Analysis of the virulence of a lethal, carbapenem-resistant hypervirulent KPC-33-producing Klebsiella pneumoniae: Emergence of ST11-KL64 hv-CRKP in ICU.

Objective: Hypervirulent and carbapenem-resistant Klebsiella pneumoniae (hv-CRKP) poses a serious threat to public health. Here, we analyse a case of systemic infection caused by a hv-CRKP, which ultimately led to the patient's death from sepsis. And a total of 30 CRKPs were analyzed to elucidate the molecular epidemiological features of CRKPs in the hospital, and to provide a basis for clinical anti-infective therapy.

Study on the inhibition activity and mechanism of Tanreqing against biofilm formation and .

Objective: To evaluate the antibacterial effect of Tanreqing (TRQ) against and its inhibition activity on bacterial biofilm formation and , and to explore the mechanism of the inhibitory effects of TRQ on biofilm formation.

Methods: An biofilm model of was established, and the impact of TRQ on biofilm formation was evaluated using crystal violet staining and scanning electron microscopy (SEM). Furthermore, the clearance effect of TRQ against in the biofilm was assessed using the viable plate counting method; q-RT PCR as used to evaluate the inhibitory effect of different concentrations of TRQ on the expression of biofilm-related genes in ; The activity of quorum sensing signal molecule AI-2 was detected by bioluminescence assay; Meanwhile, a guinea pig lung infection model of was constructed, and after treated with drugs, pathological analysis of lung tissue and determination of bacterial load in lung tissue were performed.