The metabolomics provides insights into the Pacific abalone () response to low temperature stress.

The low temperatures in winter, particularly the cold spells in recent years, have posed significant threats to China's abalone aquaculture industry. The low temperature tolerance of cultured abalone has drawn plenty of attention, but the metabolic response of abalone to low-temperature stress remains unclear. In this study, we investigated the metabolomic analysis of Pacific abalone () during low-temperature stress.

Shellfish CO excretion is modulated by seawater carbonate chemistry but largely independent of pCO.

Four species of shellfish, blue mussel (Mytilus galloprovincialis), Pacific abalone (Haliotis discus hannai), zhikong scallops (Chlamys farreri), and Pacific oyster (Crassostrea gigas), were exposed to decoupled carbonate system variables to investigate the impacts of different seawater carbonate parameters on the CO excretion process of mariculture shellfish. Six experimental groups with two levels of seawater pH (pH 8.1 and pH 7.

Assessment of benthic ecological status and heavy metal contamination in an estuarine intertidal mudflat in the Northern Bohai Sea.

Evaluating the ecological quality and pollution status of coastal mudflats is crucial for environmental protection and management, particularly when these areas serve as major shellfish production hotspots. In this study, we assessed the benthic ecological quality and heavy metals pollution in Geligang, located in the Northern Bohai Sea using the macrobenthos diversity index and the heavy metal pollution index. The Shannon-Wiener index (H'), AZTI marine biotic index (AMBI), multivariate AMBI (M-AMBI) showed that the benthic ecological quality in Geligang is either good or high.

A Comparative Study on Effects of Three Butyric Acid-Producing Additives on the Growth Performance, Non-specific Immunity, and Intestinal Microbiota of the Sea Cucumber .

The providers of butyric acid, (CB), sodium butyrate (SB), and tributyrin (TB), have been extensively studied as aquafeed additives in recent years. However, no comparative study has been reported on the probiotic effects of CB, SB, and TB as feed additives on sea cucumber (). A 63-day feeding trial was performed to assess the effects of dietary live cells of (CB group, the basal diet supplemented with 1% CB), sodium butyrate (SB group, the basal diet supplemented with 1% SB), and tributyrin (TB group, the basal diet supplemented with 1% TB) on the growth, non-specific immunity, and intestinal microbiota of with a basal diet group as the control.

Modification of osimertinib to discover new potent EGFR-TK inhibitors.

The EGFR mutation is a dominant mechanism of acquired resistance after the treatment of non-small cell lung cancer (NSCLC) with osimertinib in clinic. To date, there is no inhibitor approved to overcome the resistance caused by osimertinib. In this study, a series of compounds with phenylamino-pyrimidine scaffold deriving from osimertinib were designed, synthesized and evaluated as fourth-generation EGFR-TK inhibitors.

The effect of total alkalinity on growth performance and calcification in juvenile Pacific abalone Haliotis discus hannai.

A 45-day trial was conducted to study the effect of seawater total alkalinity (TA) level up- and downregulation on the growth performance and calcification of Haliotis discus hannai Ino, while seawater pH was maintained at pH = 8.1. Although seawater was not acidified, the results showed that TA downregulation caused a significant reduction (P < 0.

The Application of the Generalized Additive Model to Represent Macrobenthos near Xiaoqing Estuary, Laizhou Bay.

Macrobenthos is widely used as an indicator of ecological health in marine monitoring and assessment. The present study aimed to characterize the interrelationships between the distribution of the macrobenthos community and environmental factors near Xiaoqing Estuary, Laizhou Bay. Responses of species richness to environmental factors were studied using the generalized additive model (GAM) and the Margalef diversity index () as indicators of species diversity instead of individual indicator species.

A food-web model as a tool for the ecosystem-level management of bivalves in an Atlantic coastal lagoon.

The Ria de Aveiro is an important coastal lagoon for wildlife in Portugal, where the production of bivalves reaches approximately 2700 tons annually. However, the illegal overfishing of bivalves is frequent in this lagoon, which causes critical changes in the ecosystem. In this study, using a developed food-web model (Ecopath model), the ecological carrying capacity (ECC) and maximum sustained yield (MSY) of the Manila clam, Ruditapes philippinarum were estimated, and the effects of further increases in clam biomass on other species were investigated.

