Hybrid paper/PDMS microfluidic device integrated with RNA extraction and recombinase polymerase amplification for detection of norovirus in foods.

Unlabelled: Human norovirus (HuNoV) is recognized as the leading causative agent of foodborne outbreaks of epidemic gastroenteritis. Consequently, there is a high demand for developing point-of-care testing for HuNoV. We developed an origami microfluidic device that facilitates rapid detection of murine norovirus 1 (MNV-1), a surrogate for HuNoV, encompassing the entire process from sample preparation to result visualization.

Determination of Dencichine in in the Forest and Field Using High Performance Liquid Chromatography.

Dencichine is a nonprotein amino acid, an effective ingredient in with hemostatic and anti-inflammatory effects. There are few studies on the effects of regions and cultivation models on the accumulation of dencichine. In the current study, the content of dencichine in collected from its global cultivation and trading center Yunnan, China, (>640 samples) was determined using an optimized high-performance liquid chromatography method coupled with a diode array detector but without derivatization.

A Novel Polysaccharide From Displays Hypoglycemic Activity in a Diabetic Mouse Model.

A novel polysaccharide, Polysaccharide (HRP) was extracted from the edible mushroom . HRP had weight-average molecular weight 1,949 kDa and number-average molecular weight 873 kDa, and its major components were arabinose (0.71%), galactose (12.

Neutral Polysaccharides From With Hypoglycemic Effects in a Type 2 Diabetic Mouse Model.

Neutral polysaccharides (NHSPs) from the mushroom were purified by D301/D152 resin ion-exchange chromatography and DEAE-cellulose anion exchange chromatography. The weight-average molecular weight (M) and number-average molecular weight (Mn) of NHSP were 1,821 and 820.55 kDa, respectively.

A silver@gold nanoparticle tetrahedron biosensor for multiple pesticides detection based on surface-enhanced Raman scattering.

The detection of multiple pesticides in food and environment is of great importance for human health and safety. In this study, the DNA backbone structure and Ag@Au nanoparticles (NPs) to construct a nano-tetrahedron with the help of the surface-enhanced Raman scattering (SERS) effect by controlling the formation of SERS hotspots and subsequently realized the simultaneous detection of multiple pesticides. The DNA aptamers corresponding to the three pesticides of profenofos, acetamiprid and carbendazim were embedded into the three edges of the DNA tetrahedral skeleton, and the tetrahedral corners were connected to modify the Ag@Au NPs with different Raman signaling molecules.

Simultaneous and ultrasensitive detection of three pesticides using a surface-enhanced Raman scattering-based lateral flow assay test strip.

Chlorothalonil (CHL), imidacloprid (IMI) and oxyfluorfen (OXY) are commonly used in combination to increase crop yield. However, these three pesticides are toxic to aquatic organisms and do not easily degrade. In this study, a surface-enhanced Raman scattering-based lateral flow assay (SERS-LFA) test strip was prepared by combining antibodies with SERS nanotags, and then competitive immune binding was used to detect the three pesticides simultaneously.

Oxidase-mimicking activity of ultrathin MnO nanosheets in a colorimetric assay of chlorothalonil in food samples.

Chlorothalonil is a class of 2B carcinogen which is widely used in the prevention and treatment of fungal diseases in food samples. Its residual problem has been increasingly concerned by society. In this paper, a fast and simple colorimetric assay based on Manganese dioxide nanosheets (MnO NSs)-oxidize 3,3',5,5'-tetramethylbenzidine (TMB) platform was used to detect residual pesticide chlorothalonil in food samples.

Ratiometric Fluorescent Quantum Dot-Based Biosensor for Chlorothalonil Detection via an Inner-Filter Effect.

A new sensitive sensor for detecting chlorothalonil (CHL) based on the inner-filter effect (IFE) between gold nanoparticles (AuNPs) and ratiometric fluorescent quantum dots (RF-QDs) was developed. Here, RF-QDs were designed by two different color CdTe QDs. Based on the IFE, the AuNPs can quench the fluorescence of the RF-QDs.

Solving NP-Hard Problems with Physarum-Based Ant Colony System.

NP-hard problems exist in many real world applications. Ant colony optimization (ACO) algorithms can provide approximate solutions for those NP-hard problems, but the performance of ACO algorithms is significantly reduced due to premature convergence and weak robustness, etc. With these observations in mind, this paper proposes a Physarum-based pheromone matrix optimization strategy in ant colony system (ACS) for solving NP-hard problems such as traveling salesman problem (TSP) and 0/1 knapsack problem (0/1 KP).

Multi-Objective Ant Colony Optimization Based on the Physarum-Inspired Mathematical Model for Bi-Objective Traveling Salesman Problems.

Bi-objective Traveling Salesman Problem (bTSP) is an important field in the operations research, its solutions can be widely applied in the real world. Many researches of Multi-objective Ant Colony Optimization (MOACOs) have been proposed to solve bTSPs. However, most of MOACOs suffer premature convergence.

Characterization of a novel laccase purified from the fungus Hohenbuehelia serotina and its decolourisation of dyes.

A novel laccase was purified from the white rot fungus, Hohenbuehelia serotina, to investigate the applications of this laccase in the decoloration of various dyes. SDS-PAGE revealed a single band of this laccase corresponding to a molecular weight of approximately 57.8 kDa.