Development of a benzene vapour sensor utilizing chemiluminescence on Y2O3.

Chemiluminescence (CL) was observed when benzene vapour passed through the surface of Y(2)O(3), TiO(2), Y(2)O(3)-V(2)O(5), TiO(2)-Y(2)O(3), Y(2)O(3)-Cr(2)O(3), Y(2)O(3)-Al(2)O(3) and TiO(2)-Al(2)O(3), with air as the carrier gas. The strongest CL intensity was found with Y(2)O(3) as the catalyst. A novel benzene sensor based on this kind of CL was developed.

[Construction of hPARP1-deficient cell strain by RNA interference].

Objective: To construct hPARP1-deficient cell strain which can be used in studying the functions and action mechanisms of hPARP1 gene.

Methods: 16-HBEs were transfected with two kinds of eukaryotic expression plasmids of hPARP1 gene short hairpin RNA to construct hPARP1-deficient cells (named as "16-HBEP1 and 16-HBEP2") and a negative control plasmids (named as 16-HBEN). The protein expression levels of hPARP1 gene in 16-HBE, 16-HBEP1 16-HBEP2 and 16-HBEN were detected by the western blotting to estimate the effects of RNA interference.