Design, synthesis and evaluation of new pyrimidine derivatives as EGFR tyrosine kinase inhibitors.

The clinical use of epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) in the treatment of non-small cell lung cancer was limited by the drug resistance caused by EGFR mutation. Therefore, in order to overcome the drug resistance, we designed and synthesized a series of 2-aminopyrimidine derivatives as EGFR-TKIs. Among these compounds, compounds A5 and A13 showed significant anti-proliferative activity against the KC-0116 (EGFR) cell line with high selectivity.

CO-Induced Ocean Acidification Alters the Burrowing Behavior of Manila Clam by Reversing GABA Receptor Function.

Biological burrowing behavior is an important driver shaping ecosystems that is being threatened by CO-induced ocean acidification; however, the effects of ocean acidification on burrowing behavior and its neurological mechanism remain unclear. This study showed that elevated CO significantly affected the burrowing behaviors of the Manila clam , such as increased foot contraction, burrowing time, and intrabottom movement and decreased burrowing depth. Delving deeper into the mechanism, exposure to elevated CO significantly decreased extracellular pH and increased [HCO].

Identification Invasion-Related Long Non-Coding RNAs in Lung Adenocarcinoma and Analysis of Competitive Endogenous RNA Regulatory Networks.

Background: Cell invasion plays a vital role in cancer development and progression. Aberrant expression of long non-coding RNAs (lncRNAs) is also critical in carcinogenesis. However, the prognostic value of invasion-related lncRNAs in lung adenocarcinoma (LUAD) remains unknown.

Haemolymph pH of two important mollusc species is susceptible to seawater buffering capacity instead of pH or pCO.

The acid-base status and balance of molluscs are considered to be susceptible to environmental changes, especially in the context of ocean acidification (OA). Here, we studied the effects of manipulated seawater carbonate chemistry on the acid-base status of scallop Chlamys farreri and abalone Haliotis discus hannai. The haemolymph pH of the tested individuals showed a fast response to acidified seawater incubation, and the pH level was restored to a normal value within 1 h of recovery in control seawater.

A unified model for large-scale thickness measurement of lubricating film based on ultrasonic lag phase slope.

A unified model based on the ultrasonic lag phase slope is developed for measuring the lubricating film thickness at a large scale. The ultrasonic lag phase of adjacent waves instead of the phase of overlapped waves is calculated as a function of the ultrasonic frequency and film thickness. The slope of the ultrasonic lag phase is determined correspondingly, which is linearly proportional to the lubricating film thickness.

Seawater carbonate parameters function differently in affecting embryonic development and calcification in Pacific abalone (Haliotis discus hannai).

pH or pCO are usually taken to study the impact of ocean acidification on molluscs. Here we studied the different impact of seawater carbonate parameters on embryonic development and calcification of the Pacific abalone (Haliotis discus hannai). Early embryonic development was susceptible to elevated pCO level.

Influences of Substrate Grain Size on the Burrowing Behavior of Juvenile .

The substrate is the key environmental factor that affects the growth, survival, population and distribution of dwelling mollusks in mudflat settings. To clarify the effect of the substrate grain size on soft substrate preference, burrowing ability and behavior during the selection process of juvenile Meretrix meretrix, four different grain size substrates (coarse sand, medium sand, fine sand, and natural substrate) were set up for comparison. The results indicated that: (1) the burrowing ability of juvenile specimens in fine sand was the strongest; (2) the degree (from high to low) of the juvenile’s preference for the four substrates was in the order of fine sand > natural substrate > medium sand > coarse sand; and (3) the selection process of the substrate by the juveniles could be divided into four stages: preparation, selection, burrowing and end stages.

Discovery of Potent PROTACs Targeting EGFR Mutants through the Optimization of Covalent EGFR Ligands.

