Machine learning based on multiplatform tests assists in subtype classification of mature B-cell neoplasms.

Mature B-cell neoplasms (MBNs) are clonal proliferative diseases encompassing over 40 subtypes. The WHO classification (morphology, immunology, cytogenetics and molecular biology) provides comprehensive diagnostic understandings. However, MBN subtyping relies heavily on the expertise of clinicians and pathologists, and differences in clinical experience can lead to variations in subtyping efficiency and consistency.

Effects of additive dosage and coagulation bath pH on amphoteric fluorocarbon special surfactant (FS-50) blend PVDF membranes.

Amphiphilic copolymers containing hydrophilic and hydrophobic blocks represented by surfactants have proven to be more effective for modifying membranes than hydrophilic copolymers. However, studies on the effects of additive and coagulation bath pH on the morphology and properties of surfactant-modified membranes have rarely been reported. Hence, this study aims to investigate the effects of the additive dosage and the coagulation bath pH on the mechanisms of phase inversion and performance improvement of amphoteric fluorocarbon special surfactant (FS-50) blended PVDF membranes.

Extracellular Production, Characterization, and Engineering of a Polyextremotolerant Subtilisin-Like Protease From Feather-Degrading Strain CDF.

Here, the gene encoding a subtilisin-like protease (protease Als) was cloned from strain CDF and expressed in . The recombinant enzyme was released into the culture medium of as a mature form (mAls). Purified mAls displayed optimal activity at 60-70°C and pH 10.

Parallel Analyses of Somatic Mutations in Plasma Circulating Tumor DNA (ctDNA) and Matched Tumor Tissues in Early-Stage Breast Cancer.

Purpose: Early detection and intervention can decrease the mortality of breast cancer significantly. Assessments of genetic/genomic variants in circulating tumor DNA (ctDNA) have generated great enthusiasm for their potential application as clinically actionable biomarkers in the management of early-stage breast cancer. In this study, 861 serial plasma and matched tissue specimens from 102 patients with early-stage breast cancer who need chemotherapy and 50 individuals with benign breast tumors were deeply sequenced via next-generation sequencing (NGS) techniques using large gene panels.

Complete Genome Sequence of Thermoactinomyces vulgaris Strain CDF, a Thermophilic Bacterium Capable of Degrading Chicken Feathers.

strain CDF was isolated from soil and shown to have the ability to degrade chicken feathers at high temperatures. Here, we report the complete genome sequence of this bacterium, which is 2,595,509 bp long with 2,642 predicted genes and an average G+C content of 48.14%.

[Enhanced Coagulation as a Pretreatment for Low Temperature Wastewater].

The effects of low temperature on enhanced coagulation were studied. A new composite coagulant called SynthA was synthesized. The effects of enhanced coagulation on the removals of dissolved organic matter, dissolved organic nitrogen, and so on under room temperature or low temperature (2-5℃) were determined, and their influences on biological treatments were investigated by using membrane fractionation distribution, three-dimensional fluorescence spectrum (3DEEM), and differential ultraviolent absorbance.

Autocatalytic activation of a thermostable glutamyl endopeptidase capable of hydrolyzing proteins at high temperatures.

Glutamyl endopeptidases (GSEs) specifically hydrolyze peptide bonds formed by α-carboxyl groups of Glu and Asp residues. We cloned the gene for a thermophilic GSE (designated TS-GSE) from Thermoactinomyces sp. CDF.

Degradation of intact chicken feathers by Thermoactinomyces sp. CDF and characterization of its keratinolytic protease.

Thermoactinomyces is known for its resistance to extreme environmental conditions and its ability to digest a wide range of hard-to-degrade compounds. Here, Thermoactinomyces sp. strain CDF isolated from soil was found to completely degrade intact chicken feathers at 55 °C, with the resulting degradation products sufficient to support growth as the primary source of both carbon and nitrogen.

Expression plasmids for use in Candida glabrata.

We describe a series of CEN/ARS episomal plasmids containing different Candida glabrata promoters, allowing for a range of constitutive or regulated expression of proteins in C. glabrata. The set of promoters includes three constitutive promoters (EGD2pr, HHT2pr, PDC1pr), two macrophage/phagocytosis-induced promoters (ACO2pr, LYS21pr), and one nutritionally regulated promoter (MET3pr).