Cardiac-Specific Gene Editing via an AAV9-Tnnt2-SaCas9-miR122TS Vector.

The adeno-associated virus serotype 9 (AAV9)-delivered gene expression driven by the cardiac troponin T (Tnnt2) promoter is broadly considered to be cardiac-specific. However, in cases where low AAV expression is sufficient to trigger a profound biological effect in CRISPR/Cas9 gene editing, the ectopic AAV9-Tnnt2 expression and gene editing in the liver becomes non-negligible. MicroRNA122 is a microRNA that is specifically expressed in the liver.

Janus micro/nanomotors for enhanced disease treatment through their deep penetration capability.

Nanotherapeutic systems have provided an innovative means for the treatment of a wide range of diseases in modern medicine. However, the limited penetration of nanoparticles into focal tissues still greatly hampered their clinical application. With their unique two-sided structure and superior motility, Janus micro/nanomotors are expected to significantly improve the penetration of nanocarriers into organisms, thereby enhancing the therapeutic effects of diseases.

Targeting membrane contact sites to mediate lipid dynamics: innovative cancer therapies.

Membrane contact sites (MCS) are specialized regions where organelles are closely interconnected through membrane structures, facilitating the transfer and exchange of ions, lipids, and other molecules. This proximity enables a synergistic regulation of cellular homeostasis and functions. The formation and maintenance of these contact sites are governed by specific proteins that bring organelle membranes into close apposition, thereby enabling functional crosstalk between cellular compartments.

One-pot synthesis of surfactant-intercalated tin(IV) disulfide nanosheets heterojuncted with bismuth(III) sulfide needles for efficient conversion carbon dioxide into formate.

Electrocatalytic conversion of carbon dioxide (CO) to formate is an effective strategy for converting CO into valuable chemicals. However, synthesizing active catalysts with well-defined heterojunctions and large exposed surfaces remains challenging. Here, we present a one-pot synthesis method for a hybrid sulfide catalyst featuring surfactant-intercalated tin(IV) disulfide (SnS) nanosheets heterojuncted with bismuth(III) sulfide (BiS) needles.

Multimedia distribution and exchange characteristics of organophosphorus flame retardants in a typical semi-arid city in China.

After the ban on polybrominated diphenyl ethers (PBDEs), the replacement of these compounds with organophosphorus flame retardants (OPFRs) has drawn widespread attention worldwide. In arid/semi-arid regions, characteristics such as low precipitation, frequent strong winds, and low soil organic carbon content potentially influence the environmental fate of OPFRs. In this study, we investigated the concentrations of nine OPFRs in water, sediment, soil, air, and dry deposition in Lanzhou, an arid/semi-arid region in China.

LMNA-related cardiomyopathy: From molecular pathology to cardiac gene therapy.

Background: The genetic variants of LMNA cause an array of diseases that often affect the heart. LMNA-related cardiomyopathy exhibits high-penetrance and early-onset phenotypes that lead to late-stage heart failure or lethal arrhythmia. As a subtype of dilated cardiomyopathy and arrhythmogenic cardiomyopathy, LMNA-related cardiac dysfunction is resistant to existing cardiac therapeutic strategies, leaving a major unmet clinical need in cardiomyopathy management.

Unlocking the Hidden Potential of Cancer Therapy Targeting Lysine Succinylation.

Lysine succinylation is an emerging post-translational modification of proteins. It involves the addition of the succinyl group to lysine residues of target proteins through both enzymatic and non-enzymatic pathways. This modification can alter the structure of the target protein, which, in turn, impacts protein activity and function and is involved in a wide range of diseases.

Amelioration of gap junction dysfunction in a depression model by loganin: Involvement of GSK-3β/β-catenin signaling.

Ethnopharmacological Relevance: Cornus officinalis Sieb. et Zucc has significant neuroprotective activity and has been widely studied for its potential to improve cognitive function. Our team's previous research has found that loganin isolated from Cornus officinalis has an antidepressant effect.

Mechanical Activation of cPLA2 Impedes Fatty Acid β-Oxidation in Vein Grafts.

High-magnitude cyclic stretch from arterial blood pressure significantly contributes to the excessive proliferation and migration of vascular smooth muscle cells (VSMCs), leading to neointima formation in vein grafts. However, the molecular mechanisms remain unclear. This study highlights the critical role of cytosolic Phospholipase A2 (cPLA2)/ Yin Yang 1 (YY1)/ carnitine palmitoyltransferase 1b (CPT1B) signaling in coordinating VSMC mechanical activation by inhibiting fatty acid β-oxidation.