Drug resistance caused by epidermal growth factor receptor (EGFR) mutation has largely limited the clinical use of EGFR tyrosine kinase inhibitors (EGFR-TKIs) for the treatment of non-small-cell lung cancer (NSCLC). Herein, to overcome the intractable problem of drug resistance, proteolysis targeting chimeras (PROTACs) targeting EGFR mutants were developed by optimizing covalent EGFR ligands. Covalent or reversible covalent pyrimidine- or purine-containing PROTACs were designed, synthesized, and evaluated.

The anti-neoplastic activities of aloperine in HeLa cervical cancer cells are associated with inhibition of the IL-6-JAK1-STAT3 feedback loop.

Cervical cancer (CC) is recognized as the most common neoplasm in the female reproductive system worldwide. The lack of chemotherapeutic agents with outstanding effectiveness and safety severely compromises the anti-cipated prognosis of patients. Aloperine (ALO) is a natural quinolizidine alkaloid with marked anti-cancer effects on multiple malignancies as well as favorable activity in relieving inflammation, allergies and infection.

A minimum energy optimization approach for simulations of the droplet wetting modes using the cellular Potts model.

Wetting modes of a droplet on a periodical grooved surface were simulated by using the Cellular Potts Model (CPM). An optimization approach based on the Synthesis Minimum Energy (SME), which is defined as the lowest energy of the simulation system, was proposed for determining the droplet wetting modes. The influence of the fluctuation parameter () was discussed.

Effect of large-scale kelp and bivalve farming on seawater carbonate system variations in the semi-enclosed Sanggou Bay.

Although cultured algae and shellfish can be the dominant species in some localized coastal waters, research on the effect of large-scale mariculture on the carbonate system variations in these local waters is still lacking. We conducted five cruises from May to September and studied spatiotemporal variations in the seawater carbonate system in the semi-closed Sanggou Bay, which is famous for its large-scale mariculture. Our results showed that both kelp and bivalve farming induced significant spatiotemporal variations in the carbonate system within the bay.

Polychlorinated biphenyls and organochlorine pesticides in surface sediments from the sand flats of Shuangtaizi Estuary, China: levels, distribution, and possible sources.

Levels of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) in sediments from the sandy flat system of Shuangtaizi Estuary, the highest-latitude estuary in China, were investigated to identify their possible sources and potential ecological risk. The concentrations of 28 PCBs and 18 OCPs ranged from 1.83 to 36.

Spatial distribution and sources of heavy metals and petroleum hydrocarbon in the sand flats of Shuangtaizi Estuary, Bohai Sea of China.

The concentrations of heavy metals and petroleum hydrocarbons (PHCs) in surface sediments were investigated in the sand flats of Shuangtaizi Estuary, Bohai Sea of China in May, 2013. Ecological risk assessment indicated that most heavy metals cause low ecological risk to the estuarine environment, with the exception of Cd and Hg (considerable and moderate risk, respectively). Principal component analysis in combination with correlation analysis among heavy metals, PHCs and geological factors (e.

Identification and expression analysis on bactericidal permeability-increasing protein (BPI)/lipopolysaccharide-binding protein (LBP) of ark shell, Scapharca broughtonii.

Bactericidal permeability-increasing protein (BPI) and lipopolysaccharide-binding protein (LBP) are the numbers of the lipid transfer protein/lipopolysaccharide-binding protein family and play crucial roles in the innate immune response to Gram-negative bacteria. A novel Sb-BPI/LBP1 from ark shell Scapharca broughtonii was isolated by expressed sequence tag (EST) and RACE techniques. The Sb-BPI/LBP1 cDNA encoded a polypeptide of 484 amino acids with a putative signal peptide of 21 amino acid residues and a mature protein of 463 amino acids.

The impact of the herbicide atrazine on growth and photosynthesis of seagrass, Zostera marina (L.), seedlings.

The impact of the widely used herbicide atrazine on seedling growth and photosynthesis of eelgrass was determined. The long-term impact of the herbicide atrazine (1, 10 and 100 μg/L) on growth of eelgrass Zostera marina (L.) seedlings, maintained in outdoor aquaria, was monitored over 4 weeks.