification of the numerous classes, types and variable contents of specialized metabolites. Here, we investigate the relationship among C. sinensis phylogenetic groups and specialized metabolites using transcriptomic and metabolomic data on the fresh leaves collected from 136 representative tea accessions in China.

Irrelevance of USF2 rs916145 polymorphism with the risk of biliary atresia susceptibility in Southern Chinese children.

Backgrounds: Biliary atresia (BA) is a very rare neonatal disease, however, it has been the most common cause of obstructive jaundice in infancy. The complex pathogenesis of BA is not entirely clear and a lot of possible pathogenic mechanisms have been proposed to explain the etiology of BA, including genetic, inflammatory, environmental and developmental abnormalities. As a transcription factor, USF2 gene rs916145 polymorphism has been shown to be related to the risk of BA.

Defensive Responses of Tea Plants () Against Tea Green Leafhopper Attack: A Multi-Omics Study.

Tea green leafhopper [ () Matsuda] is one of the most devastating pests of tea plants (), greatly impacting tea yield and quality. A thorough understanding of the interactions between the tea green leafhopper and the tea plant would facilitate a better pest management. To gain more insights into the molecular and biochemical mechanisms behind their interactions, a combined analysis of the global transcriptome and metabolome reconfiguration of the tea plant challenged with tea green leafhoppers was performed for the first time, complemented with phytohormone analysis.

Associations Between Sleep Quality and Migraine Frequency: A Cross-Sectional Case-Control Study.

Migraine has been associated with sleep disturbances. Relationship between sleep quality and migraine frequency is yet to be determined. The present study aimed to investigate sleep disturbances among low-frequency, moderate-frequency, high-frequency, and chronic migraineurs, with and without auras, with well-controlled confounding variables.

Tunable diode laser absorption spectroscopy-based tomography system for on-line monitoring of two-dimensional distributions of temperature and H2O mole fraction.

To monitor two-dimensional (2D) distributions of temperature and H2O mole fraction, an on-line tomography system based on tunable diode laser absorption spectroscopy (TDLAS) was developed. To the best of the authors' knowledge, this is the first report on a multi-view TDLAS-based system for simultaneous tomographic visualization of temperature and H2O mole fraction in real time. The system consists of two distributed feedback (DFB) laser diodes, a tomographic sensor, electronic circuits, and a computer.

Development of a fan-beam TDLAS-based tomographic sensor for rapid imaging of temperature and gas concentration.

This work aims to develop a fan-beam tomographic sensor using tunable diode lasers that can simultaneously image temperature and gas concentration with both high spatial and temporal resolutions. The sensor features three key advantages. First, the sensor bases on a stationary fan-beam arrangement, by which a high spatial resolution is guaranteed because the distance between two neighboring detectors in a view is approximately reduced to the size of a photodiode.

Epidemiology of hospitalized traumatic pelvic fractures and their combined injuries in Taiwan: 2000-2011 National Health Insurance data surveillance.

Background: From the viewpoint of prehospital emergency medicine, a greater proportion of pelvic fractures not of a life-threatening status but combined with other injuries need more comprehensive recognition.

Methods: A 12-year nationwide health database of inpatients was reviewed. All cases diagnosed as pelvic fractures were enrolled.

Diagnostic validity of human papillomavirus E6/E7 mRNA test in cervical cytological samples.

Human papillomavirus (HPV) DNA tests tend to show high sensitivity, but poor specificity in detecting high-grade cervical lesions. This study aimed to explore the clinical performance of QuantiVirus(®) HPV E6/E7 mRNA in identifying ≥Grade 2 cervical intraepithelial neoplasia. Thin-prep(®) liquid based cytology test (LBC) samples were collected from October 2009 to October 2011 from women who underwent outpatient hospital-based gynecological screening.

[Biodegradation mechanism of DDT and chlorpyrifos using molecular simulation].

In order to explore the microscopic degradation mechanism of organic pesticides degrading enzymes, we used molecular docking method to investigate the binding modes of DDT to laccase and chlorpyrifos to organophosphorus hydrolase, and obtained the corresponding complex structures. According to the principle of minimum scoring, the results showed that the MolDock scores were -103.134 and -111.

Compound microsatellites in complete Escherichia coli genomes.

Compound microsatellites consisting of two or more repeats in close proximity have been found in eukaryotic genomes. So far such compound microsatellites have not been investigated in any prokaryotic genomes. We have therefore examined compound microsatellites in 22 complete genomes of Escherichia coli, which is one of the ideal model organisms to analyze the nature and evolution of prokaryotic compound microsatellites.

[Retroperitoneal lymph node metastasis in epithelial cancer of the ovary: a report of 58 cases].

Objective: To analyze the retroperitoneal lymph node metastasis in epithelial cancer of the ovary and offer scientific indications for lymph node radical dissection.

Methods: Fifty-eight patients with ovarian cancer treated from January 1990 to December 2000 were retrospectively reviewed. Single-factor and multifactor analysis with Logistic regression model were performed by SPSS 10.

Morphology of the tibial organs of acrididae: Comparison of subgenual and distal organs in fore-, mid-, and hindlegs of Schistocerca gregaria (Acrididae, Catantopinae) and Locusta migratoria (Acrididae, Oedipodinae).

The structure of the complex tibial organs in the fore-, mid-, and hindlegs of two grasshopper species, Schistocerca gregaria (Acrididae, Catantopinae) and Locusta migratoria (Acrididae, Oedipodinae), is described. In each leg the tibial organs consist of two scolopale organs: the subgenual organ and the distal organ. Both organs are located in the hemolymph channel.

Complex tibial organs in fore-, mid-, and hindlegs of the bushcricket Gampsocleis gratiosa (Tettigoniidae): Comparison of morphology of the organs.

The structure of the complex tibial organs in the fore-, mid-, and hindlegs of the East Asian bushcricket Gampsocleis gratiosa (Tettigoniidae, Decticinae) is described comparatively. In each leg the tibial organs consist of three scolopale organs: the subgenual organ, the intermediate organ, and the crista acoustica. Only in the forelegs are the tibial organs differentiated as tympanal organs, and sound transmitting structures (acoustic trachea, tympana, and tympanal covers) are present.