A Highly Sensitive and Group-Specific Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of AFB in Agriculture and Aquiculture Products.

Aflatoxins (AFs) including AFB, AFB, AFG and AFG are widely found in agriculture products, and AFB is considered one of the most toxic and harmful mycotoxins. Herein, a highly sensitive (at the pg mL level) and group-specific enzyme-linked immunosorbent assay (ELISA) for the detection of AFB in agricultural and aquiculture products was developed. The AFB derivative containing a carboxylic group was synthesized and covalently linked to bovine serum albumin (BSA).

Electrochemiluminescence immunosensor based on tin dioxide quantum dots and palladium-modified graphene oxide for the detection of zearalenone.

Developing low-cost and efficient methods to enhance the electrochemiluminescence (ECL) intensity of luminophores is highly desirable and challenging. Herein, we developed an efficient ECL system based on palladium-modified graphene oxide as a substrate and tin dioxide quantum dot-modified spike-like gold-silver alloy as an immunoprobe. Specifically, palladium-modified graphene oxide was rationally selected as the sensor substrate for the attachment of zearalenone antigens while facilitating the amplification of the ECL signal through enhanced electron transfer efficiency.

A sensitive monoclonal antibody-based ELISA integrated with immunoaffinity column extraction for the detection of zearalenone in food and feed samples.

Zearalenone (ZEN) is one of the most toxic mycotoxins widely found in agricultural products. In this study, a sensitive enzyme-linked immunosorbent assay (ELISA) integrated with immunoaffinity column extraction for the detection of ZEN in food and feed samples was developed. A ZEN derivative containing a carboxylic group was first synthesized and then linked to bovine serum albumin (BSA).

Nomogram predicts risk of perineural invasion based on serum biomarkers for pancreatic cancer.

Background: Pancreatic cancer is a fatal tumor, and the status of perineural invasion (PNI) of pancreatic cancer was positively related to poor prognosis including overall survival and recurrence-free survival. This study aims to develop and validate a predictive model based on serum biomarkers to accurately predict the perineural invasion.

Materials And Methods: The patients from No.

ncRNA-mediated upregulation of is associated with poor prognosis and immune infiltration in pancreatic cancer.

Introduction: Malignant pancreatic cancer has poor long-term survival. Increasing evidence shows that (family with sequence similarity 83 member A) plays a vital role in tumorigenesis and malignant progression in some human cancer types. The present study explored the potential mechanism of in improving the prognosis of pancreatic cancer patients.