Modified mRNA-based gene editing reveals sarcomere-based regulation of gene expression in human induced-pluripotent stem cell-derived cardiomyocytes.

Mutations in genes coding sarcomere components are the major causes of human inherited cardiomyopathy. Genome editing is widely applied to genetic modification of human pluripotent stem cells (hPSCs) before hPSCs were differentiated into cardiomyocytes to model cardiomyopathy. Whether genetic mutations influence the early hPSC differentiation process or solely the terminally differentiated cardiomyocytes during cardiac pathogenesis remains challenging to distinguish.

Deformable Joule heating electrode based on hybrid layers of silver nanowires and carbon nanotubes and its application in a refreshable multi-cell Braille display.

Stretchable electrodes are an essential component in soft actuator systems. In particular, Joule heating electrodes (JHEs) are required for thermal actuation systems. A highly stretchable, patternable, and low-voltage operating JHE based on hybrid layers of silver nanowires (AgNWs) and carbon nanotubes (CNTs) is reported.

Filamentous Actin in the Nucleus in Triple-Negative Breast Cancer Stem Cells: A Key to Drug-Induced Nucleolar Stress and Stemness Inhibition?

Actin, primarily a cytoplasmic cytoskeleton protein, is transported in and out of the nucleus with the help of actin-binding proteins (ABPs). Actin exists in two forms, i.e.

Detecting and quantifying PM and NO contributions from train and road traffic in the vicinity of a major railway terminal in Dublin, Ireland.

Air pollution from transport hubs is a recognised health concern for local urban inhabitants. Within the domain of transport hubs, significant attention has been given to larger airport and port settings, however concerns have been raised about emissions from urban railway hubs, especially those with diesel trains. This paper presents an approach that adopts low-cost monitoring (LCM) for fixed site monitoring (FSM) to quantify and disaggregate PM and NO contributions from railway station and road traffic on air quality in the vicinity of railway station in Dublin, Ireland.

Annexin A1 binds PDZ and LIM domain 7 to inhibit adipogenesis and prevent obesity.

Obesity is a global issue that warrants the identification of more effective therapeutic targets and a better understanding of the pivotal molecular pathogenesis. Annexin A1 (ANXA1) is known to inhibit phospholipase A2, exhibiting anti-inflammatory activity. However, the specific effects of ANXA1 in obesity and the underlying mechanisms of action remain unclear.

Estimating rainfall intensity based on surveillance audio and deep-learning.

Rainfall data with high spatial and temporal resolutions are essential for urban hydrological modeling. Ubiquitous surveillance cameras can continuously record rainfall events through video and audio, so they have been recognized as potential rain gauges to supplement professional rainfall observation networks. Since video-based rainfall estimation methods can be affected by variable backgrounds and lighting conditions, audio-based approaches could be a supplement without suffering from these conditions.

In vivo proximity proteomics uncovers palmdelphin (PALMD) as a Z-disc-associated mitigator of isoproterenol-induced cardiac injury.

Z-discs are core ultrastructural organizers of cardiomyocytes that modulate many facets of cardiac pathogenesis. Yet a comprehensive proteomic atlas of Z-disc-associated components remain incomplete. Here, we established an adeno-associated virus (AAV)-delivered, cardiomyocyte-specific, proximity-labeling approach to characterize the Z-disc proteome in vivo.

Neutrophil Extracellular Traps in Breast Cancer: Roles in Metastasis and Beyond.

Despite advances in the treatment of breast cancer, the disease continues to exhibit high global morbidity and mortality. The importance of neutrophils in cancer development has been increasingly recognized. Neutrophil extracellular traps (NETs) are web-like structures released into the extracellular space by activated neutrophils, serving as a potential antimicrobial mechanism for capturing and eliminating microorganisms.

Mixed methods examination of risk perception on vaccination intentions: The perspective of doctor-patient communication.

Aim: From the perspective of doctor-patient communication, this research used multiple methods combined natural language processing (NLP), a cross-sectional survey and an online experiment to investigated how risk perception influenced people's vaccination intention.

Methods: In Study 1, we used Python to crawl 335,045 comments about COVID-19 vaccine published in a social media platform Sina Weibo (equivalent of Twitter in China) from 31 December 2020 to 31 December 2021. Text analysis and sentiment analysis was used to examine how vaccination intention, as measured by linguistic features from the LIWC dictionary, changed with individuals' perceptions of pandemic risk.