Remodeling of bacterial nitrilase active pocket improves the capability to degrade glucosinolate-derived nitriles.

Nitriles exhibit acute cytotoxicity to human and animal cells. Nitrilase is a green biocatalyst that can directly convert nitrile into nontoxic carboxylic acids and ammonia. However, the nitrilases capable of degrading 3-butenenitrile and 4-pentenenitrile derived from glucosinolate present in rapeseed meals are still limited.

Nitrilase GiNIT from Efficiently Degrades Nitriles Derived from Rapeseed Meal Glucosinolate.

Rapeseed meal is severely restricted in its utilization as unconventional animal feed due to anti-nutritive compounds, such as glucosinolate, that are degraded to toxic nitriles such as 3-butenenitrile and 4-pentenenitrile in animals. Few studies on nitrilases that can degrade glucosinolate-derived nitriles have been reported thus far. In the present study, a nitrilase gene from was over-expressed in and the purified recombinant nitrilase rGiNIT showed specific activities of 134.

Increasing Nitrogen Losses Due to Changing Food Consumption Patterns in Bayannur City, China.

Increasing urbanization and affluence have led to changes in food consumption patterns. The application of nitrogen (N) fertilizers ensures food security but also leads to environmental pollution due to N losses, through processes such as acidification, eutrophication, and greenhouse gas emissions. To clarify whether changes in food consumption patterns could increase N losses and to explore sustainable food system pathways, this study integrated the Chinese Food System Dashboard and the Nutrient Flows in Food Chains, Environment and Resources Use model to quantify and compare the link between food consumption and N losses in different agricultural regions using a case study of Bayannur City in the Yellow River Basin from 2000 to 2016.

Life cycle cost analysis of solar energy via environmental externality monetization.

Evaluating the embodied environmental impact of solar photovoltaic (PV) technology has been an important topic in addressing the sustainable development of renewable energy. While monetization of environmental externality is a remaining issue, which should be carried out in order to allow for an easy-to-understand comparison between direct economic and external cost. In this study, the environmental impact of solar PV power is monetized through conversion factors between midpoint and endpoint categories of life cycle analysis and the monetization weighting factor.

Network Traffic Prediction Incorporating Prior Knowledge for an Intelligent Network.

Network traffic prediction is an important tool for the management and control of IoT, and timely and accurate traffic prediction models play a crucial role in improving the IoT service quality. The degree of burstiness in intelligent network traffic is high, which creates problems for prediction. To address the problem faced by traditional statistical models, which cannot effectively extract traffic features when dealing with inadequate sample data, in addition to the poor interpretability of deep models, this paper proposes a prediction model (fusion prior knowledge network) that incorporates prior knowledge into the neural network training process.

Role and mechanism of the Dectin-1-mediated Syk/NF-κB signaling pathway in infection.

Dendritic cell-associated C-type lectin-1 (Dectin-1), a C-type lectin receptor, serves a critical role in host antifungal immunity. However, the molecular mechanism and function of Dectin-1-mediated signaling in response to infection by the pathogenic fungus remains unclear. To understand the role of Dectin-1 signaling against infection, the phosphorylation of spleen tyrosine kinase (Syk), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, α (IκBα) and NF-κB were analyzed using western blotting, and the secretion of cytokines was detected using ELISA.

A bifunctional fluorescent probe for simultaneous detection of GSH and HS (n > 1) from different channels with long-wavelength emission.

In this work, we presented a long-wavelength emission fluorescent probe DCM-Cou-SePh that can discriminatively detect glutathione (GSH) and hydrogen polysulfides (HS, n > 1) from green and red emission channels, respectively. With the addition of GSH, probe DCM-Cou-SePh displayed green fluorescence emission (λ = 430/530 nm). In the presence of HS, the probe exhibited a significant fluorescence enhancement in red channel (λ = 560/680 nm).

Anti-ST2 Nanoparticle Alleviates Lung Inflammation by Targeting ILC2s-CD4T Response.

Background: Asthma has been regarded as an inflammatory disease, and group 2 innate lymphoid cells (ILC2s) are implicated in asthma pathogenesis. However, no strategy is available to block ILC2s function. Efficiency is also limited due to the use of systemic or subcutaneous routes of administration.

High-Voltage Resistant Ionic Liquids for Lithium-Ion Batteries.

With the growing demand for high energy and high power density rechargeable lithium-ion batteries, increasing research is focused on improving the output voltage of these batteries. Herein, a series of pyrrolidinium and piperidinium cations with various N-substituents (including cyanomethyl, benzyl, butyl, hexyl, and octyl groups) were synthesized and investigated with respect to their electrochemical stability under high voltages. The influence of substitutions at the N-position of pyrrolidinium and piperidinium cations on their high-voltage resistance was studied by both theoretical and experimental approaches.

Time preferences are reliable across time-horizons and verbal versus experiential tasks.

Unlabelled: Individual differences in delay-discounting correlate with important real world outcomes, for example education, income, drug use, and criminality. As such, delay-discounting has been extensively studied by economists, psychologists and neuroscientists to reveal its behavioral and biological mechanisms in both human and non-human animal models. However, two major methodological differences hinder comparing results across species.

Sinusoidal endotheliitis as a histological parameter for diagnosing acute liver allograft rejection.

Aim: To investigated the feasibility of using sinusoidal endotheliitis (SE) as a histological marker for liver allograft rejection.

Methods: We compared the histological features of 88 liver allograft biopsies with acute cellular rejection (ACR) and 59 cases with no evidence of ACR. SE was scored as: (1) focal linear lifting up of the endothelial cells by lymphocytes with no obvious damage to adjacent hepatocytes; (2) focal disruption of the endothelial lining by a cluster of subendothelial lymphocytes (a group of > 3 lymphocytes); and (3) severe confluent endotheliitis with hemorrhage and adjacent hepatocyte loss.

Resveratrol inhibits enterovirus 71 replication and pro-inflammatory cytokine secretion in rhabdosarcoma cells through blocking IKKs/NF-κB signaling pathway.

Polydatin and resveratrol, as major active components in Polygonum cuspidatum, have anti-inflammatory, antioxidant and antitumor functions. However, the effect and mechanism of polydatin and resveratrol on enterovirus 71 (EV71) have not been reported. In this study, resveratrol revealed strong antiviral activity on EV71, while polydatin had weak effect.

Carbapenem and cefoxitin resistance of Klebsiella pneumoniae strains associated with porin OmpK36 loss and DHA-1 β-lactamase production.

Clinical isolates of carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae) strains are being increased worldwide. Five pan-resistant K.

Chlorogenic acid inhibits the replication and viability of enterovirus 71 in vitro.

Enterovirus 71 (EV71) is an etiology for a number of diseases in humans. Traditional Chinese herbs have been reported to be effective for treating EV71 infection. However, there is no report about the antiviral effects of CHA against EV71.

[Recent advances in gene change of pancreatic cancer].

A large number of data derived from molecular analyses support the hypothesis that human cancer is a genetic disease and a distinct subset of genes have been found to be genetically changed in most tumors. Molecular alterations in pancreatic cancer include: (1) oncogenes such as K-ras, c-myc, c-fos, and c-erbB-2; (2) tumor suppressor genes such as p53, p16, DPC4/SMAD4, and DCC; and (3) growth factors such as EGF, FGF, HGF, PDGF, VEGF, TGF-beta. Genetic alterations of K-ras and p53 are common in human pancreatic cancer, but the occurrence of pancreatic cancer is a multi-step phenomenon in which the accumulation of genetic changes is extremely important.